The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil

Costa, Oscar Tadeu Ferreira da; Dias, Lucas Castanhola; Malmann, Carlos Augusto; Lima Ferreira, César Augusto de; Carmo, Iracimar Batista Do; Wischneski, Andrew Georg; Sousa, Rafael Luckwu de; Cavero, Bruno Adan Sagratzki; Lameiras, Juliana Luiza Varjão; Santos, Maria Cristina dos

Artigo

The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil

Farming of Amazonian native fish species holds great promise. One species whose characteristics make it suitable for intensive farming is tambaqui (Colossoma macropomum). However, there is a lack of consensus about the most suitable stocking density (SD) for intensive farming. More information on th...

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Autor principal:	Costa, Oscar Tadeu Ferreira da
Outros Autores:	Dias, Lucas Castanhola, Malmann, Carlos Augusto, Lima Ferreira, César Augusto de, Carmo, Iracimar Batista Do, Wischneski, Andrew Georg, Sousa, Rafael Luckwu de, Cavero, Bruno Adan Sagratzki, Lameiras, Juliana Luiza Varjão, Santos, Maria Cristina dos
Grau:	Artigo
Idioma:	English
Publicado em:	Aquaculture 2020
Assuntos:	Electrokinesis Farming System Hematology Immune Response Intensive Culture Juvenile Native Species Parasite Physiology Plasma Protein Stocking Density Teleost Water Quality Brasil Colossoma Macropomum Colossoma Marcopomum
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/16753

id	oai:repositorio:1-16753
recordtype	dspace
spelling	oai:repositorio:1-16753 The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil Costa, Oscar Tadeu Ferreira da Dias, Lucas Castanhola Malmann, Carlos Augusto Lima Ferreira, César Augusto de Carmo, Iracimar Batista Do Wischneski, Andrew Georg Sousa, Rafael Luckwu de Cavero, Bruno Adan Sagratzki Lameiras, Juliana Luiza Varjão Santos, Maria Cristina dos Electrokinesis Farming System Hematology Immune Response Intensive Culture Juvenile Native Species Parasite Physiology Plasma Protein Stocking Density Teleost Water Quality Brasil Colossoma Macropomum Colossoma Marcopomum Farming of Amazonian native fish species holds great promise. One species whose characteristics make it suitable for intensive farming is tambaqui (Colossoma macropomum). However, there is a lack of consensus about the most suitable stocking density (SD) for intensive farming. More information on the physiology of fish in farming systems is thus required, as this would increase our understanding of the effects of stress on these fish. The aim of this study was therefore to evaluate the effect of different SDs on the hematology and plasma protein profile of tambaqui. To this end, juvenile tambaqui were exposed to the following different SDs (kg/m3) for 96 h: SD1 = 2.11; SD2 = 8.31; SD3 = 16.34; and SD4 = 27.40. Water quality was monitored, and blood was collected for hematological analysis and electrophoresis (SDS-PAGE) to analyze plasma protein and immunoglobulin profiles. Gill filaments were removed and the number of parasites (monogenoids) was counted. Multivariate PCA analysis revealed a strong positive association between the highest SDs (SD3 and SD4) and parasite count, immune cells (eosinophils, thrombocytes, monocytes and lymphocytes) and water ammonia and nitrite levels. Conversely, the highest SDs were negatively associated with water quality variables (pH, turbidity, conductivity and O2 content) and blood hematocrit. The results show that the highest SDs contributed to a deterioration in water quality, leading to increased MetHb levels, gill parasitism, an increase in the number of defense cells, altered protein profile and stimulation of immunoglobulin production. These changes suggest that the welfare of tambaqui is adversely affected by high SDs and increase our understanding of the effects of crowding stress on the physiology of the species. © 2018 2020-06-15T21:36:08Z 2020-06-15T21:36:08Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/16753 10.1016/j.aquaculture.2018.09.040 en Volume 499, Pags. 260-268 Restrito Aquaculture
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Electrokinesis Farming System Hematology Immune Response Intensive Culture Juvenile Native Species Parasite Physiology Plasma Protein Stocking Density Teleost Water Quality Brasil Colossoma Macropomum Colossoma Marcopomum
spellingShingle	Electrokinesis Farming System Hematology Immune Response Intensive Culture Juvenile Native Species Parasite Physiology Plasma Protein Stocking Density Teleost Water Quality Brasil Colossoma Macropomum Colossoma Marcopomum Costa, Oscar Tadeu Ferreira da The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
topic_facet	Electrokinesis Farming System Hematology Immune Response Intensive Culture Juvenile Native Species Parasite Physiology Plasma Protein Stocking Density Teleost Water Quality Brasil Colossoma Macropomum Colossoma Marcopomum
description	Farming of Amazonian native fish species holds great promise. One species whose characteristics make it suitable for intensive farming is tambaqui (Colossoma macropomum). However, there is a lack of consensus about the most suitable stocking density (SD) for intensive farming. More information on the physiology of fish in farming systems is thus required, as this would increase our understanding of the effects of stress on these fish. The aim of this study was therefore to evaluate the effect of different SDs on the hematology and plasma protein profile of tambaqui. To this end, juvenile tambaqui were exposed to the following different SDs (kg/m3) for 96 h: SD1 = 2.11; SD2 = 8.31; SD3 = 16.34; and SD4 = 27.40. Water quality was monitored, and blood was collected for hematological analysis and electrophoresis (SDS-PAGE) to analyze plasma protein and immunoglobulin profiles. Gill filaments were removed and the number of parasites (monogenoids) was counted. Multivariate PCA analysis revealed a strong positive association between the highest SDs (SD3 and SD4) and parasite count, immune cells (eosinophils, thrombocytes, monocytes and lymphocytes) and water ammonia and nitrite levels. Conversely, the highest SDs were negatively associated with water quality variables (pH, turbidity, conductivity and O2 content) and blood hematocrit. The results show that the highest SDs contributed to a deterioration in water quality, leading to increased MetHb levels, gill parasitism, an increase in the number of defense cells, altered protein profile and stimulation of immunoglobulin production. These changes suggest that the welfare of tambaqui is adversely affected by high SDs and increase our understanding of the effects of crowding stress on the physiology of the species. © 2018
format	Artigo
author	Costa, Oscar Tadeu Ferreira da
author2	Dias, Lucas Castanhola Malmann, Carlos Augusto Lima Ferreira, César Augusto de Carmo, Iracimar Batista Do Wischneski, Andrew Georg Sousa, Rafael Luckwu de Cavero, Bruno Adan Sagratzki Lameiras, Juliana Luiza Varjão Santos, Maria Cristina dos
author2Str	Dias, Lucas Castanhola Malmann, Carlos Augusto Lima Ferreira, César Augusto de Carmo, Iracimar Batista Do Wischneski, Andrew Georg Sousa, Rafael Luckwu de Cavero, Bruno Adan Sagratzki Lameiras, Juliana Luiza Varjão Santos, Maria Cristina dos
title	The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
title_short	The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
title_full	The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
title_fullStr	The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
title_full_unstemmed	The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil
title_sort	effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (colossoma macropomum) farmed in brazil
publisher	Aquaculture
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/16753
_version_	1787141480822865920
score	11.755432

The effects of stocking density on the hematology, plasma protein profile and immunoglobulin production of juvenile tambaqui (Colossoma macropomum) farmed in Brazil

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