Artigo

Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state

Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI se...

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Autor principal: Barros, Regina Maria de Souza
Outros Autores: Pieczarka, Júlio César, Nagamachi, Cleusa Yoshiko, Feldberg, Eliana, Nascimento, Aline L, Cesar, Augusto C.G., Carvalho, Jaime Ribeiro
Grau: Artigo
Idioma: English
Publicado em: Caryologia 2020
Assuntos:
Cichlidae
Perciformes
Pterophyllum Scalare
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/16374
id oai:repositorio:1-16374
recordtype dspace
spelling oai:repositorio:1-16374 Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state Barros, Regina Maria de Souza Pieczarka, Júlio César Nagamachi, Cleusa Yoshiko Feldberg, Eliana Nascimento, Aline L Cesar, Augusto C.G. Carvalho, Jaime Ribeiro Cichlidae Perciformes Pterophyllum Scalare Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining and fluorochrome in situ hybridization with human telomere probes. All individuals showed a chromosome number of 2n = 48 (12 M/SM and 36 ST/A) and FN = 60. No differences were detected between male and female karyotypes, indicating the absence of morphologically differentiated mitotic sexual chromosomes. Constitutive heterochromatin blocks were located at the centromeric and pericentromeric regions of all chromosomes. The largest submetacentric pair showed a differential staining on their short arms. Only two NOR bearing chromosomes were detected, and the stainings were observed at the distal region of the short arm of the largest chromosome pair, matching the secondary constriction. Chromomycin A3, stained the NOR and the centromeres of some chromosomes. DAPI-bands were observed at the centromeric regions of all chromosomes. Telomere sequences hybridised only at the terminal regions. © 2006 Taylor and Francis Group, LLC. 2020-06-04T13:50:32Z 2020-06-04T13:50:32Z 2006 Artigo https://repositorio.inpa.gov.br/handle/1/16374 10.1080/00087114.2006.10797909 en Volume 59, Número 2, Pags. 138-143 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Caryologia
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
topic Cichlidae
Perciformes
Pterophyllum Scalare
spellingShingle Cichlidae
Perciformes
Pterophyllum Scalare
Barros, Regina Maria de Souza
Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
topic_facet Cichlidae
Perciformes
Pterophyllum Scalare
description Cytogenetic studies were carried out on eighteen wild specimens of Pterophyllum scalare from Jari River, in Pará state, and the results were compared to literature. Mitotic chromosomes were obtained from kidney cells and the analysis was done using: C-banding, Ag-NOR staining, Chromomycin A3/DAPI sequential staining and fluorochrome in situ hybridization with human telomere probes. All individuals showed a chromosome number of 2n = 48 (12 M/SM and 36 ST/A) and FN = 60. No differences were detected between male and female karyotypes, indicating the absence of morphologically differentiated mitotic sexual chromosomes. Constitutive heterochromatin blocks were located at the centromeric and pericentromeric regions of all chromosomes. The largest submetacentric pair showed a differential staining on their short arms. Only two NOR bearing chromosomes were detected, and the stainings were observed at the distal region of the short arm of the largest chromosome pair, matching the secondary constriction. Chromomycin A3, stained the NOR and the centromeres of some chromosomes. DAPI-bands were observed at the centromeric regions of all chromosomes. Telomere sequences hybridised only at the terminal regions. © 2006 Taylor and Francis Group, LLC.
format Artigo
author Barros, Regina Maria de Souza
author2 Pieczarka, Júlio César
Nagamachi, Cleusa Yoshiko
Feldberg, Eliana
Nascimento, Aline L
Cesar, Augusto C.G.
Carvalho, Jaime Ribeiro
author2Str Pieczarka, Júlio César
Nagamachi, Cleusa Yoshiko
Feldberg, Eliana
Nascimento, Aline L
Cesar, Augusto C.G.
Carvalho, Jaime Ribeiro
title Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
title_short Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
title_full Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
title_fullStr Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
title_full_unstemmed Cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
title_sort cytogenetic analysis on pterophyllum scalare (perciformes, cichlidae) from jari river, pará state
publisher Caryologia
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/16374
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score 11.755432

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