The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity
Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecul...
| Autor principal: | Ferreira, Milena |
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| Outros Autores: | Garcia, Caroline, Matoso, D. A., Jesus, Isac Silva de, Cioffi, Marcelo de Bello, Bertollo, Luiz Antônio Carlos, Zuanon, Jansen, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Frontiers in Genetics
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15711 |
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oai:repositorio:1-15711 |
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oai:repositorio:1-15711 The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity Ferreira, Milena Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg. 2020-05-18T15:08:10Z 2020-05-18T15:08:10Z 2017 Artigo https://repositorio.inpa.gov.br/handle/1/15711 10.3389/fgene.2017.00120 en Volume 8, Número SEP Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Frontiers in Genetics |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis |
| spellingShingle |
Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis Ferreira, Milena The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| topic_facet |
Dna 18s Ribosome Dna Rna 5s Biodiversity Bunocephalus Coracoideus Catfish Chromosome Analysis Cytogenetics Dna Barcoding Dna Denaturation Dna Sequence Evolution In Situ Hybridization, Fluorescence Gene Amplification Genetic Variability Image Processing Kidney Cell Nonhuman Phylogeny Polymerase Chain Reaction Testis |
| description |
Bunocephalus is the most species-rich Aspredinidae genus, corresponding to a monophyletic clade with 13 valid species. However, many species have their classification put in question. Here, we analyzed individuals from four Amazonian populations of Bunocephalus coracoideus by cytogenetic and molecular procedures. The geographic distribution, genetic distances and karyotype data indicate that each population represents an Evolutionary Significant Unit (ESU). Cytogenetic markers showed distinct 2n and karyotype formulas, as well as different numbers and locations of the rDNA sites among ESUs. One of such populations (ESU-D) highlighted an extensive polymorphic condition, with several cytotypes probably due to chromosomal rearrangements and meiotic non-disjunctions. This resulted in several aneuploid karyotypes, which was also supported by the mapping of telomeric sequences. Phylograms based on Maximum Likelihood (ML) and Neighbor Joining (NJ) analyses grouped each ESU on particular highly supported clades, with the estimation of evolutionary divergence indicating values being higher than 3.8-12.3% among them. Our study reveals a huge degree of chromosomal and genetic diversity in B. coracoideus and highly points to the existence of four ESUs in allopatric and sympatric speciation processes. In fact, the high divergences found among the ESUs allowed us to delimitate lineages with taxonomic uncertainties in this nominal species. © 2017 Ferreira, Garcia, Matoso, de Jesus, Cioffi, Bertollo, Zuanon and Feldberg. |
| format |
Artigo |
| author |
Ferreira, Milena |
| author2 |
Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana |
| author2Str |
Garcia, Caroline Matoso, D. A. Jesus, Isac Silva de Cioffi, Marcelo de Bello Bertollo, Luiz Antônio Carlos Zuanon, Jansen Feldberg, Eliana |
| title |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| title_short |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| title_full |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| title_fullStr |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| title_full_unstemmed |
The Bunocephalus coracoideus species complex (Siluriformes, Aspredinidae). Signs of a speciation process through chromosomal, genetic and ecological diversity |
| title_sort |
bunocephalus coracoideus species complex (siluriformes, aspredinidae). signs of a speciation process through chromosomal, genetic and ecological diversity |
| publisher |
Frontiers in Genetics |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15711 |
| _version_ |
1787141475290578944 |
| score |
11.675088 |