Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™
The aim of this study was to evaluate if hormonal treatment causes changes in the morphology of matrinxã Brycon amazonicus spermatozoa. Twelve males were randomized into three treatment groups: 1, Ovaprim™ (0.5 ml/kg); 2, carp pituitary extract (CPE; 2.0 mg/kg); and 3, NaCl solution 0.9% - control g...
| Autor principal: | Oliveira, Rosilane Gomes Souza de |
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| Outros Autores: | Oliveira, Arley Faria José de, Villacorta-Corrêa, Marle Angélica |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Zygote
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/16881 |
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oai:repositorio:1-16881 |
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oai:repositorio:1-16881 Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ Oliveira, Rosilane Gomes Souza de Oliveira, Arley Faria José de Villacorta-Corrêa, Marle Angélica Domperidone Gonadorelin Hypophysis Hormone Ovaprim Tissue Extract Animals Carp Characiformes Drug Combination Drug Effect Male Pathology Physiology Spermatozoon Spermatozoon Motility Animal Carps Characiformes Domperidone Drug Combinations Gonadotropin-releasing Hormone Male Pituitary Hormones Sperm Motility Spermatozoa Tissue Extracts The aim of this study was to evaluate if hormonal treatment causes changes in the morphology of matrinxã Brycon amazonicus spermatozoa. Twelve males were randomized into three treatment groups: 1, Ovaprim™ (0.5 ml/kg); 2, carp pituitary extract (CPE; 2.0 mg/kg); and 3, NaCl solution 0.9% - control group (0.5 ml/kg); with four replicates. Morphological sperm analysis was performed using an optical and scanning electron microscope. The percentage of normal spermatozoa (49.6±4.6% to 60.8±2.8%), with primary (26.1±6.4% to 45.3±4.5%) and secondary abnormalities (4.4±2.9% to 13.9±3.5%) did not differ significantly between treatment groups. There were no significant differences between treatments in relation to the primary abnormalities found in the head (10.5±3.8% to 25.5±6.3%), the midpiece (0.1±0.1% to 0.2±0.2%) and in the flagellum (18.9±3.8% to 15.5±3.1%), as well as in the secondary abnormalities, located in the head (0.8±0.4% to 7.8±4.6%) and in the flagellum (2.2±1.1% to 6.1±1.5%). When each abnormality was evaluated individually, only the percentage of degenerated head was higher in the CPE-induced group (24.9±5.9%) than the control group (7.2±3.1%). We concluded that the use of pituitary extract of carp or Ovaprim™ under the conditions of this experiment does not influence the percentage of sperm abnormalities in B. amazonicus. © Cambridge University Press 2018. 2020-06-15T21:36:58Z 2020-06-15T21:36:58Z 2018 Artigo https://repositorio.inpa.gov.br/handle/1/16881 10.1017/S0967199418000199 en Volume 26, Número 4, Pags. 279-285 Restrito Zygote |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Domperidone Gonadorelin Hypophysis Hormone Ovaprim Tissue Extract Animals Carp Characiformes Drug Combination Drug Effect Male Pathology Physiology Spermatozoon Spermatozoon Motility Animal Carps Characiformes Domperidone Drug Combinations Gonadotropin-releasing Hormone Male Pituitary Hormones Sperm Motility Spermatozoa Tissue Extracts |
| spellingShingle |
Domperidone Gonadorelin Hypophysis Hormone Ovaprim Tissue Extract Animals Carp Characiformes Drug Combination Drug Effect Male Pathology Physiology Spermatozoon Spermatozoon Motility Animal Carps Characiformes Domperidone Drug Combinations Gonadotropin-releasing Hormone Male Pituitary Hormones Sperm Motility Spermatozoa Tissue Extracts Oliveira, Rosilane Gomes Souza de Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| topic_facet |
Domperidone Gonadorelin Hypophysis Hormone Ovaprim Tissue Extract Animals Carp Characiformes Drug Combination Drug Effect Male Pathology Physiology Spermatozoon Spermatozoon Motility Animal Carps Characiformes Domperidone Drug Combinations Gonadotropin-releasing Hormone Male Pituitary Hormones Sperm Motility Spermatozoa Tissue Extracts |
| description |
The aim of this study was to evaluate if hormonal treatment causes changes in the morphology of matrinxã Brycon amazonicus spermatozoa. Twelve males were randomized into three treatment groups: 1, Ovaprim™ (0.5 ml/kg); 2, carp pituitary extract (CPE; 2.0 mg/kg); and 3, NaCl solution 0.9% - control group (0.5 ml/kg); with four replicates. Morphological sperm analysis was performed using an optical and scanning electron microscope. The percentage of normal spermatozoa (49.6±4.6% to 60.8±2.8%), with primary (26.1±6.4% to 45.3±4.5%) and secondary abnormalities (4.4±2.9% to 13.9±3.5%) did not differ significantly between treatment groups. There were no significant differences between treatments in relation to the primary abnormalities found in the head (10.5±3.8% to 25.5±6.3%), the midpiece (0.1±0.1% to 0.2±0.2%) and in the flagellum (18.9±3.8% to 15.5±3.1%), as well as in the secondary abnormalities, located in the head (0.8±0.4% to 7.8±4.6%) and in the flagellum (2.2±1.1% to 6.1±1.5%). When each abnormality was evaluated individually, only the percentage of degenerated head was higher in the CPE-induced group (24.9±5.9%) than the control group (7.2±3.1%). We concluded that the use of pituitary extract of carp or Ovaprim™ under the conditions of this experiment does not influence the percentage of sperm abnormalities in B. amazonicus. © Cambridge University Press 2018. |
| format |
Artigo |
| author |
Oliveira, Rosilane Gomes Souza de |
| author2 |
Oliveira, Arley Faria José de Villacorta-Corrêa, Marle Angélica |
| author2Str |
Oliveira, Arley Faria José de Villacorta-Corrêa, Marle Angélica |
| title |
Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| title_short |
Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| title_full |
Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| title_fullStr |
Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| title_full_unstemmed |
Sperm abnormalities in matrinxã Brycon amazonicus after hormonal treatment with carp pituitary extract and Ovaprim™ |
| title_sort |
sperm abnormalities in matrinxã brycon amazonicus after hormonal treatment with carp pituitary extract and ovaprim™ |
| publisher |
Zygote |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/16881 |
| _version_ |
1787145268236386304 |
| score |
11.755432 |