Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains
Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 °C for R...
| Autor principal: | Oliveira, Arlem Nascimento de |
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| Outros Autores: | Oliveira, Luiz Antonio de, Andrade, Jerusa Souza |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Brazilian Archives of Biology and Technology
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16247 |
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oai:repositorio:1-16247 |
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dspace |
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oai:repositorio:1-16247 Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains Oliveira, Arlem Nascimento de Oliveira, Luiz Antonio de Andrade, Jerusa Souza Bradyrhizobium Bradyrhizobium Sp. Rhizobiaceae Rhizobium Rhizobium Sp. Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 °C for Rhizobium sp. strain R-986 and 55 °C for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene). 2020-06-02T15:09:53Z 2020-06-02T15:09:53Z 2010 Artigo https://repositorio.inpa.gov.br/handle/1/16247 10.1590/S1516-89132010000500001 en Volume 53, Número 5, Pags. 1185-1195 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Brazilian Archives of Biology and Technology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Bradyrhizobium Bradyrhizobium Sp. Rhizobiaceae Rhizobium Rhizobium Sp. |
| spellingShingle |
Bradyrhizobium Bradyrhizobium Sp. Rhizobiaceae Rhizobium Rhizobium Sp. Oliveira, Arlem Nascimento de Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| topic_facet |
Bradyrhizobium Bradyrhizobium Sp. Rhizobiaceae Rhizobium Rhizobium Sp. |
| description |
Two rhizobia strains isolated from soils of the Central Amazonian floodplain produced appreciable quantities of crude alkaline protease extracts with inexpensive carbon and nitrogen sources. These protease crude extracts were optimally active at pH 9.0-11.0. The optimum temperatures were 35 °C for Rhizobium sp. strain R-986 and 55 °C for Bradyrhizobium sp. strain R-993. Protease activities in the crude extracts were enhanced in the presence of 5 mM metal ions, such as Na+, Ca2+, Mg2+ and Mn2+. Rhizobia proteases were strongly inhibited by PMSF, a serine-protease inhibitor. The enzymes were active in the presence of surfactants (SDS and Triton X-100) and stable in oxidizing (H2O2) and reducing agents (β-mercaptoethanol), and organic solvents (acetone, hexane, methanol, 1-propanol and toluene). |
| format |
Artigo |
| author |
Oliveira, Arlem Nascimento de |
| author2 |
Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
| author2Str |
Oliveira, Luiz Antonio de Andrade, Jerusa Souza |
| title |
Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_short |
Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_full |
Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_fullStr |
Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_full_unstemmed |
Production and some properties of crude alkaline proteases of indigenous Central Amazonian rhizobia strains |
| title_sort |
production and some properties of crude alkaline proteases of indigenous central amazonian rhizobia strains |
| publisher |
Brazilian Archives of Biology and Technology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16247 |
| _version_ |
1787142755159375872 |
| score |
11.755432 |