Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats
Sertoli cells (SCs) play a crucial role in testis differentiation, development and function, determining the magnitude of sperm production in sexually mature animals. For over 40 years, it has been considered that these key testis somatic cells stop dividing during early pre-pubertal phase, between...
| Autor principal: | Figueiredo, Andre´ Felipe Almeida |
|---|---|
| Outros Autores: | França, Luiz Renato de, Hess, Rex Allen, Costa, Guilherme M.J. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Cell Cycle
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15800 |
| id |
oai:repositorio:1-15800 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-15800 Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats Figueiredo, Andre´ Felipe Almeida França, Luiz Renato de Hess, Rex Allen Costa, Guilherme M.J. Androgen Receptor Broxuridine Ki 67 Antigen Protein P27 Transcription Factor Gata 4 Androgen Receptor Biological Marker Broxuridine Ki 67 Antigen Transcription Factor Gata 4 Adult Adulthood Animals Cell Animals Experiment Cell Differentiation Cell Proliferation Male Nonhuman Phenotype Prepuberty Protein Expression Rat Rete Testis Seminiferous Tubule Sertoli Cell Testis Function Aging Animals Cell Proliferation Cytology Fluorescent Antibody Technique Metabolism Physiology Rete Testis Seminiferous Tubule Sertoli Cell Sexual Maturation Wistar Rat Aging Animal Biomarkers Bromodeoxyuridine Cell Differentiation Cell Proliferation Fluorescent Antibody Technique Gata4 Transcription Factor Ki 67 Antigen Male Rats, Wistar Receptors, Androgen Rete Testis Seminiferous Tubules Sertoli Cells Sexual Maturation Sertoli cells (SCs) play a crucial role in testis differentiation, development and function, determining the magnitude of sperm production in sexually mature animals. For over 40 years, it has been considered that these key testis somatic cells stop dividing during early pre-pubertal phase, between around 10 to 20 days after birth respectively in mice and rats, being after that under physiological conditions a stable and terminally differentiated population. However, evidences from the literature are challenging this dogma. In the present study, using several important functional markers (Ki-67, BrdU, p27, GATA-4, Androgen Receptor), we investigated the SC differentiation status in 36 days old and adult Wistar rats, focusing mainly in the transition region (TR) between the seminiferous tubules (ST) and the rete testis. Our results showed that SCs in TR remain undifferentiated for a longer period and, although at a lesser degree, even in adult rats proliferating SCs were observed in this region. Therefore, these findings suggest that, different from the other ST regions investigated, SCs residing in the TR exhibit a distinct functional phenotype. These undifferentiated SCs may compose a subpopulation of SC progenitors that reside in a specific microenvironment capable of growing the ST length if needed from this particular testis region. Moreover, our findings demonstrate an important aspect of testis function in mammals and opens new venues for other experimental approaches to the investigation of SC physiology, spermatogenesis progression and testis growth. Besides that, the TR may represent an important site for pathophysiological investigations and cellular interactions in the testis. © 2016 Taylor & Francis. 2020-05-18T21:21:06Z 2020-05-18T21:21:06Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/15800 10.1080/15384101.2016.1207835 en Volume 15, Número 18, Pags. 2486-2496 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Cell Cycle |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Androgen Receptor Broxuridine Ki 67 Antigen Protein P27 Transcription Factor Gata 4 Androgen Receptor Biological Marker Broxuridine Ki 67 Antigen Transcription Factor Gata 4 Adult Adulthood Animals Cell Animals Experiment Cell Differentiation Cell Proliferation Male Nonhuman Phenotype Prepuberty Protein Expression Rat Rete Testis Seminiferous Tubule Sertoli Cell Testis Function Aging Animals Cell Proliferation Cytology Fluorescent Antibody Technique Metabolism Physiology Rete Testis Seminiferous Tubule Sertoli Cell Sexual Maturation Wistar Rat Aging Animal Biomarkers Bromodeoxyuridine Cell Differentiation Cell Proliferation Fluorescent Antibody Technique Gata4 Transcription Factor Ki 67 Antigen Male Rats, Wistar Receptors, Androgen Rete Testis Seminiferous Tubules Sertoli Cells Sexual Maturation |
| spellingShingle |
Androgen Receptor Broxuridine Ki 67 Antigen Protein P27 Transcription Factor Gata 4 Androgen Receptor Biological Marker Broxuridine Ki 67 Antigen Transcription Factor Gata 4 Adult Adulthood Animals Cell Animals Experiment Cell Differentiation Cell Proliferation Male Nonhuman Phenotype Prepuberty Protein Expression Rat Rete Testis Seminiferous Tubule Sertoli Cell Testis Function Aging Animals Cell Proliferation Cytology Fluorescent Antibody Technique Metabolism Physiology Rete Testis Seminiferous Tubule Sertoli Cell Sexual Maturation Wistar Rat Aging Animal Biomarkers Bromodeoxyuridine Cell Differentiation Cell Proliferation Fluorescent Antibody Technique Gata4 Transcription Factor Ki 67 Antigen Male Rats, Wistar Receptors, Androgen Rete Testis Seminiferous Tubules Sertoli Cells Sexual Maturation Figueiredo, Andre´ Felipe Almeida Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| topic_facet |
Androgen Receptor Broxuridine Ki 67 Antigen Protein P27 Transcription Factor Gata 4 Androgen Receptor Biological Marker Broxuridine Ki 67 Antigen Transcription Factor Gata 4 Adult Adulthood Animals Cell Animals Experiment Cell Differentiation Cell Proliferation Male Nonhuman Phenotype Prepuberty Protein Expression Rat Rete Testis Seminiferous Tubule Sertoli Cell Testis Function Aging Animals Cell Proliferation Cytology Fluorescent Antibody Technique Metabolism Physiology Rete Testis Seminiferous Tubule Sertoli Cell Sexual Maturation Wistar Rat Aging Animal Biomarkers Bromodeoxyuridine Cell Differentiation Cell Proliferation Fluorescent Antibody Technique Gata4 Transcription Factor Ki 67 Antigen Male Rats, Wistar Receptors, Androgen Rete Testis Seminiferous Tubules Sertoli Cells Sexual Maturation |
| description |
Sertoli cells (SCs) play a crucial role in testis differentiation, development and function, determining the magnitude of sperm production in sexually mature animals. For over 40 years, it has been considered that these key testis somatic cells stop dividing during early pre-pubertal phase, between around 10 to 20 days after birth respectively in mice and rats, being after that under physiological conditions a stable and terminally differentiated population. However, evidences from the literature are challenging this dogma. In the present study, using several important functional markers (Ki-67, BrdU, p27, GATA-4, Androgen Receptor), we investigated the SC differentiation status in 36 days old and adult Wistar rats, focusing mainly in the transition region (TR) between the seminiferous tubules (ST) and the rete testis. Our results showed that SCs in TR remain undifferentiated for a longer period and, although at a lesser degree, even in adult rats proliferating SCs were observed in this region. Therefore, these findings suggest that, different from the other ST regions investigated, SCs residing in the TR exhibit a distinct functional phenotype. These undifferentiated SCs may compose a subpopulation of SC progenitors that reside in a specific microenvironment capable of growing the ST length if needed from this particular testis region. Moreover, our findings demonstrate an important aspect of testis function in mammals and opens new venues for other experimental approaches to the investigation of SC physiology, spermatogenesis progression and testis growth. Besides that, the TR may represent an important site for pathophysiological investigations and cellular interactions in the testis. © 2016 Taylor & Francis. |
| format |
Artigo |
| author |
Figueiredo, Andre´ Felipe Almeida |
| author2 |
França, Luiz Renato de Hess, Rex Allen Costa, Guilherme M.J. |
| author2Str |
França, Luiz Renato de Hess, Rex Allen Costa, Guilherme M.J. |
| title |
Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| title_short |
Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| title_full |
Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| title_fullStr |
Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| title_full_unstemmed |
Sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in Wistar rats |
| title_sort |
sertoli cells are capable of proliferation into adulthood in the transition region between the seminiferous tubules and the rete testis in wistar rats |
| publisher |
Cell Cycle |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15800 |
| _version_ |
1787145418120888320 |
| score |
11.653393 |