Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display
In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due...
| Autor principal: | Siquieroli, Ana Carolina Silva |
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| Outros Autores: | Vieira, Carlos Ueira, Carvalho-Zelse, Gislene A., Goulart, L. R., Kerr, Warwick Estevam, Bonetti, Ana Maria |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Biological Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16314 |
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oai:repositorio:1-16314 |
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oai:repositorio:1-16314 Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display Siquieroli, Ana Carolina Silva Vieira, Carlos Ueira Carvalho-Zelse, Gislene A. Goulart, L. R. Kerr, Warwick Estevam Bonetti, Ana Maria Rna, Messenger Abdominal Ganglion Cell Count Controlled Study Gene Expression Profiling Genetic Transcription Genetic Variability Insect Caste Melipona Scutellaris Nonhuman Pupa (life Cycle Stage) Queen (insect) Reverse Transcription Polymerase Chain Reaction Rna Analysis Species Comparison Stingless Bee Worker (insect) Apidae Hymenoptera Melipona Melipona Scutellaris Meliponinae Zosteropidae In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers. 2020-06-03T19:27:21Z 2020-06-03T19:27:21Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/16314 10.4067/S0716-97602009000100011 en Volume 42, Número 1, Pags. 107-110 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Biological Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Rna, Messenger Abdominal Ganglion Cell Count Controlled Study Gene Expression Profiling Genetic Transcription Genetic Variability Insect Caste Melipona Scutellaris Nonhuman Pupa (life Cycle Stage) Queen (insect) Reverse Transcription Polymerase Chain Reaction Rna Analysis Species Comparison Stingless Bee Worker (insect) Apidae Hymenoptera Melipona Melipona Scutellaris Meliponinae Zosteropidae |
| spellingShingle |
Rna, Messenger Abdominal Ganglion Cell Count Controlled Study Gene Expression Profiling Genetic Transcription Genetic Variability Insect Caste Melipona Scutellaris Nonhuman Pupa (life Cycle Stage) Queen (insect) Reverse Transcription Polymerase Chain Reaction Rna Analysis Species Comparison Stingless Bee Worker (insect) Apidae Hymenoptera Melipona Melipona Scutellaris Meliponinae Zosteropidae Siquieroli, Ana Carolina Silva Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| topic_facet |
Rna, Messenger Abdominal Ganglion Cell Count Controlled Study Gene Expression Profiling Genetic Transcription Genetic Variability Insect Caste Melipona Scutellaris Nonhuman Pupa (life Cycle Stage) Queen (insect) Reverse Transcription Polymerase Chain Reaction Rna Analysis Species Comparison Stingless Bee Worker (insect) Apidae Hymenoptera Melipona Melipona Scutellaris Meliponinae Zosteropidae |
| description |
In colonies of Melipona scutellaris Latreille, 1811 workers can be found with four ganglion nerve cells, a morphological characteristic of the queen. It is hypothesized that these workers, called intercastes, or phenocopies, are phenotypically-like workers, but genotypically identical to queens due to this specific trait. Workers with the same number of ganglion as queens seem to be intercastes between queens and workers. Our objective was to analyze the mRNA profiles of workers, queens, and intercastes of M. scutellaris through DDRT-PCR. Three hundred (300) pupae with white eyes were collected and externally identified according to the number of abdominal nerve ganglions: workers (5 ganglions), queens (4 ganglions) and intercastes (4 ganglions). The analysis identified differentially expressed transcripts that were present only in workers, but absent in intercastes and queens, confirming the hypothesis, by demonstrating the environmental effect on the queen genotype that generated phenotype-like workers. |
| format |
Artigo |
| author |
Siquieroli, Ana Carolina Silva |
| author2 |
Vieira, Carlos Ueira Carvalho-Zelse, Gislene A. Goulart, L. R. Kerr, Warwick Estevam Bonetti, Ana Maria |
| author2Str |
Vieira, Carlos Ueira Carvalho-Zelse, Gislene A. Goulart, L. R. Kerr, Warwick Estevam Bonetti, Ana Maria |
| title |
Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| title_short |
Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| title_full |
Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| title_fullStr |
Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| title_full_unstemmed |
Analysis of the intercaste transcriptional profile of Melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mRNA differential display |
| title_sort |
analysis of the intercaste transcriptional profile of melipona scutellaris latreille, 1811 (hymenoptera, apidae, meliponini) by mrna differential display |
| publisher |
Biological Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16314 |
| _version_ |
1787142942108942336 |
| score |
11.755432 |