Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences
Traditional molecular and biochemical methods, such as schizodeme analysis, karyotyping, DNA fingerprinting, and enzyme electrophoretic profiles, have shown a large variability among Trypanosoma cruzi isolates. In contrast to those results, polymerase chain reaction (PCR) amplification of sequences...
| Autor principal: | Fernandes, Octávio |
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| Outros Autores: | Souto, Ricardo Peres, Castro, José A., Pereira, José Borges, Fernandes, Neide Carrara, Junqueira, Ângela Cristina Veríssimo, Naiff, Roberto Daibes, Barrett, Toby Vincent, Degrave, Wim Maurits S., Zingales, Bianca S., Campbell, David A., Coura, José Rodrigues |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
American Journal of Tropical Medicine and Hygiene
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16452 |
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oai:repositorio:1-16452 |
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oai:repositorio:1-16452 Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences Fernandes, Octávio Souto, Ricardo Peres Castro, José A. Pereira, José Borges Fernandes, Neide Carrara Junqueira, Ângela Cristina Veríssimo Naiff, Roberto Daibes Barrett, Toby Vincent Degrave, Wim Maurits S. Zingales, Bianca S. Campbell, David A. Coura, José Rodrigues Brasil Cistron Disease Transmission Dna Fingerprinting Dna Sequence Human Human Cell Karyotype Parasite Isolation Polymerase Chain Reaction Random Amplified Polymorphic Dna Rna Sequence Trypanosomiasis Traditional molecular and biochemical methods, such as schizodeme analysis, karyotyping, DNA fingerprinting, and enzyme electrophoretic profiles, have shown a large variability among Trypanosoma cruzi isolates. In contrast to those results, polymerase chain reaction (PCR) amplification of sequences from the 24Sα ribosomal RNA gene and from the mini-exon gene nontranscribed spacer indicated a dimorphism among T. cruzi isolates, which enabled the definition of two major parasite lineages. In the present study, 86 T. cruzi field stocks (68 isolated from humans with defined presentations of Chagas' disease and 18 from triatomines) derived from four Brazilian geographic areas were typed by the PCR assay based on the DNA sequences of the mini-exon and 24Sα rRNA genes. These stocks were ordered into the two major T. cruzi lineages. Lineage I was associated mainly with human isolates and lineage 2 with the sylvatic cycle of the parasite. 2020-06-05T18:34:34Z 2020-06-05T18:34:34Z 1998 Artigo https://repositorio.inpa.gov.br/handle/1/16452 10.4269/ajtmh.1998.58.807 en Volume 58, Número 6, Pags. 807-811 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ application/pdf American Journal of Tropical Medicine and Hygiene |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Brasil Cistron Disease Transmission Dna Fingerprinting Dna Sequence Human Human Cell Karyotype Parasite Isolation Polymerase Chain Reaction Random Amplified Polymorphic Dna Rna Sequence Trypanosomiasis |
| spellingShingle |
Brasil Cistron Disease Transmission Dna Fingerprinting Dna Sequence Human Human Cell Karyotype Parasite Isolation Polymerase Chain Reaction Random Amplified Polymorphic Dna Rna Sequence Trypanosomiasis Fernandes, Octávio Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| topic_facet |
Brasil Cistron Disease Transmission Dna Fingerprinting Dna Sequence Human Human Cell Karyotype Parasite Isolation Polymerase Chain Reaction Random Amplified Polymorphic Dna Rna Sequence Trypanosomiasis |
| description |
Traditional molecular and biochemical methods, such as schizodeme analysis, karyotyping, DNA fingerprinting, and enzyme electrophoretic profiles, have shown a large variability among Trypanosoma cruzi isolates. In contrast to those results, polymerase chain reaction (PCR) amplification of sequences from the 24Sα ribosomal RNA gene and from the mini-exon gene nontranscribed spacer indicated a dimorphism among T. cruzi isolates, which enabled the definition of two major parasite lineages. In the present study, 86 T. cruzi field stocks (68 isolated from humans with defined presentations of Chagas' disease and 18 from triatomines) derived from four Brazilian geographic areas were typed by the PCR assay based on the DNA sequences of the mini-exon and 24Sα rRNA genes. These stocks were ordered into the two major T. cruzi lineages. Lineage I was associated mainly with human isolates and lineage 2 with the sylvatic cycle of the parasite. |
| format |
Artigo |
| author |
Fernandes, Octávio |
| author2 |
Souto, Ricardo Peres Castro, José A. Pereira, José Borges Fernandes, Neide Carrara Junqueira, Ângela Cristina Veríssimo Naiff, Roberto Daibes Barrett, Toby Vincent Degrave, Wim Maurits S. Zingales, Bianca S. Campbell, David A. Coura, José Rodrigues |
| author2Str |
Souto, Ricardo Peres Castro, José A. Pereira, José Borges Fernandes, Neide Carrara Junqueira, Ângela Cristina Veríssimo Naiff, Roberto Daibes Barrett, Toby Vincent Degrave, Wim Maurits S. Zingales, Bianca S. Campbell, David A. Coura, José Rodrigues |
| title |
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| title_short |
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| title_full |
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| title_fullStr |
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| title_full_unstemmed |
Brazilian isolates of Trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal RNA sequences |
| title_sort |
brazilian isolates of trypanosoma cruzi from humans and triatomines classified into two lineages using mini-exon and ribosomal rna sequences |
| publisher |
American Journal of Tropical Medicine and Hygiene |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16452 |
| _version_ |
1787142096323346432 |
| score |
11.755432 |