Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA
Our group analyzed species of Anopheles of the subgenus Nyssorhynchus (An. darlingi, An. marajoara, An. oswaldoi, An. benarrochi, An. triannulatus and An. rangeli) and a single species of the subgenus Anopheles (An. mattogrossensis), originating in the Amazon, based on the mitochondrial DNA control...
| Autor principal: | Borges-Moroni, R. |
|---|---|
| Outros Autores: | Fraga, Elmary da Costa, Maia, Juracy de Freitas, Tadei, Wanderli Pedro, Santos, Joselita Maria M. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Open Tropical Medicine Journal
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16132 |
| id |
oai:repositorio:1-16132 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-16132 Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA Borges-Moroni, R. Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro Santos, Joselita Maria M. Dna, Mitochondrial Anopheles Anopheles Benarrochi Anopheles Darlingi Anopheles Marajoara Anopheles Mattogrossensis Anopheles Oswaldoi Anopheles Rangeli Anopheles Triannulatus Cellia Controlled Study Dna Polymorphism Gene Mutation Genetic Distance Genetic Variability Genus Haplotype Nonhuman Nucleic Acid Base Substitution Nyssorhychus Phylogeny Polymorphic Locus Priority Journal Species Difference Species Identification Our group analyzed species of Anopheles of the subgenus Nyssorhynchus (An. darlingi, An. marajoara, An. oswaldoi, An. benarrochi, An. triannulatus and An. rangeli) and a single species of the subgenus Anopheles (An. mattogrossensis), originating in the Amazon, based on the mitochondrial DNA control region. The fragment had 381 pb and the nucleotide composition in A+T ranged from 85.8 to 89.5%. DNA polymorphism analysis of the species detected 15 haplotypes, with the presence of 81 polymorphic loci, 95 mutations, haplotypic divergence of 0.879, nucleotide diversity of 0.06507, nucleotide differences pair the pair mean of 23.1749, and the observed and expected variances of 113.840 and 8.33, respectively. The genetic distance among the Anopheles species varied from 0.06 to 1.37%. An. oswaldoi and An. rangeli were the most similar, with nucleotide divergence of 0.17%. The An. benarrochi populations of Ji-Paraná and Bolivia showed nucleotide divergence of 0.06%. An. darlingi, An. marajoara and An. triannulatus presented nucleotide divergences of 0.35% between An. triannulatus and An. darlingiand of 0.47% between An. darlingi and An. marajoara. This data showed ample interspecific nucleotide variation, though with low nucleotide divergence. The cladogram separated the species of the subgenus Nyssorhynchus and those of the subgenera Cellia and Anopheles, with 98% bootstrap. The region control data did not show strong phylogenetic support, as indicated by the transition/transversion mean rate (0.4643), which is necessary for increasing fragment size and using other more conservative genes for greater inference concerning the phylogeny of the Anopheles species of the subgenus Nyssorhynchus. © Borges-Moroni et al. 2020-05-24T22:15:12Z 2020-05-24T22:15:12Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/16132 10.2174/1874315301205010006 en Volume 5, Número 1, Pags. 6-11 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Open Tropical Medicine Journal |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna, Mitochondrial Anopheles Anopheles Benarrochi Anopheles Darlingi Anopheles Marajoara Anopheles Mattogrossensis Anopheles Oswaldoi Anopheles Rangeli Anopheles Triannulatus Cellia Controlled Study Dna Polymorphism Gene Mutation Genetic Distance Genetic Variability Genus Haplotype Nonhuman Nucleic Acid Base Substitution Nyssorhychus Phylogeny Polymorphic Locus Priority Journal Species Difference Species Identification |
| spellingShingle |
Dna, Mitochondrial Anopheles Anopheles Benarrochi Anopheles Darlingi Anopheles Marajoara Anopheles Mattogrossensis Anopheles Oswaldoi Anopheles Rangeli Anopheles Triannulatus Cellia Controlled Study Dna Polymorphism Gene Mutation Genetic Distance Genetic Variability Genus Haplotype Nonhuman Nucleic Acid Base Substitution Nyssorhychus Phylogeny Polymorphic Locus Priority Journal Species Difference Species Identification Borges-Moroni, R. Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| topic_facet |
Dna, Mitochondrial Anopheles Anopheles Benarrochi Anopheles Darlingi Anopheles Marajoara Anopheles Mattogrossensis Anopheles Oswaldoi Anopheles Rangeli Anopheles Triannulatus Cellia Controlled Study Dna Polymorphism Gene Mutation Genetic Distance Genetic Variability Genus Haplotype Nonhuman Nucleic Acid Base Substitution Nyssorhychus Phylogeny Polymorphic Locus Priority Journal Species Difference Species Identification |
| description |
Our group analyzed species of Anopheles of the subgenus Nyssorhynchus (An. darlingi, An. marajoara, An. oswaldoi, An. benarrochi, An. triannulatus and An. rangeli) and a single species of the subgenus Anopheles (An. mattogrossensis), originating in the Amazon, based on the mitochondrial DNA control region. The fragment had 381 pb and the nucleotide composition in A+T ranged from 85.8 to 89.5%. DNA polymorphism analysis of the species detected 15 haplotypes, with the presence of 81 polymorphic loci, 95 mutations, haplotypic divergence of 0.879, nucleotide diversity of 0.06507, nucleotide differences pair the pair mean of 23.1749, and the observed and expected variances of 113.840 and 8.33, respectively. The genetic distance among the Anopheles species varied from 0.06 to 1.37%. An. oswaldoi and An. rangeli were the most similar, with nucleotide divergence of 0.17%. The An. benarrochi populations of Ji-Paraná and Bolivia showed nucleotide divergence of 0.06%. An. darlingi, An. marajoara and An. triannulatus presented nucleotide divergences of 0.35% between An. triannulatus and An. darlingiand of 0.47% between An. darlingi and An. marajoara. This data showed ample interspecific nucleotide variation, though with low nucleotide divergence. The cladogram separated the species of the subgenus Nyssorhynchus and those of the subgenera Cellia and Anopheles, with 98% bootstrap. The region control data did not show strong phylogenetic support, as indicated by the transition/transversion mean rate (0.4643), which is necessary for increasing fragment size and using other more conservative genes for greater inference concerning the phylogeny of the Anopheles species of the subgenus Nyssorhynchus. © Borges-Moroni et al. |
| format |
Artigo |
| author |
Borges-Moroni, R. |
| author2 |
Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro Santos, Joselita Maria M. |
| author2Str |
Fraga, Elmary da Costa Maia, Juracy de Freitas Tadei, Wanderli Pedro Santos, Joselita Maria M. |
| title |
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| title_short |
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| title_full |
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| title_fullStr |
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| title_full_unstemmed |
Genetic differentiation in species of Anopheles from the subgenera Nyssorhynchus based on mitochondrial DNA |
| title_sort |
genetic differentiation in species of anopheles from the subgenera nyssorhynchus based on mitochondrial dna |
| publisher |
Open Tropical Medicine Journal |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16132 |
| _version_ |
1787143549264855040 |
| score |
11.755432 |