Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis
Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and...
| Autor principal: | Silva, Roberto Moreira da |
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| Outros Autores: | Silva Neto, João Ricardo da, Santos, Carla Silvana, Cruz, Kátia Santana, Frickmann, Hagen, Poppert, Sven, Koshikene, Daniela, Souza, João Vicente Braga de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Medical Mycology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/15881 |
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oai:repositorio:1-15881 |
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oai:repositorio:1-15881 Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis Silva, Roberto Moreira da Silva Neto, João Ricardo da Santos, Carla Silvana Cruz, Kátia Santana Frickmann, Hagen Poppert, Sven Koshikene, Daniela Souza, João Vicente Braga de Fluorescent Dyes Ribosome Rna Fungal Rna Oligonucleotide Probe Ribosome Rna Ajellomyces Capsulatus Bacteria Blood Culture Clinical In Situ Hybridization, Fluorescence Fungal Detection Fungal Strain Fungus Identification Histoplasmosis Human Oligonucleotide Probe Polymerase Chain Reaction Yeast Blood In Situ Hybridization, Fluorescence Genetics Histoplasma Histoplasmosis Isolation And Purification Microbiology Molecular Diagnosis Procedures Sensitivity And Specificity Time Candida Histoplasma Capsulatum Var. Capsulatum Blood Histoplasma Histoplasmosis Humans In Situ Hybridization, Fluorescence Molecular Diagnostic Techniques Oligonucleotide Probes Fungal Rna Rna, Ribosomal Sensitivity And Specificity Time Factors Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultures. All three analyzed H. capsulatum reference strains and clinical isolates showed positive signals with the newly designed specific oligonucleotide probes for H. capsulatum, whereas negative reactions were observed for all three nontarget yeast species and the two nontarget bacteria. The assay was also successfully applied for detections of H. capsulatum cells in pre-incubated blood culture samples of patients with clinical suspicion of histoplasmosis (n = 33). The described FISH-based assay was shown to be easy to apply, sensitive, and specific (compared to polymerase chain reaction) for the detection and identification of H. capsulatum in this proof-of-principle analysis. Larger multicentric assessments are recommended for a thorough diagnostic evaluation of the procedure. © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. 2020-05-19T20:34:05Z 2020-05-19T20:34:05Z 2015 Artigo https://repositorio.inpa.gov.br/handle/1/15881 10.1093/mmy/myu080 en Volume 53, Número 2, Pags. 160-164 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Medical Mycology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Fluorescent Dyes Ribosome Rna Fungal Rna Oligonucleotide Probe Ribosome Rna Ajellomyces Capsulatus Bacteria Blood Culture Clinical In Situ Hybridization, Fluorescence Fungal Detection Fungal Strain Fungus Identification Histoplasmosis Human Oligonucleotide Probe Polymerase Chain Reaction Yeast Blood In Situ Hybridization, Fluorescence Genetics Histoplasma Histoplasmosis Isolation And Purification Microbiology Molecular Diagnosis Procedures Sensitivity And Specificity Time Candida Histoplasma Capsulatum Var. Capsulatum Blood Histoplasma Histoplasmosis Humans In Situ Hybridization, Fluorescence Molecular Diagnostic Techniques Oligonucleotide Probes Fungal Rna Rna, Ribosomal Sensitivity And Specificity Time Factors |
| spellingShingle |
Fluorescent Dyes Ribosome Rna Fungal Rna Oligonucleotide Probe Ribosome Rna Ajellomyces Capsulatus Bacteria Blood Culture Clinical In Situ Hybridization, Fluorescence Fungal Detection Fungal Strain Fungus Identification Histoplasmosis Human Oligonucleotide Probe Polymerase Chain Reaction Yeast Blood In Situ Hybridization, Fluorescence Genetics Histoplasma Histoplasmosis Isolation And Purification Microbiology Molecular Diagnosis Procedures Sensitivity And Specificity Time Candida Histoplasma Capsulatum Var. Capsulatum Blood Histoplasma Histoplasmosis Humans In Situ Hybridization, Fluorescence Molecular Diagnostic Techniques Oligonucleotide Probes Fungal Rna Rna, Ribosomal Sensitivity And Specificity Time Factors Silva, Roberto Moreira da Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| topic_facet |
Fluorescent Dyes Ribosome Rna Fungal Rna Oligonucleotide Probe Ribosome Rna Ajellomyces Capsulatus Bacteria Blood Culture Clinical In Situ Hybridization, Fluorescence Fungal Detection Fungal Strain Fungus Identification Histoplasmosis Human Oligonucleotide Probe Polymerase Chain Reaction Yeast Blood In Situ Hybridization, Fluorescence Genetics Histoplasma Histoplasmosis Isolation And Purification Microbiology Molecular Diagnosis Procedures Sensitivity And Specificity Time Candida Histoplasma Capsulatum Var. Capsulatum Blood Histoplasma Histoplasmosis Humans In Situ Hybridization, Fluorescence Molecular Diagnostic Techniques Oligonucleotide Probes Fungal Rna Rna, Ribosomal Sensitivity And Specificity Time Factors |
| description |
Fluorescence in situ hybridization (FISH) has been shown to be useful for the detection of Candida and Cryptococcus species in blood culture materials. FISH procedures for the detection of Histoplasma capsulatum var. capsulatum have not been reported so far. This study describes the development and evaluation of fluorescently labeled rRNA-targeting FISH probes to detect and identify H. capsulatum in blood cultures. All three analyzed H. capsulatum reference strains and clinical isolates showed positive signals with the newly designed specific oligonucleotide probes for H. capsulatum, whereas negative reactions were observed for all three nontarget yeast species and the two nontarget bacteria. The assay was also successfully applied for detections of H. capsulatum cells in pre-incubated blood culture samples of patients with clinical suspicion of histoplasmosis (n = 33). The described FISH-based assay was shown to be easy to apply, sensitive, and specific (compared to polymerase chain reaction) for the detection and identification of H. capsulatum in this proof-of-principle analysis. Larger multicentric assessments are recommended for a thorough diagnostic evaluation of the procedure. © The Author 2014. Published by Oxford University Press on behalf of The International Society for Human and Animal Mycology. |
| format |
Artigo |
| author |
Silva, Roberto Moreira da |
| author2 |
Silva Neto, João Ricardo da Santos, Carla Silvana Cruz, Kátia Santana Frickmann, Hagen Poppert, Sven Koshikene, Daniela Souza, João Vicente Braga de |
| author2Str |
Silva Neto, João Ricardo da Santos, Carla Silvana Cruz, Kátia Santana Frickmann, Hagen Poppert, Sven Koshikene, Daniela Souza, João Vicente Braga de |
| title |
Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| title_short |
Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| title_full |
Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| title_fullStr |
Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| title_full_unstemmed |
Fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| title_sort |
fluorescent in situ hybridization of pre-incubated blood culture material for the rapid diagnosis of histoplasmosis |
| publisher |
Medical Mycology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/15881 |
| _version_ |
1787144888623562752 |
| score |
11.675088 |