Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil
Numerical zymotaxonomy and variability of the internal transcribed spacers (ITS) between the small and large subunits of the rRNA genes were used to examine strain variation and relationships in natural populations of Leishmania (Viannia) braziliensis. A total of 101 strains from distinct hosts and...
| Autor principal: | Cupolillo, Elisa |
|---|---|
| Outros Autores: | Brahim, Lúcia Regina, Toaldo, Cristiane Bentin, Paes de Oliveira-Neto, Manoel, Brito, Maria Edileuza Felinto de, Falqueto, Aloi?io, Naiff, Maricleide de Farias, Grimaldi Júnior, Gabriel F. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Journal of Clinical Microbiology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16403 |
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oai:repositorio:1-16403 |
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oai:repositorio:1-16403 Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil Cupolillo, Elisa Brahim, Lúcia Regina Toaldo, Cristiane Bentin Paes de Oliveira-Neto, Manoel Brito, Maria Edileuza Felinto de Falqueto, Aloi?io Naiff, Maricleide de Farias Grimaldi Júnior, Gabriel F. Controlled Study Epidemiological Data Genetic Epidemiology Polymorphism, Genetic Genotype Geographic Distribution Geographic Pathology Host Parasite Interaction Leishmania Braziliensis Microbial Diversity Nonhuman Parasite Vector Polymerase Chain Reaction Priority Journal Psychodidae Polymorphism, Restriction Fragment Length Species Endemicity Strain Identification Taxonomy Animal Brasil Dna, Protozoan Dna, Ribosomal Spacer Electrophoresis Epidemiology, Molecular Genes, Rrna Host-parasite Relations Humans Leishmania Braziliensis Leishmaniasis, Cutaneous Polymorphism, Genetic Polymorphism, Restriction Fragment Length Leishmania Braziliensis Protozoa Psychodidae Numerical zymotaxonomy and variability of the internal transcribed spacers (ITS) between the small and large subunits of the rRNA genes were used to examine strain variation and relationships in natural populations of Leishmania (Viannia) braziliensis. A total of 101 strains from distinct hosts and Brazilian geographic regions were assigned to 15 zymodemes clustered in two major genetic groups. The great number of isolates (48.5%) placed in zymodeme IOC/Z-27 were collected on the Atlantic coast. The high molecular diversity found in populations in the Amazon Basin was related to the great number of sandfly vector(s) in that region. The results of the restriction fragment length polymorphism analysis of the ITS depicted considerable intraspecific variation. Genotypic groups A, B, and C contained 39, 40, and 22 isolates, which were divided into 16, 10, and 15 genotypes, respectively. The genetic polymorphism observed demonstrates the degree of diversity of L. (V.) braziliensis strains from different regions where they are endemic. The results reinforce the clonal theory for Leishmania parasites showing the genetic diversity of this pathogen and an association of L. (V.) braziliensis genotypes with specific transmission cycles, probably reflecting an adaptation of different clones to the vector species involved. 2020-06-04T15:17:40Z 2020-06-04T15:17:40Z 2003 Artigo https://repositorio.inpa.gov.br/handle/1/16403 10.1128/JCM.41.7.3126-3132.2003 en Volume 41, Número 7, Pags. 3126-3132 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf Journal of Clinical Microbiology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Controlled Study Epidemiological Data Genetic Epidemiology Polymorphism, Genetic Genotype Geographic Distribution Geographic Pathology Host Parasite Interaction Leishmania Braziliensis Microbial Diversity Nonhuman Parasite Vector Polymerase Chain Reaction Priority Journal Psychodidae Polymorphism, Restriction Fragment Length Species Endemicity Strain Identification Taxonomy Animal Brasil Dna, Protozoan Dna, Ribosomal Spacer Electrophoresis Epidemiology, Molecular Genes, Rrna Host-parasite Relations Humans Leishmania Braziliensis Leishmaniasis, Cutaneous Polymorphism, Genetic Polymorphism, Restriction Fragment Length Leishmania Braziliensis Protozoa Psychodidae |
| spellingShingle |
Controlled Study Epidemiological Data Genetic Epidemiology Polymorphism, Genetic Genotype Geographic Distribution Geographic Pathology Host Parasite Interaction Leishmania Braziliensis Microbial Diversity Nonhuman Parasite Vector Polymerase Chain Reaction Priority Journal Psychodidae Polymorphism, Restriction Fragment Length Species Endemicity Strain Identification Taxonomy Animal Brasil Dna, Protozoan Dna, Ribosomal Spacer Electrophoresis Epidemiology, Molecular Genes, Rrna Host-parasite Relations Humans Leishmania Braziliensis Leishmaniasis, Cutaneous Polymorphism, Genetic Polymorphism, Restriction Fragment Length Leishmania Braziliensis Protozoa Psychodidae Cupolillo, Elisa Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| topic_facet |
Controlled Study Epidemiological Data Genetic Epidemiology Polymorphism, Genetic Genotype Geographic Distribution Geographic Pathology Host Parasite Interaction Leishmania Braziliensis Microbial Diversity Nonhuman Parasite Vector Polymerase Chain Reaction Priority Journal Psychodidae Polymorphism, Restriction Fragment Length Species Endemicity Strain Identification Taxonomy Animal Brasil Dna, Protozoan Dna, Ribosomal Spacer Electrophoresis Epidemiology, Molecular Genes, Rrna Host-parasite Relations Humans Leishmania Braziliensis Leishmaniasis, Cutaneous Polymorphism, Genetic Polymorphism, Restriction Fragment Length Leishmania Braziliensis Protozoa Psychodidae |
| description |
Numerical zymotaxonomy and variability of the internal transcribed spacers (ITS) between the small and large subunits of the rRNA genes were used to examine strain variation and relationships in natural populations of Leishmania (Viannia) braziliensis. A total of 101 strains from distinct hosts and Brazilian geographic regions were assigned to 15 zymodemes clustered in two major genetic groups. The great number of isolates (48.5%) placed in zymodeme IOC/Z-27 were collected on the Atlantic coast. The high molecular diversity found in populations in the Amazon Basin was related to the great number of sandfly vector(s) in that region. The results of the restriction fragment length polymorphism analysis of the ITS depicted considerable intraspecific variation. Genotypic groups A, B, and C contained 39, 40, and 22 isolates, which were divided into 16, 10, and 15 genotypes, respectively. The genetic polymorphism observed demonstrates the degree of diversity of L. (V.) braziliensis strains from different regions where they are endemic. The results reinforce the clonal theory for Leishmania parasites showing the genetic diversity of this pathogen and an association of L. (V.) braziliensis genotypes with specific transmission cycles, probably reflecting an adaptation of different clones to the vector species involved. |
| format |
Artigo |
| author |
Cupolillo, Elisa |
| author2 |
Brahim, Lúcia Regina Toaldo, Cristiane Bentin Paes de Oliveira-Neto, Manoel Brito, Maria Edileuza Felinto de Falqueto, Aloi?io Naiff, Maricleide de Farias Grimaldi Júnior, Gabriel F. |
| author2Str |
Brahim, Lúcia Regina Toaldo, Cristiane Bentin Paes de Oliveira-Neto, Manoel Brito, Maria Edileuza Felinto de Falqueto, Aloi?io Naiff, Maricleide de Farias Grimaldi Júnior, Gabriel F. |
| title |
Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| title_short |
Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| title_full |
Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| title_fullStr |
Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| title_full_unstemmed |
Genetic polymorphism and molecular epidemiology of Leishmania (Viannia) braziliensis from different hosts and geographic areas in Brazil |
| title_sort |
genetic polymorphism and molecular epidemiology of leishmania (viannia) braziliensis from different hosts and geographic areas in brazil |
| publisher |
Journal of Clinical Microbiology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16403 |
| _version_ |
1787141478649167872 |
| score |
11.755432 |