Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory
Background: The Amazon is the largest rainforest in the world and is home to a rich biodiversity of medicinal plants. Several of these plants are used by the local population for the treatment of diseases, many of those with probable anti-inflammatory effect. The aim of the present investigation was...
| Autor principal: | Vargas, Fabiano S. de |
|---|---|
| Outros Autores: | Almeida, Patrícia Danielle Oliveira de, Boleti, Ana Paula A. de, Pereira, Maria de Meneses, Souza, Tatiane Pereira de, Vasconcellos, Marne Carvalho de, Nunez, C. V., Pohlit, Adrian Martin, Lima, Emerson Silva |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
BMC Complementary and Alternative Medicine
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15834 |
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oai:repositorio:1-15834 |
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oai:repositorio:1-15834 Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory Vargas, Fabiano S. de Almeida, Patrícia Danielle Oliveira de Boleti, Ana Paula A. de Pereira, Maria de Meneses Souza, Tatiane Pereira de Vasconcellos, Marne Carvalho de Nunez, C. V. Pohlit, Adrian Martin Lima, Emerson Silva Antioxidant Byrsonima Japurensis Plant Extract Calycophyllum Spruceanum Plant Extract Enzyme Inhibitor Horseradish Peroxidase Maytenus Guyanensis Plant Extract Myeloperoxidase Passiflora Nitida Plant Extract Ptychopetalum Olacoides Plant Extract Unclassified Drug Antiinflammatory Agent Antioxidant Peroxidase Plant Extract Polyphenol Animals Cell Antiinflammatory Activity Antioxidant Activity Bleaching Byrsonima Japurensis Calycophyllum Spruceanum Concentration Response Controlled Study Drug Screening Enzyme Inhibition Fibroblast Maytenus Guyanensis Medicinal Plant Mouse Nonhuman Passiflora Nitida Plant Stem Ptychopetalum Olacoides Animals Antagonists And Inhibitors Brasil Chemistry Human Malpighiaceae Maytenus Nih 3t3 Cell Line Olacaceae Passiflora Phytotherapy Medicine, Traditional Animal Anti-inflammatory Agents Antioxidants Brasil Humans Malpighiaceae Maytenus Medicine, Traditional Mice Nih 3t3 Cells Olacaceae Passiflora Peroxidase Peroxidases Phytotherapy Plant Extracts Plants, Medicinal Polyphenols Background: The Amazon is the largest rainforest in the world and is home to a rich biodiversity of medicinal plants. Several of these plants are used by the local population for the treatment of diseases, many of those with probable anti-inflammatory effect. The aim of the present investigation was to evaluate the in vitro antioxidant and anti-peroxidases potential of the ethanol extracts of five plants from the Brazilian Amazon (Byrsonima japurensis, Calycophyllum spruceanum, Maytenus guyanensis, Passiflora nitida and Ptychopetalum olacoides). Methods: DPPH, ABTS, superoxide anion radical, singlet oxygen and the β-carotene bleaching methods were employed for characterization of free radical scavenging activity. Also, total polyphenols were determined. Antioxidant activities were evaluated using murine fibroblast NIH3T3 cell. Inhibition of HRP and MPO were evaluated using amplex red® as susbtract. Results: The stem bark extracts of C. spruceanum and M. guyanensis provided the highest free radical scavenging activities. C. spruceanum exhibited IC50 = 7.5 ± 0.9, 5.0 ± 0.1, 18.2 ± 3.0 and 92.4 ± 24.8 μg/mL for DPPH·, ABTS+·, O2 -· and 1O2 assays, respectively. P. olacoides and C. spruceanum extracts also inhibited free radicals formation in the cell-based assay. At a concentration of 100 μg/mL, the extracts of C. spruceanum, B. japurensis inhibited horseradish peroxidase by 62 and 50 %, respectively. C. spruceanum, M. guyanensis, B. japurensis also inhibited myeloperoxidase in 72, 67 and 56 %, respectively. Conclusions: This work supports the folk use these species that inhibited peroxidases and exhibited significant free radical scavenging and antioxidant activities what can be related to treatment of inflammation. © 2016 de Vargas et al. 2020-05-19T14:25:47Z 2020-05-19T14:25:47Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/15834 10.1186/s12906-016-1061-9 en Volume 16, Número 1 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf BMC Complementary and Alternative Medicine |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Antioxidant Byrsonima Japurensis Plant Extract Calycophyllum Spruceanum Plant Extract Enzyme Inhibitor Horseradish Peroxidase Maytenus Guyanensis Plant Extract Myeloperoxidase Passiflora Nitida Plant Extract Ptychopetalum Olacoides Plant Extract Unclassified Drug Antiinflammatory Agent Antioxidant Peroxidase Plant Extract Polyphenol Animals Cell Antiinflammatory Activity Antioxidant Activity Bleaching Byrsonima Japurensis Calycophyllum Spruceanum Concentration Response Controlled Study Drug Screening Enzyme Inhibition Fibroblast Maytenus Guyanensis Medicinal Plant Mouse Nonhuman Passiflora Nitida Plant Stem Ptychopetalum Olacoides Animals Antagonists And Inhibitors Brasil Chemistry Human Malpighiaceae Maytenus Nih 3t3 Cell Line Olacaceae Passiflora Phytotherapy Medicine, Traditional Animal Anti-inflammatory Agents Antioxidants Brasil Humans Malpighiaceae Maytenus Medicine, Traditional Mice Nih 3t3 Cells Olacaceae Passiflora Peroxidase Peroxidases Phytotherapy Plant Extracts Plants, Medicinal Polyphenols |
| spellingShingle |
Antioxidant Byrsonima Japurensis Plant Extract Calycophyllum Spruceanum Plant Extract Enzyme Inhibitor Horseradish Peroxidase Maytenus Guyanensis Plant Extract Myeloperoxidase Passiflora Nitida Plant Extract Ptychopetalum Olacoides Plant Extract Unclassified Drug Antiinflammatory Agent Antioxidant Peroxidase Plant Extract Polyphenol Animals Cell Antiinflammatory Activity Antioxidant Activity Bleaching Byrsonima Japurensis Calycophyllum Spruceanum Concentration Response Controlled Study Drug Screening Enzyme Inhibition Fibroblast Maytenus Guyanensis Medicinal Plant Mouse Nonhuman Passiflora Nitida Plant Stem Ptychopetalum Olacoides Animals Antagonists And Inhibitors Brasil Chemistry Human Malpighiaceae Maytenus Nih 3t3 Cell Line Olacaceae Passiflora Phytotherapy Medicine, Traditional Animal Anti-inflammatory Agents Antioxidants Brasil Humans Malpighiaceae Maytenus Medicine, Traditional Mice Nih 3t3 Cells Olacaceae Passiflora Peroxidase Peroxidases Phytotherapy Plant Extracts Plants, Medicinal Polyphenols Vargas, Fabiano S. de Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| topic_facet |
Antioxidant Byrsonima Japurensis Plant Extract Calycophyllum Spruceanum Plant Extract Enzyme Inhibitor Horseradish Peroxidase Maytenus Guyanensis Plant Extract Myeloperoxidase Passiflora Nitida Plant Extract Ptychopetalum Olacoides Plant Extract Unclassified Drug Antiinflammatory Agent Antioxidant Peroxidase Plant Extract Polyphenol Animals Cell Antiinflammatory Activity Antioxidant Activity Bleaching Byrsonima Japurensis Calycophyllum Spruceanum Concentration Response Controlled Study Drug Screening Enzyme Inhibition Fibroblast Maytenus Guyanensis Medicinal Plant Mouse Nonhuman Passiflora Nitida Plant Stem Ptychopetalum Olacoides Animals Antagonists And Inhibitors Brasil Chemistry Human Malpighiaceae Maytenus Nih 3t3 Cell Line Olacaceae Passiflora Phytotherapy Medicine, Traditional Animal Anti-inflammatory Agents Antioxidants Brasil Humans Malpighiaceae Maytenus Medicine, Traditional Mice Nih 3t3 Cells Olacaceae Passiflora Peroxidase Peroxidases Phytotherapy Plant Extracts Plants, Medicinal Polyphenols |
| description |
Background: The Amazon is the largest rainforest in the world and is home to a rich biodiversity of medicinal plants. Several of these plants are used by the local population for the treatment of diseases, many of those with probable anti-inflammatory effect. The aim of the present investigation was to evaluate the in vitro antioxidant and anti-peroxidases potential of the ethanol extracts of five plants from the Brazilian Amazon (Byrsonima japurensis, Calycophyllum spruceanum, Maytenus guyanensis, Passiflora nitida and Ptychopetalum olacoides). Methods: DPPH, ABTS, superoxide anion radical, singlet oxygen and the β-carotene bleaching methods were employed for characterization of free radical scavenging activity. Also, total polyphenols were determined. Antioxidant activities were evaluated using murine fibroblast NIH3T3 cell. Inhibition of HRP and MPO were evaluated using amplex red® as susbtract. Results: The stem bark extracts of C. spruceanum and M. guyanensis provided the highest free radical scavenging activities. C. spruceanum exhibited IC50 = 7.5 ± 0.9, 5.0 ± 0.1, 18.2 ± 3.0 and 92.4 ± 24.8 μg/mL for DPPH·, ABTS+·, O2 -· and 1O2 assays, respectively. P. olacoides and C. spruceanum extracts also inhibited free radicals formation in the cell-based assay. At a concentration of 100 μg/mL, the extracts of C. spruceanum, B. japurensis inhibited horseradish peroxidase by 62 and 50 %, respectively. C. spruceanum, M. guyanensis, B. japurensis also inhibited myeloperoxidase in 72, 67 and 56 %, respectively. Conclusions: This work supports the folk use these species that inhibited peroxidases and exhibited significant free radical scavenging and antioxidant activities what can be related to treatment of inflammation. © 2016 de Vargas et al. |
| format |
Artigo |
| author |
Vargas, Fabiano S. de |
| author2 |
Almeida, Patrícia Danielle Oliveira de Boleti, Ana Paula A. de Pereira, Maria de Meneses Souza, Tatiane Pereira de Vasconcellos, Marne Carvalho de Nunez, C. V. Pohlit, Adrian Martin Lima, Emerson Silva |
| author2Str |
Almeida, Patrícia Danielle Oliveira de Boleti, Ana Paula A. de Pereira, Maria de Meneses Souza, Tatiane Pereira de Vasconcellos, Marne Carvalho de Nunez, C. V. Pohlit, Adrian Martin Lima, Emerson Silva |
| title |
Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| title_short |
Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| title_full |
Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| title_fullStr |
Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| title_full_unstemmed |
Antioxidant activity and peroxidase inhibition of Amazonian plants extracts traditionally used as anti-inflammatory |
| title_sort |
antioxidant activity and peroxidase inhibition of amazonian plants extracts traditionally used as anti-inflammatory |
| publisher |
BMC Complementary and Alternative Medicine |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15834 |
| _version_ |
1787144112631185408 |
| score |
11.653393 |