Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis
The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure su...
| Autor principal: | Heinrichs-Caldas, Waldir D. |
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| Outros Autores: | Campos, Derek Felipe de, Paula-Silva, Maria Nazaré N., Almeida-Val, Vera Maria Fonseca |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology
2020
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https://repositorio.inpa.gov.br/handle/1/16764 |
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oai:repositorio:1-16764 |
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oai:repositorio:1-16764 Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis Heinrichs-Caldas, Waldir D. Campos, Derek Felipe de Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca Citrate Synthase Glucose Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Lactic Acid Malate Dehydrogenase Oxidoreductase Oxygen Fish Protein Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Aerobic Metabolism Anaerobic Capacity Anaerobic Metabolism Animals Tissue Astronotus Crassipinnis Controlled Study Enzyme Activity Female Gene Expression Glucose Blood Level Glycolysis Hypoxia Lactate Blood Level Liver Male Nonhuman Oxygen Concentration Oxygen Consumption Perciformes Priority Journal Respirometry Muscle, Skeletal Adaptation Animals Antibody Specificity Aquaculture Biological Model Cell Hypoxia Cichlid Classification Energy Metabolism Enzymology Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Physiology Randomization Time Factor Adaptation, Physiological Animal Aquaculture Cell Hypoxia Cichlids Energy Metabolism Female Fish Proteins Gene Expression Regulation, Developmental Glycolysis Hypoxia-inducible Factor 1, Alpha Subunit Lactate Dehydrogenases Liver Male Models, Biological Muscle, Skeletal Organ Specificity Oxygen Consumption Random Allocation Time Factors The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure such naturally variable environments. Hypoxia-Inducible factor-1α (hif-1α) is among the most important and studied genes related to hypoxia-tolerance, maintaining regular cellular function and controlling anaerobic metabolism. In the present work, we studied hif-1α expression and related it to changes in metabolic pathways of Astronotus crassipinnis exposed to 1, 3 and 5 h of hypoxia, followed by 3 h of recovery. The results show that A. crassipinnis depresses aerobic metabolic under hypoxia, with a decrease in glycolysis and oxidative enzyme activities, and increases its anaerobic metabolism with an increase in LDH activity coupled with a decrease in oxygen consumption, which indicates an increase in anaerobic capacity. In addition, the animal differentially regulates hif-1α gene in each tissue studied, with a positive relationship to its metabolic profile, suggesting that hif-1α might be one of the most important induction factors that regulate hypoxia tolerance in this species. © 2018 Elsevier Inc. 2020-06-15T21:36:11Z 2020-06-15T21:36:11Z 2019 Artigo https://repositorio.inpa.gov.br/handle/1/16764 10.1016/j.cbpb.2018.08.011 en Volume 227, Pags. 31-38 Restrito Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Citrate Synthase Glucose Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Lactic Acid Malate Dehydrogenase Oxidoreductase Oxygen Fish Protein Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Aerobic Metabolism Anaerobic Capacity Anaerobic Metabolism Animals Tissue Astronotus Crassipinnis Controlled Study Enzyme Activity Female Gene Expression Glucose Blood Level Glycolysis Hypoxia Lactate Blood Level Liver Male Nonhuman Oxygen Concentration Oxygen Consumption Perciformes Priority Journal Respirometry Muscle, Skeletal Adaptation Animals Antibody Specificity Aquaculture Biological Model Cell Hypoxia Cichlid Classification Energy Metabolism Enzymology Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Physiology Randomization Time Factor Adaptation, Physiological Animal Aquaculture Cell Hypoxia Cichlids Energy Metabolism Female Fish Proteins Gene Expression Regulation, Developmental Glycolysis Hypoxia-inducible Factor 1, Alpha Subunit Lactate Dehydrogenases Liver Male Models, Biological Muscle, Skeletal Organ Specificity Oxygen Consumption Random Allocation Time Factors |
| spellingShingle |
Citrate Synthase Glucose Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Lactic Acid Malate Dehydrogenase Oxidoreductase Oxygen Fish Protein Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Aerobic Metabolism Anaerobic Capacity Anaerobic Metabolism Animals Tissue Astronotus Crassipinnis Controlled Study Enzyme Activity Female Gene Expression Glucose Blood Level Glycolysis Hypoxia Lactate Blood Level Liver Male Nonhuman Oxygen Concentration Oxygen Consumption Perciformes Priority Journal Respirometry Muscle, Skeletal Adaptation Animals Antibody Specificity Aquaculture Biological Model Cell Hypoxia Cichlid Classification Energy Metabolism Enzymology Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Physiology Randomization Time Factor Adaptation, Physiological Animal Aquaculture Cell Hypoxia Cichlids Energy Metabolism Female Fish Proteins Gene Expression Regulation, Developmental Glycolysis Hypoxia-inducible Factor 1, Alpha Subunit Lactate Dehydrogenases Liver Male Models, Biological Muscle, Skeletal Organ Specificity Oxygen Consumption Random Allocation Time Factors Heinrichs-Caldas, Waldir D. Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| topic_facet |
Citrate Synthase Glucose Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Lactic Acid Malate Dehydrogenase Oxidoreductase Oxygen Fish Protein Hypoxia Inducible Factor 1alpha Lactate Dehydrogenase Aerobic Metabolism Anaerobic Capacity Anaerobic Metabolism Animals Tissue Astronotus Crassipinnis Controlled Study Enzyme Activity Female Gene Expression Glucose Blood Level Glycolysis Hypoxia Lactate Blood Level Liver Male Nonhuman Oxygen Concentration Oxygen Consumption Perciformes Priority Journal Respirometry Muscle, Skeletal Adaptation Animals Antibody Specificity Aquaculture Biological Model Cell Hypoxia Cichlid Classification Energy Metabolism Enzymology Gene Expression Regulation Genetics Growth, Development And Aging Metabolism Physiology Randomization Time Factor Adaptation, Physiological Animal Aquaculture Cell Hypoxia Cichlids Energy Metabolism Female Fish Proteins Gene Expression Regulation, Developmental Glycolysis Hypoxia-inducible Factor 1, Alpha Subunit Lactate Dehydrogenases Liver Male Models, Biological Muscle, Skeletal Organ Specificity Oxygen Consumption Random Allocation Time Factors |
| description |
The aquatic habitats of the Amazon basin present dramatic variation of oxygen level, and, to survive such changes, many aquatic animals developed biochemical and physiological adaptations. The advanced teleost Astronotus crassipinnis (Perciformes) is a fish tolerant to hypoxia and known to endure such naturally variable environments. Hypoxia-Inducible factor-1α (hif-1α) is among the most important and studied genes related to hypoxia-tolerance, maintaining regular cellular function and controlling anaerobic metabolism. In the present work, we studied hif-1α expression and related it to changes in metabolic pathways of Astronotus crassipinnis exposed to 1, 3 and 5 h of hypoxia, followed by 3 h of recovery. The results show that A. crassipinnis depresses aerobic metabolic under hypoxia, with a decrease in glycolysis and oxidative enzyme activities, and increases its anaerobic metabolism with an increase in LDH activity coupled with a decrease in oxygen consumption, which indicates an increase in anaerobic capacity. In addition, the animal differentially regulates hif-1α gene in each tissue studied, with a positive relationship to its metabolic profile, suggesting that hif-1α might be one of the most important induction factors that regulate hypoxia tolerance in this species. © 2018 Elsevier Inc. |
| format |
Artigo |
| author |
Heinrichs-Caldas, Waldir D. |
| author2 |
Campos, Derek Felipe de Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca |
| author2Str |
Campos, Derek Felipe de Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca |
| title |
Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| title_short |
Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| title_full |
Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| title_fullStr |
Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| title_full_unstemmed |
Oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the Amazon Oscar, Astronotus crassipinnis |
| title_sort |
oxygen-dependent distinct expression of hif-1α gene in aerobic and anaerobic tissues of the amazon oscar, astronotus crassipinnis |
| publisher |
Comparative Biochemistry and Physiology. Part B: Biochemistry & Molecular Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16764 |
| _version_ |
1787144336241065984 |
| score |
11.674752 |