Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey
Background: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless b...
| Autor principal: | Pimentel, Renah Boanerges de Queiroz |
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| Outros Autores: | Costa, Cristóvão Alves da, Albuquerque, Patrícia Melchionna, Duvoisin Júnior, Sérgio |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
BMC Complementary and Alternative Medicine
2020
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https://repositorio.inpa.gov.br/handle/1/16056 |
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oai:repositorio:1-16056 |
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oai:repositorio:1-16056 Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey Pimentel, Renah Boanerges de Queiroz Costa, Cristóvão Alves da Albuquerque, Patrícia Melchionna Duvoisin Júnior, Sérgio Agar Apigenin Flavonoid Phenol Derivative Rutoside Agar Diffusion Antibacterial Activity Apis Bacterial Growth Broth Dilution Chromatography Controlled Study Entomopathogenic Bacterium Escherichia Coli Gram Negative Bacterium Gram Positive Bacterium High Performance Liquid Chromatography Honey Klebsiella Liquid Liquid Extraction Nonhuman Proteus Vulgaris Salmonella Paratyphi Season Shigella Sonnei Staphylococcus Aureus Stingless Bee Animal Anti-bacterial Agents Apitherapy Bees Chromatography, High Pressure Liquid Gram-negative Bacteria Gram-positive Bacteria Honey Phenols Rutin Background: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.Methods: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.Results: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.Conclusions: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth. © 2013 Pimentel et al.; licensee BioMed Central Ltd. 2020-05-22T20:04:34Z 2020-05-22T20:04:34Z 2013 Artigo https://repositorio.inpa.gov.br/handle/1/16056 10.1186/1472-6882-13-151 en Volume 13 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf BMC Complementary and Alternative Medicine |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Agar Apigenin Flavonoid Phenol Derivative Rutoside Agar Diffusion Antibacterial Activity Apis Bacterial Growth Broth Dilution Chromatography Controlled Study Entomopathogenic Bacterium Escherichia Coli Gram Negative Bacterium Gram Positive Bacterium High Performance Liquid Chromatography Honey Klebsiella Liquid Liquid Extraction Nonhuman Proteus Vulgaris Salmonella Paratyphi Season Shigella Sonnei Staphylococcus Aureus Stingless Bee Animal Anti-bacterial Agents Apitherapy Bees Chromatography, High Pressure Liquid Gram-negative Bacteria Gram-positive Bacteria Honey Phenols Rutin |
| spellingShingle |
Agar Apigenin Flavonoid Phenol Derivative Rutoside Agar Diffusion Antibacterial Activity Apis Bacterial Growth Broth Dilution Chromatography Controlled Study Entomopathogenic Bacterium Escherichia Coli Gram Negative Bacterium Gram Positive Bacterium High Performance Liquid Chromatography Honey Klebsiella Liquid Liquid Extraction Nonhuman Proteus Vulgaris Salmonella Paratyphi Season Shigella Sonnei Staphylococcus Aureus Stingless Bee Animal Anti-bacterial Agents Apitherapy Bees Chromatography, High Pressure Liquid Gram-negative Bacteria Gram-positive Bacteria Honey Phenols Rutin Pimentel, Renah Boanerges de Queiroz Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| topic_facet |
Agar Apigenin Flavonoid Phenol Derivative Rutoside Agar Diffusion Antibacterial Activity Apis Bacterial Growth Broth Dilution Chromatography Controlled Study Entomopathogenic Bacterium Escherichia Coli Gram Negative Bacterium Gram Positive Bacterium High Performance Liquid Chromatography Honey Klebsiella Liquid Liquid Extraction Nonhuman Proteus Vulgaris Salmonella Paratyphi Season Shigella Sonnei Staphylococcus Aureus Stingless Bee Animal Anti-bacterial Agents Apitherapy Bees Chromatography, High Pressure Liquid Gram-negative Bacteria Gram-positive Bacteria Honey Phenols Rutin |
| description |
Background: Honey has been identified as a potential alternative to the widespread use of antibiotics, which are of significant concern considering the emergence of resistant bacteria. In this context, this study aimed to evaluate the antimicrobial activity of honey samples produced by a stingless bee species and by Apis sp. against pathogenic bacteria, as well as to identify the presence of phenolic compounds.Methods: Honey samples from the stingless bee M. compressipes manaosensis were collected twice, during the dry and rainy seasons. Three commercial honey samples from Apis sp. were also included in this study. Two different assays were performed to evaluate the antibacterial potential of the honey samples: agar-well diffusion and broth macrodilution. Liquid-liquid extraction was used to assess phenolic compounds from honey. HPLC analysis was performed in order to identify rutin and apigenin on honey samples. Chromatograms were recorded at 340 and 290 nm.Results: Two honey samples were identified as having the highest antimicrobial activity using the agar diffusion method. Honey produced by Melipona compressipes manaosensis inhibited the growth of Staphylococcus aureus, Escherichia coli (0157: H7), Proteus vulgaris, Shigella sonnei and Klebsiella sp. A sample of honey produced by Apis sp. also inhibited the growth of Salmonella paratyphi. The macrodilution technique presented greater sensitivity for the antibacterial testing, since all honey samples showed activity. Flavonoid rutin was identified in the honey sample produced by the stingless bee.Conclusions: Honey samples tested in this work showed antibacterial activity against Gram-positive and Gram-negative bacteria. The results reported herein highlight the potential of using honey to control bacterial growth. © 2013 Pimentel et al.; licensee BioMed Central Ltd. |
| format |
Artigo |
| author |
Pimentel, Renah Boanerges de Queiroz |
| author2 |
Costa, Cristóvão Alves da Albuquerque, Patrícia Melchionna Duvoisin Júnior, Sérgio |
| author2Str |
Costa, Cristóvão Alves da Albuquerque, Patrícia Melchionna Duvoisin Júnior, Sérgio |
| title |
Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| title_short |
Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| title_full |
Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| title_fullStr |
Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| title_full_unstemmed |
Antimicrobial activity and rutin identification of honey produced by the stingless bee Melipona compressipes manaosensis and commercial honey |
| title_sort |
antimicrobial activity and rutin identification of honey produced by the stingless bee melipona compressipes manaosensis and commercial honey |
| publisher |
BMC Complementary and Alternative Medicine |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16056 |
| _version_ |
1787141312053510144 |
| score |
11.755432 |