Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides)
Undifferentiated spermatogonia (A und ) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several s...
| Autor principal: | Costa, Guilherme M.J. |
|---|---|
| Outros Autores: | Sousa, Alana Lislea, Figueiredo, Andre´ Felipe Almeida, Lacerda, Samyra Maria Santos N.Nassif, França, Luiz Renato de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
General and Comparative Endocrinology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16725 |
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oai:repositorio:1-16725 |
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oai:repositorio:1-16725 Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) Costa, Guilherme M.J. Sousa, Alana Lislea Figueiredo, Andre´ Felipe Almeida Lacerda, Samyra Maria Santos N.Nassif França, Luiz Renato de Colony Stimulating Factor 1 Receptor Colony Stimulating Factor Receptor Gdnf Family Receptor Alpha 1 Glial Cell Line Derived Neurotrophic Factor Receptor Unclassified Drug Biological Marker Glial Cell Line Derived Neurotrophic Factor Receptor Granulocyte Macrophage Colony Stimulating Factor Receptor Adult Animals Cell Animals Experiment Animals Tissue Cell Culture Cell Size Cell Structure Cellular Distribution Controlled Study Cryopreservation Evolution Genetic Conservation Kinosternon Scorpioides Male Nonhuman Priority Journal Protein Expression Spermatogonium Stem Cell Niche Turtle Animals Cell Shape Cytology Metabolism Scorpion South America Spermatogonium Stem Cell Turtle Animal Biomarkers Cell Shape Cell Size Glial Cell Line-derived Neurotrophic Factor Receptors Male Receptors, Granulocyte-macrophage Colony-stimulating Factor Scorpions South America Spermatogonia Stem Cell Niche Stem Cells Turtles Undifferentiated spermatogonia (A und ) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been developed on SSCs in mammalian testis, little is known about other vertebrate classes. The present study is the first to perform a more detailed investigation on the spermatogonial cells and their niche in a reptilian species. Thus, we characterized A und /SSCs and evaluated the existence of SSCs niche in the Kinosternon scorpioides, a freshwater turtle found from Mexico to northern and central South America. Our results showed that, in this species, A und /SSCs exhibited a nuclear morphological pattern similar to those described for other mammalian species already investigated. However, in comparison to other spermatogonial cell types, A und /SSCs presented the largest nuclear volume in this turtle. Similar to some mammalian and fish species investigated, both GFRA1 and CSF1 receptors were expressed in A und /SSCs in K. scorpioides. Also, as K. scorpioides A und /SSCs were preferentially located near blood vessels, it can be suggested that this niche characteristic is a well conserved feature during evolution. Besides being valuable for comparative reproductive biology, our findings represent an important step towards the understanding of SSCs biology and the development of valuable systems/tools for SSCs culture and cryopreservation in turtles. Moreover, we expect that the above-mentioned results will be useful for reproductive biotechnologies as well as for governmental programs aiming at reptilian species conservation. © 2018 Elsevier Inc. 2020-06-15T21:35:58Z 2020-06-15T21:35:58Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/16725 10.1016/j.ygcen.2018.06.019 en Volume 273, Pags. 163-171 Restrito General and Comparative Endocrinology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Colony Stimulating Factor 1 Receptor Colony Stimulating Factor Receptor Gdnf Family Receptor Alpha 1 Glial Cell Line Derived Neurotrophic Factor Receptor Unclassified Drug Biological Marker Glial Cell Line Derived Neurotrophic Factor Receptor Granulocyte Macrophage Colony Stimulating Factor Receptor Adult Animals Cell Animals Experiment Animals Tissue Cell Culture Cell Size Cell Structure Cellular Distribution Controlled Study Cryopreservation Evolution Genetic Conservation Kinosternon Scorpioides Male Nonhuman Priority Journal Protein Expression Spermatogonium Stem Cell Niche Turtle Animals Cell Shape Cytology Metabolism Scorpion South America Spermatogonium Stem Cell Turtle Animal Biomarkers Cell Shape Cell Size Glial Cell Line-derived Neurotrophic Factor Receptors Male Receptors, Granulocyte-macrophage Colony-stimulating Factor Scorpions South America Spermatogonia Stem Cell Niche Stem Cells Turtles |
| spellingShingle |
Colony Stimulating Factor 1 Receptor Colony Stimulating Factor Receptor Gdnf Family Receptor Alpha 1 Glial Cell Line Derived Neurotrophic Factor Receptor Unclassified Drug Biological Marker Glial Cell Line Derived Neurotrophic Factor Receptor Granulocyte Macrophage Colony Stimulating Factor Receptor Adult Animals Cell Animals Experiment Animals Tissue Cell Culture Cell Size Cell Structure Cellular Distribution Controlled Study Cryopreservation Evolution Genetic Conservation Kinosternon Scorpioides Male Nonhuman Priority Journal Protein Expression Spermatogonium Stem Cell Niche Turtle Animals Cell Shape Cytology Metabolism Scorpion South America Spermatogonium Stem Cell Turtle Animal Biomarkers Cell Shape Cell Size Glial Cell Line-derived Neurotrophic Factor Receptors Male Receptors, Granulocyte-macrophage Colony-stimulating Factor Scorpions South America Spermatogonia Stem Cell Niche Stem Cells Turtles Costa, Guilherme M.J. Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| topic_facet |
Colony Stimulating Factor 1 Receptor Colony Stimulating Factor Receptor Gdnf Family Receptor Alpha 1 Glial Cell Line Derived Neurotrophic Factor Receptor Unclassified Drug Biological Marker Glial Cell Line Derived Neurotrophic Factor Receptor Granulocyte Macrophage Colony Stimulating Factor Receptor Adult Animals Cell Animals Experiment Animals Tissue Cell Culture Cell Size Cell Structure Cellular Distribution Controlled Study Cryopreservation Evolution Genetic Conservation Kinosternon Scorpioides Male Nonhuman Priority Journal Protein Expression Spermatogonium Stem Cell Niche Turtle Animals Cell Shape Cytology Metabolism Scorpion South America Spermatogonium Stem Cell Turtle Animal Biomarkers Cell Shape Cell Size Glial Cell Line-derived Neurotrophic Factor Receptors Male Receptors, Granulocyte-macrophage Colony-stimulating Factor Scorpions South America Spermatogonia Stem Cell Niche Stem Cells Turtles |
| description |
Undifferentiated spermatogonia (A und ) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been developed on SSCs in mammalian testis, little is known about other vertebrate classes. The present study is the first to perform a more detailed investigation on the spermatogonial cells and their niche in a reptilian species. Thus, we characterized A und /SSCs and evaluated the existence of SSCs niche in the Kinosternon scorpioides, a freshwater turtle found from Mexico to northern and central South America. Our results showed that, in this species, A und /SSCs exhibited a nuclear morphological pattern similar to those described for other mammalian species already investigated. However, in comparison to other spermatogonial cell types, A und /SSCs presented the largest nuclear volume in this turtle. Similar to some mammalian and fish species investigated, both GFRA1 and CSF1 receptors were expressed in A und /SSCs in K. scorpioides. Also, as K. scorpioides A und /SSCs were preferentially located near blood vessels, it can be suggested that this niche characteristic is a well conserved feature during evolution. Besides being valuable for comparative reproductive biology, our findings represent an important step towards the understanding of SSCs biology and the development of valuable systems/tools for SSCs culture and cryopreservation in turtles. Moreover, we expect that the above-mentioned results will be useful for reproductive biotechnologies as well as for governmental programs aiming at reptilian species conservation. © 2018 Elsevier Inc. |
| format |
Artigo |
| author |
Costa, Guilherme M.J. |
| author2 |
Sousa, Alana Lislea Figueiredo, Andre´ Felipe Almeida Lacerda, Samyra Maria Santos N.Nassif França, Luiz Renato de |
| author2Str |
Sousa, Alana Lislea Figueiredo, Andre´ Felipe Almeida Lacerda, Samyra Maria Santos N.Nassif França, Luiz Renato de |
| title |
Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| title_short |
Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| title_full |
Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| title_fullStr |
Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| title_full_unstemmed |
Characterization of spermatogonial cells and niche in the scorpion mud turtle (Kinosternon scorpioides) |
| title_sort |
characterization of spermatogonial cells and niche in the scorpion mud turtle (kinosternon scorpioides) |
| publisher |
General and Comparative Endocrinology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16725 |
| _version_ |
1787144335944318976 |
| score |
11.653393 |