Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females
Karyotypes and chromosomal characteristics with focus on B chromosomes of 2 species of the serrasalmid genus Metynnis, namely M. lippincottianus and M. maculatus, were examined using conventional (C-banding) and molecular (FISH mapping of minor and major rDNAs and Rex1, Rex3, and Rex6 retrotransposa...
| Autor principal: | Favarato, Ramon Marin |
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| Outros Autores: | Braga Ribeiro, Leila, Ota, Rafaela Priscila, Nakayama, Celeste Mutuko, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Cytogenetic and Genome Research
2020
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https://repositorio.inpa.gov.br/handle/1/16645 |
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oai:repositorio:1-16645 |
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oai:repositorio:1-16645 Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females Favarato, Ramon Marin Braga Ribeiro, Leila Ota, Rafaela Priscila Nakayama, Celeste Mutuko Feldberg, Eliana Dna 18s Dna 5s Ribosome Dna Autosome C Banding Characiformes Chromosomal Mapping Cytogenetics Diploidy Female Heterochromatin Karyotype Male Metynnis Lippincottianus Metynnis Maculatus Nonhuman Priority Journal Retroposon Animals Characiformes Chromosome Chromosome Banding Pattern In Situ Hybridization, Fluorescence Genetics Sexual Characteristics Species Difference Ultrastructure Animal Characiformes Chromosome Banding Chromosomes Ribosomal Dna Female Heterochromatin In Situ Hybridization, Fluorescence Karyotype Male Retroelements Sex Characteristics Species Specificity Karyotypes and chromosomal characteristics with focus on B chromosomes of 2 species of the serrasalmid genus Metynnis, namely M. lippincottianus and M. maculatus, were examined using conventional (C-banding) and molecular (FISH mapping of minor and major rDNAs and Rex1, Rex3, and Rex6 retrotransposable elements) protocols. Both species possessed a diploid chromosome number of 2n = 62 and karyotypes composed of 32 metacentric + 28 submetacentric + 2 subtelocentric and 32 metacentric + 26 submetacentric + 4 subtelocentric, respectively; one small B element was found in the female genome of M. lippincottianus. C-banding revealed heterochromatin in the pericentromeric and terminal portions of all chromosomes of both species; the B chromosome was entirely heterochromatic. FISH showed 18S rDNA sites in 2 chromosome pairs in both species (pairs 19 and 22), and a large block in the B chromosome, while 5S rDNA signals were detected in the first pair of subtelocentric chromosomes in both species, moreover in M. maculatus an additional labeled pair 4 was observed. Mapping of the Rex1, Rex3, and Rex6 retrotransposable elements in the genomes of M. lippincottianus and M. maculatus indicated that they were dispersed throughout nearly all the chromosomes of the complement, except for the B chromosome of M. lippincottianus. © 2019 S. Karger AG, Basel. 2020-06-15T21:35:34Z 2020-06-15T21:35:34Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/16645 10.1159/000499954 en Volume 158, Número 1, Pags. 38-45 Restrito Cytogenetic and Genome Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna 18s Dna 5s Ribosome Dna Autosome C Banding Characiformes Chromosomal Mapping Cytogenetics Diploidy Female Heterochromatin Karyotype Male Metynnis Lippincottianus Metynnis Maculatus Nonhuman Priority Journal Retroposon Animals Characiformes Chromosome Chromosome Banding Pattern In Situ Hybridization, Fluorescence Genetics Sexual Characteristics Species Difference Ultrastructure Animal Characiformes Chromosome Banding Chromosomes Ribosomal Dna Female Heterochromatin In Situ Hybridization, Fluorescence Karyotype Male Retroelements Sex Characteristics Species Specificity |
| spellingShingle |
Dna 18s Dna 5s Ribosome Dna Autosome C Banding Characiformes Chromosomal Mapping Cytogenetics Diploidy Female Heterochromatin Karyotype Male Metynnis Lippincottianus Metynnis Maculatus Nonhuman Priority Journal Retroposon Animals Characiformes Chromosome Chromosome Banding Pattern In Situ Hybridization, Fluorescence Genetics Sexual Characteristics Species Difference Ultrastructure Animal Characiformes Chromosome Banding Chromosomes Ribosomal Dna Female Heterochromatin In Situ Hybridization, Fluorescence Karyotype Male Retroelements Sex Characteristics Species Specificity Favarato, Ramon Marin Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| topic_facet |
Dna 18s Dna 5s Ribosome Dna Autosome C Banding Characiformes Chromosomal Mapping Cytogenetics Diploidy Female Heterochromatin Karyotype Male Metynnis Lippincottianus Metynnis Maculatus Nonhuman Priority Journal Retroposon Animals Characiformes Chromosome Chromosome Banding Pattern In Situ Hybridization, Fluorescence Genetics Sexual Characteristics Species Difference Ultrastructure Animal Characiformes Chromosome Banding Chromosomes Ribosomal Dna Female Heterochromatin In Situ Hybridization, Fluorescence Karyotype Male Retroelements Sex Characteristics Species Specificity |
| description |
Karyotypes and chromosomal characteristics with focus on B chromosomes of 2 species of the serrasalmid genus Metynnis, namely M. lippincottianus and M. maculatus, were examined using conventional (C-banding) and molecular (FISH mapping of minor and major rDNAs and Rex1, Rex3, and Rex6 retrotransposable elements) protocols. Both species possessed a diploid chromosome number of 2n = 62 and karyotypes composed of 32 metacentric + 28 submetacentric + 2 subtelocentric and 32 metacentric + 26 submetacentric + 4 subtelocentric, respectively; one small B element was found in the female genome of M. lippincottianus. C-banding revealed heterochromatin in the pericentromeric and terminal portions of all chromosomes of both species; the B chromosome was entirely heterochromatic. FISH showed 18S rDNA sites in 2 chromosome pairs in both species (pairs 19 and 22), and a large block in the B chromosome, while 5S rDNA signals were detected in the first pair of subtelocentric chromosomes in both species, moreover in M. maculatus an additional labeled pair 4 was observed. Mapping of the Rex1, Rex3, and Rex6 retrotransposable elements in the genomes of M. lippincottianus and M. maculatus indicated that they were dispersed throughout nearly all the chromosomes of the complement, except for the B chromosome of M. lippincottianus. © 2019 S. Karger AG, Basel. |
| format |
Artigo |
| author |
Favarato, Ramon Marin |
| author2 |
Braga Ribeiro, Leila Ota, Rafaela Priscila Nakayama, Celeste Mutuko Feldberg, Eliana |
| author2Str |
Braga Ribeiro, Leila Ota, Rafaela Priscila Nakayama, Celeste Mutuko Feldberg, Eliana |
| title |
Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| title_short |
Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| title_full |
Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| title_fullStr |
Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| title_full_unstemmed |
Cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| title_sort |
cytogenetic characterization of two metynnis species (characiformes, serrasalmidae) reveals b chromosomes restricted to the females |
| publisher |
Cytogenetic and Genome Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16645 |
| _version_ |
1787141769690873856 |
| score |
11.755432 |