Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related prot...
| Autor principal: | Oliveira, Sabrina Alberti Nóbrega de |
|---|---|
| Outros Autores: | Ibraim, Izabela Coimbra, Tadei, Wanderli Pedro, Ruiz, Jerônimo Conceição, Nahum, Laila Alves, Brito, Cristiana Ferreira Alves de, Moreira, Luciano Andrade |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Malaria Journal
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16211 |
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oai:repositorio:1-16211 |
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oai:repositorio:1-16211 Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes Oliveira, Sabrina Alberti Nóbrega de Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd. 2020-05-31T18:24:28Z 2020-05-31T18:24:28Z 2011 Artigo https://repositorio.inpa.gov.br/handle/1/16211 10.1186/1475-2875-10-21 en Volume 10 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Malaria Journal |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis |
| spellingShingle |
Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis Oliveira, Sabrina Alberti Nóbrega de Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| topic_facet |
Amino Acid Fibrinogen Related Protein 9 Insect Proteins Nucleotide Unclassified Drug Fibrinogen Immunoglobulin Aedes Aegypti Amino Acid Substitution Anopheles Anopheles Albitarsis Anopheles Aquasalis Anopheles Darlingi Anopheles Marajoara Anopheles Merus Anopheles Nuneztovari Anopheles Quadriannulatus Bayes Theorem Cladistics Comparative Study Controlled Study Disease Carrier Evolutionary Homology Gene Identification Gene Sequence Genetic Variability Genotype Cloning, Molecular Neotropics Nonhuman Nucleotide Sequence Phylogeny Sequence Homology Polymorphism, Single Nucleotide Amino Acid Sequence Animals Brasil Classification Gene Genetics Immunology Malaria Evolution, Molecular Parasitology Sequence Analysis Amino Acid Sequence Animal Anopheles Base Sequence Brasil Cloning, Molecular Evolution, Molecular Fibrinogen Genes, Insect Immunoglobulins Insect Vectors Malaria Phylogeny Sequence Analysis |
| description |
Background: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. Methods. This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. Results: Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. Conclusions: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies. © 2011 Oliveira et al; licensee BioMed Central Ltd. |
| format |
Artigo |
| author |
Oliveira, Sabrina Alberti Nóbrega de |
| author2 |
Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade |
| author2Str |
Ibraim, Izabela Coimbra Tadei, Wanderli Pedro Ruiz, Jerônimo Conceição Nahum, Laila Alves Brito, Cristiana Ferreira Alves de Moreira, Luciano Andrade |
| title |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| title_short |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| title_full |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| title_fullStr |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| title_full_unstemmed |
Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes |
| title_sort |
identification of a fibrinogen-related protein (fbn9) gene in neotropical anopheline mosquitoes |
| publisher |
Malaria Journal |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16211 |
| _version_ |
1787143175893155840 |
| score |
11.755432 |