The Genome of Anopheles darlingi, the main neotropical malaria vector
Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World envir...
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oai:repositorio:1-16049 The Genome of Anopheles darlingi, the main neotropical malaria vector Marinotti, Osvaldo Cerqueira, Gustavo C. Almeida, Luiz G.P. Ferro, Maria Inês Tiraboschi Loreto, Elgion Lúcio da Silva Zaha, Arnaldo Teixeira, Santuza Ribeiro Wespiser, Adam R. e Silva, A. A. Schlindwein, Aline Daiane Pacheco, Ana Carolina Landim Silva, Artur Graveley, Brenton R. Walenz, Brian P. Araújo Lima, Bruna de Ribeiro, Carlos Alexandre Gomes Nunes-Silva, Carlos Gustavo Carvalho, Carlos Roberto de Almeida Soares, Célia Maria A. de Menezes, Cláudia Beatriz Afonso de Matiolli, Cleverson Carlos Caffrey, Daniel R. Araujo, Demetrius Antonio Machado Oliveira, Diana Magalhães de Golenbock, Douglas T. Grisard, Edmundo Carlos Fantinatti-Garboggini, Fabiana Carvalho, Fabíola Marques de Barcellos, Fernando Gomes Prosdocimi, Francisco May, Gemma Azevedo Júnior, Gilson Martins de Guimarães, Giselle Moura Goldman, Gustavo Henrique Padilha, Itácio Q.M. Batista, Jacqueline da Silva Ferro, Jesus Aparecido Ribeiro, J. M.C. Serrao, Jose Eduardo Lopes Rangel Dabbas, Karina Maia Cerdeira, Louise Teixeira Agnez-Lima, Lucymara Fassarella Brocchi, Marcelo Carvalho, Marcos Oliveira de Teixeira, Marcus de Melo Mascena Diniz Maia, Maria de Goldman, Maria Helena S. Schneider, Maria Paula Cruz Felipe, Maria Sueli Soares Hungría, Mariangela Nicolás, Marisa Fabiana Pereira, Maristela Montes, Martín Alejandro Cantão, Maurício Egídio Vincentz, Michel G.A. Rafael, Míriam Silva Silverman, Neal Stoco, Patrícia Hermes Souza, Rangel Celso Vicentini, Renato Gazzinelli, Ricardo Tostes Ostes Oliveira Neves, Rogério de Silva, Rosane A. Astolfi-Filho, Spártaco A.T. Maciel, Talles Eduardo Ferreira Ürményi, Turán Péter Tadei, Wanderli Pedro Camargo, Erney Plessmann Vasconcelos, Ana Tereza Anopheles Anopheles Darlingi Anopheles Gambiae Circadian Rhythm Female Gene Identification Gene Sequence Genetic Conservation Genetic Marker Genome Analysis Genome Size Indel Mutation Insect Genetics Insecticide Resistance Male Nonhuman Priority Journal Sequence Homology Polymorphism, Single Nucleotide Synteny Transposon Xenobiotic Metabolism Animal Anopheles Brasil Chromosomes, Insect Dna Transposable Elements Evolution, Molecular Female Genetic Variation Genome, Insect Host-parasite Interactions Insect Proteins Insect Vectors Insecticide Resistance Insecticides Malaria Male Molecular Sequence Annotation Phylogeny Synteny Transcriptome Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s). 2020-05-22T20:04:32Z 2020-05-22T20:04:32Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/16049 10.1093/nar/gkt484 en Volume 41, Número 15, Pags. 7387-7400 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Nucleic Acids Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
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INPA-RI |
| language |
English |
| topic |
Anopheles Anopheles Darlingi Anopheles Gambiae Circadian Rhythm Female Gene Identification Gene Sequence Genetic Conservation Genetic Marker Genome Analysis Genome Size Indel Mutation Insect Genetics Insecticide Resistance Male Nonhuman Priority Journal Sequence Homology Polymorphism, Single Nucleotide Synteny Transposon Xenobiotic Metabolism Animal Anopheles Brasil Chromosomes, Insect Dna Transposable Elements Evolution, Molecular Female Genetic Variation Genome, Insect Host-parasite Interactions Insect Proteins Insect Vectors Insecticide Resistance Insecticides Malaria Male Molecular Sequence Annotation Phylogeny Synteny Transcriptome |
| spellingShingle |
Anopheles Anopheles Darlingi Anopheles Gambiae Circadian Rhythm Female Gene Identification Gene Sequence Genetic Conservation Genetic Marker Genome Analysis Genome Size Indel Mutation Insect Genetics Insecticide Resistance Male Nonhuman Priority Journal Sequence Homology Polymorphism, Single Nucleotide Synteny Transposon Xenobiotic Metabolism Animal Anopheles Brasil Chromosomes, Insect Dna Transposable Elements Evolution, Molecular Female Genetic Variation Genome, Insect Host-parasite Interactions Insect Proteins Insect Vectors Insecticide Resistance Insecticides Malaria Male Molecular Sequence Annotation Phylogeny Synteny Transcriptome Marinotti, Osvaldo The Genome of Anopheles darlingi, the main neotropical malaria vector |
| topic_facet |
Anopheles Anopheles Darlingi Anopheles Gambiae Circadian Rhythm Female Gene Identification Gene Sequence Genetic Conservation Genetic Marker Genome Analysis Genome Size Indel Mutation Insect Genetics Insecticide Resistance Male Nonhuman Priority Journal Sequence Homology Polymorphism, Single Nucleotide Synteny Transposon Xenobiotic Metabolism Animal Anopheles Brasil Chromosomes, Insect Dna Transposable Elements Evolution, Molecular Female Genetic Variation Genome, Insect Host-parasite Interactions Insect Proteins Insect Vectors Insecticide Resistance Insecticides Malaria Male Molecular Sequence Annotation Phylogeny Synteny Transcriptome |
| description |
Anopheles darlingi is the principal neotropical malaria vector, responsible for more than a million cases of malaria per year on the American continent. Anopheles darlingi diverged from the African and Asian malaria vectors ∼100 million years ago (mya) and successfully adapted to the New World environment. Here we present an annotated reference A. darlingi genome, sequenced from a wild population of males and females collected in the Brazilian Amazon. A total of 10 481 predicted protein-coding genes were annotated, 72% of which have their closest counterpart in Anopheles gambiae and 21% have highest similarity with other mosquito species. In spite of a long period of divergent evolution, conserved gene synteny was observed between A. darlingi and A. gambiae. More than 10 million single nucleotide polymorphisms and short indels with potential use as genetic markers were identified. Transposable elements correspond to 2.3% of the A. darlingi genome. Genes associated with hematophagy, immunity and insecticide resistance, directly involved in vectorhuman and vectorparasite interactions, were identified and discussed. This study represents the first effort to sequence the genome of a neotropical malaria vector, and opens a new window through which we can contemplate the evolutionary history of anopheline mosquitoes. It also provides valuable information that may lead to novel strategies to reduce malaria transmission on the South American continent. The A. darlingi genome is accessible at www.labinfo.lncc.br/index.php/anopheles- darlingi. © 2013 The Author(s). |
| format |
Artigo |
| author |
Marinotti, Osvaldo |
| author2 |
Cerqueira, Gustavo C. Almeida, Luiz G.P. Ferro, Maria Inês Tiraboschi Loreto, Elgion Lúcio da Silva Zaha, Arnaldo Teixeira, Santuza Ribeiro Wespiser, Adam R. e Silva, A. A. Schlindwein, Aline Daiane Pacheco, Ana Carolina Landim Silva, Artur Graveley, Brenton R. Walenz, Brian P. Araújo Lima, Bruna de Ribeiro, Carlos Alexandre Gomes Nunes-Silva, Carlos Gustavo Carvalho, Carlos Roberto de Almeida Soares, Célia Maria A. de Menezes, Cláudia Beatriz Afonso de Matiolli, Cleverson Carlos Caffrey, Daniel R. Araujo, Demetrius Antonio Machado Oliveira, Diana Magalhães de Golenbock, Douglas T. Grisard, Edmundo Carlos Fantinatti-Garboggini, Fabiana Carvalho, Fabíola Marques de Barcellos, Fernando Gomes Prosdocimi, Francisco May, Gemma Azevedo Júnior, Gilson Martins de Guimarães, Giselle Moura Goldman, Gustavo Henrique Padilha, Itácio Q.M. Batista, Jacqueline da Silva Ferro, Jesus Aparecido Ribeiro, J. M.C. Serrao, Jose Eduardo Lopes Rangel Dabbas, Karina Maia Cerdeira, Louise Teixeira Agnez-Lima, Lucymara Fassarella Brocchi, Marcelo Carvalho, Marcos Oliveira de Teixeira, Marcus de Melo Mascena Diniz Maia, Maria de Goldman, Maria Helena S. Schneider, Maria Paula Cruz Felipe, Maria Sueli Soares Hungría, Mariangela Nicolás, Marisa Fabiana Pereira, Maristela Montes, Martín Alejandro Cantão, Maurício Egídio Vincentz, Michel G.A. Rafael, Míriam Silva Silverman, Neal Stoco, Patrícia Hermes Souza, Rangel Celso Vicentini, Renato Gazzinelli, Ricardo Tostes Ostes Oliveira Neves, Rogério de Silva, Rosane A. Astolfi-Filho, Spártaco A.T. Maciel, Talles Eduardo Ferreira Ürményi, Turán Péter Tadei, Wanderli Pedro Camargo, Erney Plessmann Vasconcelos, Ana Tereza |
| author2Str |
Cerqueira, Gustavo C. Almeida, Luiz G.P. Ferro, Maria Inês Tiraboschi Loreto, Elgion Lúcio da Silva Zaha, Arnaldo Teixeira, Santuza Ribeiro Wespiser, Adam R. e Silva, A. A. Schlindwein, Aline Daiane Pacheco, Ana Carolina Landim Silva, Artur Graveley, Brenton R. Walenz, Brian P. Araújo Lima, Bruna de Ribeiro, Carlos Alexandre Gomes Nunes-Silva, Carlos Gustavo Carvalho, Carlos Roberto de Almeida Soares, Célia Maria A. de Menezes, Cláudia Beatriz Afonso de Matiolli, Cleverson Carlos Caffrey, Daniel R. Araujo, Demetrius Antonio Machado Oliveira, Diana Magalhães de Golenbock, Douglas T. Grisard, Edmundo Carlos Fantinatti-Garboggini, Fabiana Carvalho, Fabíola Marques de Barcellos, Fernando Gomes Prosdocimi, Francisco May, Gemma Azevedo Júnior, Gilson Martins de Guimarães, Giselle Moura Goldman, Gustavo Henrique Padilha, Itácio Q.M. Batista, Jacqueline da Silva Ferro, Jesus Aparecido Ribeiro, J. M.C. Serrao, Jose Eduardo Lopes Rangel Dabbas, Karina Maia Cerdeira, Louise Teixeira Agnez-Lima, Lucymara Fassarella Brocchi, Marcelo Carvalho, Marcos Oliveira de Teixeira, Marcus de Melo Mascena Diniz Maia, Maria de Goldman, Maria Helena S. Schneider, Maria Paula Cruz Felipe, Maria Sueli Soares Hungría, Mariangela Nicolás, Marisa Fabiana Pereira, Maristela Montes, Martín Alejandro Cantão, Maurício Egídio Vincentz, Michel G.A. Rafael, Míriam Silva Silverman, Neal Stoco, Patrícia Hermes Souza, Rangel Celso Vicentini, Renato Gazzinelli, Ricardo Tostes Ostes Oliveira Neves, Rogério de Silva, Rosane A. Astolfi-Filho, Spártaco A.T. Maciel, Talles Eduardo Ferreira Ürményi, Turán Péter Tadei, Wanderli Pedro Camargo, Erney Plessmann Vasconcelos, Ana Tereza |
| title |
The Genome of Anopheles darlingi, the main neotropical malaria vector |
| title_short |
The Genome of Anopheles darlingi, the main neotropical malaria vector |
| title_full |
The Genome of Anopheles darlingi, the main neotropical malaria vector |
| title_fullStr |
The Genome of Anopheles darlingi, the main neotropical malaria vector |
| title_full_unstemmed |
The Genome of Anopheles darlingi, the main neotropical malaria vector |
| title_sort |
genome of anopheles darlingi, the main neotropical malaria vector |
| publisher |
Nucleic Acids Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16049 |
| _version_ |
1787144702816944128 |
| score |
11.755432 |