A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries
Our study assessed the efficiency of a formulated new extender in maintaining viability and morphological integrity of Colossoma macropomum spermatozoa under chilling storage. Semen was diluted in the test extender and BTS™ (Beltsville Thawing Solution) and exposed to a short-term storage at 4.6 ± 0...
| Autor principal: | Pastrana, Yugo Moraes |
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| Outros Autores: | Streit, Danilo Pedro, Garcia, Raycon Roberto Freitas, Becker, Bruno Silveira, Rodrigues, Joséluiz Luiz R., Godoy, Leandro Cesar |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Aquaculture Research
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16745 |
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oai:repositorio:1-16745 |
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oai:repositorio:1-16745 A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries Pastrana, Yugo Moraes Streit, Danilo Pedro Garcia, Raycon Roberto Freitas Becker, Bruno Silveira Rodrigues, Joséluiz Luiz R. Godoy, Leandro Cesar Cell Experiment Finfish Glucose Injury Low Temperature Morphology Sperm Storage Viability Colossoma Macropomum Colossoma Marcopomum Our study assessed the efficiency of a formulated new extender in maintaining viability and morphological integrity of Colossoma macropomum spermatozoa under chilling storage. Semen was diluted in the test extender and BTS™ (Beltsville Thawing Solution) and exposed to a short-term storage at 4.6 ± 0.6°C for 96 hr. Both extenders were able to maintain 17% ± 8% motile spermatozoa by the end of experiment. Sperm dilution in test extender did not affect the morphologically normal cells (61% ± 6%) up to 48 hr of chilling, being higher than in BTS™ (50% ± 6%) (p < 0.05). After 96 hr, samples kept in the test extender had 50% of normal spermatozoa, whereas those kept in BTS™ presented only 38% of normal cells. Chilling storage increased the incidence of cells with strongly coiled flagella in BTS ™ . Our study is the first to evaluate in detail the spermatozoa morphology as indicative of C. macropomum semen viability. The new extender was able to protect the spermatozoa against increase in coiled flagellum injuries. © 2018 John Wiley & Sons Ltd 2020-06-15T21:36:05Z 2020-06-15T21:36:05Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/16745 10.1111/are.13923 en Volume 50, Número 2, Pags. 521-528 Restrito Aquaculture Research |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Cell Experiment Finfish Glucose Injury Low Temperature Morphology Sperm Storage Viability Colossoma Macropomum Colossoma Marcopomum |
| spellingShingle |
Cell Experiment Finfish Glucose Injury Low Temperature Morphology Sperm Storage Viability Colossoma Macropomum Colossoma Marcopomum Pastrana, Yugo Moraes A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| topic_facet |
Cell Experiment Finfish Glucose Injury Low Temperature Morphology Sperm Storage Viability Colossoma Macropomum Colossoma Marcopomum |
| description |
Our study assessed the efficiency of a formulated new extender in maintaining viability and morphological integrity of Colossoma macropomum spermatozoa under chilling storage. Semen was diluted in the test extender and BTS™ (Beltsville Thawing Solution) and exposed to a short-term storage at 4.6 ± 0.6°C for 96 hr. Both extenders were able to maintain 17% ± 8% motile spermatozoa by the end of experiment. Sperm dilution in test extender did not affect the morphologically normal cells (61% ± 6%) up to 48 hr of chilling, being higher than in BTS™ (50% ± 6%) (p < 0.05). After 96 hr, samples kept in the test extender had 50% of normal spermatozoa, whereas those kept in BTS™ presented only 38% of normal cells. Chilling storage increased the incidence of cells with strongly coiled flagella in BTS ™ . Our study is the first to evaluate in detail the spermatozoa morphology as indicative of C. macropomum semen viability. The new extender was able to protect the spermatozoa against increase in coiled flagellum injuries. © 2018 John Wiley & Sons Ltd |
| format |
Artigo |
| author |
Pastrana, Yugo Moraes |
| author2 |
Streit, Danilo Pedro Garcia, Raycon Roberto Freitas Becker, Bruno Silveira Rodrigues, Joséluiz Luiz R. Godoy, Leandro Cesar |
| author2Str |
Streit, Danilo Pedro Garcia, Raycon Roberto Freitas Becker, Bruno Silveira Rodrigues, Joséluiz Luiz R. Godoy, Leandro Cesar |
| title |
A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| title_short |
A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| title_full |
A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| title_fullStr |
A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| title_full_unstemmed |
A fructose-based extender protects Colossoma macropomum spermatozoa against chilling injuries |
| title_sort |
fructose-based extender protects colossoma macropomum spermatozoa against chilling injuries |
| publisher |
Aquaculture Research |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16745 |
| _version_ |
1787144125316857856 |
| score |
11.674684 |