Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents
The Scutellaria agrestis is used by Amazonas riverine communities, especially for otitis externa topical treatment, by using the crude extract obtained by maceration. This study aimed to investigate the preliminary phytochemical profile, the safety/toxicity and the analgesic, anti-inflammatory and a...
| Autor principal: | Oliveira, A. B. |
|---|---|
| Outros Autores: | Barbosa, Gleyce dos Santos, Verdam, Maria Christina dos Santos, Ohana, Débora Teixeira, Mendonça, Maria Silvia de, Alves Meira, Renata Maria Strozi |
| Grau: | Artigo |
| Idioma: | pt_BR |
| Publicado em: |
Revista Brasileira de Plantas Medicinais
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16000 |
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oai:repositorio:1-16000 |
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oai:repositorio:1-16000 Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents Efeito analgésico e anti-inflamatório do extrato aquoso das folhas de trevo-roxo (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) em roedores Oliveira, A. B. Barbosa, Gleyce dos Santos Verdam, Maria Christina dos Santos Ohana, Débora Teixeira Mendonça, Maria Silvia de Alves Meira, Renata Maria Strozi Alkaloid Cyanogenic Heteroside Phenol Derivative Plant Extract Plant Medicinal Product Scutellaria Agrestis A Extarct Terpene Unclassified Drug Analgesic Activity Animals Experiment Animals Model Antiedematogenic Activity Antiinflammatory Activity Biological Activity Controlled Study External Otitis Mouse Nonhuman Paw Edema Phytochemistry Scutellaria Toxicity Testing The Scutellaria agrestis is used by Amazonas riverine communities, especially for otitis externa topical treatment, by using the crude extract obtained by maceration. This study aimed to investigate the preliminary phytochemical profile, the safety/toxicity and the analgesic, anti-inflammatory and antiedematogenic activities of the aqueous extract of the S. agrestis leaves. Eighty individuals were collected at the Nilton Lins University medicinal garden, Manaus, Brazil. The phytochemical profile was obtained through a plant drug survey for cyanogenic heterosides, terpenes, alkaloids and phenolic compounds. The extract safety was evaluated by acute toxicity test. Analgesic and anti-inflammatory activities were accessed using formalin test in mice and the antiedematogenic activity, using paw edema test in mice. We detected phenolic (hydrolysable tannins, coumarins and several classes of flavonoids) and terpenoid (free steroids, saponins) metabolites. We could not establish LD50because no animals died during the acute toxicity test, probably because of the absence of cyanogenic glycosides on the composition of the extract. However, we found that the extract is slightly toxic as animal spasms were observed in the first hour of the test. The extract showed significant analgesic and anti-inflammatory activity on the formalin test (30, 100 and 300 mg/kg p.o.), and the highest dose result was equivalent to the standard drug (Fentanyl). However, no significant antiedematogenic effect was observed during the paw edema test. The results obtained in this study provide preliminary scientific basis about the safety and analgesic/anti-inflammatory actions of the aqueous extract of S. agrestis, which indicates that this species is a promising option for further in vitro and in vivo pharmacological studies. 2020-05-21T21:37:10Z 2020-05-21T21:37:10Z 2014 Artigo https://repositorio.inpa.gov.br/handle/1/16000 10.1590/S1516-05722014000200002 pt_BR Volume 16, Número 2, Pags. 174-181 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Revista Brasileira de Plantas Medicinais |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
pt_BR |
| topic |
Alkaloid Cyanogenic Heteroside Phenol Derivative Plant Extract Plant Medicinal Product Scutellaria Agrestis A Extarct Terpene Unclassified Drug Analgesic Activity Animals Experiment Animals Model Antiedematogenic Activity Antiinflammatory Activity Biological Activity Controlled Study External Otitis Mouse Nonhuman Paw Edema Phytochemistry Scutellaria Toxicity Testing |
| spellingShingle |
Alkaloid Cyanogenic Heteroside Phenol Derivative Plant Extract Plant Medicinal Product Scutellaria Agrestis A Extarct Terpene Unclassified Drug Analgesic Activity Animals Experiment Animals Model Antiedematogenic Activity Antiinflammatory Activity Biological Activity Controlled Study External Otitis Mouse Nonhuman Paw Edema Phytochemistry Scutellaria Toxicity Testing Oliveira, A. B. Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| topic_facet |
Alkaloid Cyanogenic Heteroside Phenol Derivative Plant Extract Plant Medicinal Product Scutellaria Agrestis A Extarct Terpene Unclassified Drug Analgesic Activity Animals Experiment Animals Model Antiedematogenic Activity Antiinflammatory Activity Biological Activity Controlled Study External Otitis Mouse Nonhuman Paw Edema Phytochemistry Scutellaria Toxicity Testing |
| description |
The Scutellaria agrestis is used by Amazonas riverine communities, especially for otitis externa topical treatment, by using the crude extract obtained by maceration. This study aimed to investigate the preliminary phytochemical profile, the safety/toxicity and the analgesic, anti-inflammatory and antiedematogenic activities of the aqueous extract of the S. agrestis leaves. Eighty individuals were collected at the Nilton Lins University medicinal garden, Manaus, Brazil. The phytochemical profile was obtained through a plant drug survey for cyanogenic heterosides, terpenes, alkaloids and phenolic compounds. The extract safety was evaluated by acute toxicity test. Analgesic and anti-inflammatory activities were accessed using formalin test in mice and the antiedematogenic activity, using paw edema test in mice. We detected phenolic (hydrolysable tannins, coumarins and several classes of flavonoids) and terpenoid (free steroids, saponins) metabolites. We could not establish LD50because no animals died during the acute toxicity test, probably because of the absence of cyanogenic glycosides on the composition of the extract. However, we found that the extract is slightly toxic as animal spasms were observed in the first hour of the test. The extract showed significant analgesic and anti-inflammatory activity on the formalin test (30, 100 and 300 mg/kg p.o.), and the highest dose result was equivalent to the standard drug (Fentanyl). However, no significant antiedematogenic effect was observed during the paw edema test. The results obtained in this study provide preliminary scientific basis about the safety and analgesic/anti-inflammatory actions of the aqueous extract of S. agrestis, which indicates that this species is a promising option for further in vitro and in vivo pharmacological studies. |
| format |
Artigo |
| author |
Oliveira, A. B. |
| author2 |
Barbosa, Gleyce dos Santos Verdam, Maria Christina dos Santos Ohana, Débora Teixeira Mendonça, Maria Silvia de Alves Meira, Renata Maria Strozi |
| author2Str |
Barbosa, Gleyce dos Santos Verdam, Maria Christina dos Santos Ohana, Débora Teixeira Mendonça, Maria Silvia de Alves Meira, Renata Maria Strozi |
| title |
Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| title_short |
Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| title_full |
Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| title_fullStr |
Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| title_full_unstemmed |
Analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (Scutellaria agrestis A. St.-Hil. Ex Benth. - Lamiaceae) leaves in rodents |
| title_sort |
analgesic and anti-inflammatory effect of the aqueous extract of "trevo-roxo" (scutellaria agrestis a. st.-hil. ex benth. - lamiaceae) leaves in rodents |
| publisher |
Revista Brasileira de Plantas Medicinais |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16000 |
| _version_ |
1787142592464420864 |
| score |
11.653393 |