Artigo

Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures

The objective of this study was to investigate the effect of temperature on the oxidative metabolism through the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) in the embryonic axis and micropylar endosperm of Melanoxylon brauna Schott seeds during...

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Autor principal: Flôres, Andressa Vasconcelos
Outros Autores: Borges, Eduardo Euclydes de Lima e, Guimarães, Valéria Monteze, Gonçalves, José Francisco Carvalho de, Ataíde, Glauciana da Batista, Barros, Daniela de Pádua
Grau: Artigo
Idioma: pt_BR
Publicado em: Cerne 2020
Assuntos:
Antioxidant
Enzyme Activity
Germination
Legume
Sampling
Temperature Effect
Viability
Melanoxylon Brauna
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/16016
id oai:repositorio:1-16016
recordtype dspace
spelling oai:repositorio:1-16016 Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures Atividade enzimática durante a germinação de sementes de Melanoxylon brauna Schott sob diferentes temperaturas Flôres, Andressa Vasconcelos Borges, Eduardo Euclydes de Lima e Guimarães, Valéria Monteze Gonçalves, José Francisco Carvalho de Ataíde, Glauciana da Batista Barros, Daniela de Pádua Antioxidant Enzyme Activity Germination Legume Sampling Temperature Effect Viability Melanoxylon Brauna The objective of this study was to investigate the effect of temperature on the oxidative metabolism through the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) in the embryonic axis and micropylar endosperm of Melanoxylon brauna Schott seeds during germination. The seeds were sown in paper rolls germitest moistened with distilled water and subjected to constant temperatures of 15, 25, 30 and 40°C for 10 days. Measurements of the activities of enzymes SOD, APX and CAT were made in the seeds germinated under the same conditions described above, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours for the temperature 15°C. There were no activities of the enzymes SOD, APX and CAT in the micropylar endosperm at different temperatures. In the embryonic axis, the activities of the enzymes SOD and CAT showed greater changes over the germination of 15°C and 40°C, demonstrating that temperature stress causes oxidative damage. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low. © 2014, Federal University of Lavras. All rights reserved. 2020-05-21T21:56:30Z 2020-05-21T21:56:30Z 2014 Artigo https://repositorio.inpa.gov.br/handle/1/16016 10.1590/01047760201420031399 pt_BR Volume 20, Número 3, Pags. 401-408 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Cerne
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language pt_BR
topic Antioxidant
Enzyme Activity
Germination
Legume
Sampling
Temperature Effect
Viability
Melanoxylon Brauna
spellingShingle Antioxidant
Enzyme Activity
Germination
Legume
Sampling
Temperature Effect
Viability
Melanoxylon Brauna
Flôres, Andressa Vasconcelos
Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
topic_facet Antioxidant
Enzyme Activity
Germination
Legume
Sampling
Temperature Effect
Viability
Melanoxylon Brauna
description The objective of this study was to investigate the effect of temperature on the oxidative metabolism through the activity of the enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT) in the embryonic axis and micropylar endosperm of Melanoxylon brauna Schott seeds during germination. The seeds were sown in paper rolls germitest moistened with distilled water and subjected to constant temperatures of 15, 25, 30 and 40°C for 10 days. Measurements of the activities of enzymes SOD, APX and CAT were made in the seeds germinated under the same conditions described above, and every 12 hours until the samples were taken root protrusion of 50% of the seeds or 168 hours for the temperature 15°C. There were no activities of the enzymes SOD, APX and CAT in the micropylar endosperm at different temperatures. In the embryonic axis, the activities of the enzymes SOD and CAT showed greater changes over the germination of 15°C and 40°C, demonstrating that temperature stress causes oxidative damage. The APX enzyme activity in the embryonic axis was not affected by temperature during germination and its activity was extremely low. © 2014, Federal University of Lavras. All rights reserved.
format Artigo
author Flôres, Andressa Vasconcelos
author2 Borges, Eduardo Euclydes de Lima e
Guimarães, Valéria Monteze
Gonçalves, José Francisco Carvalho de
Ataíde, Glauciana da Batista
Barros, Daniela de Pádua
author2Str Borges, Eduardo Euclydes de Lima e
Guimarães, Valéria Monteze
Gonçalves, José Francisco Carvalho de
Ataíde, Glauciana da Batista
Barros, Daniela de Pádua
title Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
title_short Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
title_full Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
title_fullStr Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
title_full_unstemmed Enzymatic activity during germination of Melanoxylon brauna schott at different temperatures
title_sort enzymatic activity during germination of melanoxylon brauna schott at different temperatures
publisher Cerne
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/16016
_version_ 1787144702370250752
score 11.755432

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