Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae)
In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg/L were assayed in vitro against monogenoideans Anacanthorus spat...
| Autor principal: | Soares, Bruna Viana |
|---|---|
| Outros Autores: | Neves, Lígia Rigôr, Ferreira, Drielly Oliveira, Oliveira, Marcos Sidney Brito de, Chaves, Francisco Célio Maia, Chagas, Edsandra Campos, Gonçalves, Raissa Alves, Tavares-Dias, Marcos |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Veterinary Parasitology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17173 |
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oai:repositorio:1-17173 |
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oai:repositorio:1-17173 Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) Soares, Bruna Viana Neves, Lígia Rigôr Ferreira, Drielly Oliveira Oliveira, Marcos Sidney Brito de Chaves, Francisco Célio Maia Chagas, Edsandra Campos Gonçalves, Raissa Alves Tavares-Dias, Marcos Essential Oil Glucose Lippia Sidoides Extract Plant Extract Protein Unclassified Drug Anthelmintic Agent Essential Oil Plant Extract Anacanthorus Spathulatus Animals Experiment Animals Parasitosis Animals Tissue Antiprotozoal Activity Bleeding Cell Proliferation Colossoma Macropomum Controlled Study Edema Erythrocyte Count Gill Glucose Blood Level Histopathology Ichthyophthirius Multifiliis In Vitro Study In Vivo Study Lippia Lippia Sidoides Monogenea Mucous Cell Mymarothecium Boegeri Necrosis Nonhuman Notozothecium Janauachensis Protein Blood Level Vasodilatation Animals Characiformes Chemistry Drug Effects Erythrocyte Fish Diseases Parasitology Pathology Trematode Trematode Infections Veterinary Animal Anthelmintics Characiformes Erythrocytes Fish Diseases Gills Lippia Oils, Volatile Plant Extracts Trematoda Trematode Infections In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160 mg/L were 100% effective against monogenoideans in 10 min and 1 h of exposure, respectively. However, the effectiveness of 100% concentrations of 80 mg/L and 40 mg/L occurred in 3 and 6 h, respectively. In the in vivo tests, juvenile fish were submitted to 60 min of baths with 10 mg/L and 15 min of baths with 20 mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20 mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24 h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites. © 2016 Elsevier B.V. 2020-06-15T21:39:39Z 2020-06-15T21:39:39Z 2017 Artigo https://repositorio.inpa.gov.br/handle/1/17173 10.1016/j.vetpar.2016.12.012 en Volume 234, Pags. 49-56 Restrito Veterinary Parasitology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Essential Oil Glucose Lippia Sidoides Extract Plant Extract Protein Unclassified Drug Anthelmintic Agent Essential Oil Plant Extract Anacanthorus Spathulatus Animals Experiment Animals Parasitosis Animals Tissue Antiprotozoal Activity Bleeding Cell Proliferation Colossoma Macropomum Controlled Study Edema Erythrocyte Count Gill Glucose Blood Level Histopathology Ichthyophthirius Multifiliis In Vitro Study In Vivo Study Lippia Lippia Sidoides Monogenea Mucous Cell Mymarothecium Boegeri Necrosis Nonhuman Notozothecium Janauachensis Protein Blood Level Vasodilatation Animals Characiformes Chemistry Drug Effects Erythrocyte Fish Diseases Parasitology Pathology Trematode Trematode Infections Veterinary Animal Anthelmintics Characiformes Erythrocytes Fish Diseases Gills Lippia Oils, Volatile Plant Extracts Trematoda Trematode Infections |
| spellingShingle |
Essential Oil Glucose Lippia Sidoides Extract Plant Extract Protein Unclassified Drug Anthelmintic Agent Essential Oil Plant Extract Anacanthorus Spathulatus Animals Experiment Animals Parasitosis Animals Tissue Antiprotozoal Activity Bleeding Cell Proliferation Colossoma Macropomum Controlled Study Edema Erythrocyte Count Gill Glucose Blood Level Histopathology Ichthyophthirius Multifiliis In Vitro Study In Vivo Study Lippia Lippia Sidoides Monogenea Mucous Cell Mymarothecium Boegeri Necrosis Nonhuman Notozothecium Janauachensis Protein Blood Level Vasodilatation Animals Characiformes Chemistry Drug Effects Erythrocyte Fish Diseases Parasitology Pathology Trematode Trematode Infections Veterinary Animal Anthelmintics Characiformes Erythrocytes Fish Diseases Gills Lippia Oils, Volatile Plant Extracts Trematoda Trematode Infections Soares, Bruna Viana Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| topic_facet |
Essential Oil Glucose Lippia Sidoides Extract Plant Extract Protein Unclassified Drug Anthelmintic Agent Essential Oil Plant Extract Anacanthorus Spathulatus Animals Experiment Animals Parasitosis Animals Tissue Antiprotozoal Activity Bleeding Cell Proliferation Colossoma Macropomum Controlled Study Edema Erythrocyte Count Gill Glucose Blood Level Histopathology Ichthyophthirius Multifiliis In Vitro Study In Vivo Study Lippia Lippia Sidoides Monogenea Mucous Cell Mymarothecium Boegeri Necrosis Nonhuman Notozothecium Janauachensis Protein Blood Level Vasodilatation Animals Characiformes Chemistry Drug Effects Erythrocyte Fish Diseases Parasitology Pathology Trematode Trematode Infections Veterinary Animal Anthelmintics Characiformes Erythrocytes Fish Diseases Gills Lippia Oils, Volatile Plant Extracts Trematoda Trematode Infections |
| description |
In vivo and in vitro antiparasitic activity of the essential oil of Lippia sidoides and blood and histological alterations were assessed in Colossoma macropomum (tambaqui). Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg/L were assayed in vitro against monogenoideans Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri from fish gills. Lippia sidoides essential oil concentrations of 320 and 160 mg/L were 100% effective against monogenoideans in 10 min and 1 h of exposure, respectively. However, the effectiveness of 100% concentrations of 80 mg/L and 40 mg/L occurred in 3 and 6 h, respectively. In the in vivo tests, juvenile fish were submitted to 60 min of baths with 10 mg/L and 15 min of baths with 20 mg/L of the essential oil of L. sidoides. These therapeutic baths were not efficient against Ichthyophthirius multifiliis, and monogenoideans present in the gills of C. macropomum. In addition, 10 and 20 mg/L of the essential oil of L. sidoides caused an anesthetic effect on the fish and did not influence total glucose and protein plasma levels; however, it decreased the number of total erythrocytes in fish exposed to the higher concentration of this essential oil. Severe alterations and irreversible damage were observed in the fish gills just after L. sidoides essential oil baths and after 24 h of recovery. The most recurrent lesions found were hyperplasia and fusion of the lamellar epithelium, vasodilation, detachment of the gill epithelium and lamellar aneurism, epithelial breakdown with hemorrhage, congestion, edema and necrosis, proliferation of the mucous cells and chloride cells and lamellar hypertrophy. Therefore, since the essential oil of L. sidoides has in vitro antiparasitic activity and low concentrations of it have shown toxic effects, the bioactive potential of its main chemical components should be investigated, as well as more efficient forms of its administration in therapeutic baths in order to eliminate fish parasites. © 2016 Elsevier B.V. |
| format |
Artigo |
| author |
Soares, Bruna Viana |
| author2 |
Neves, Lígia Rigôr Ferreira, Drielly Oliveira Oliveira, Marcos Sidney Brito de Chaves, Francisco Célio Maia Chagas, Edsandra Campos Gonçalves, Raissa Alves Tavares-Dias, Marcos |
| author2Str |
Neves, Lígia Rigôr Ferreira, Drielly Oliveira Oliveira, Marcos Sidney Brito de Chaves, Francisco Célio Maia Chagas, Edsandra Campos Gonçalves, Raissa Alves Tavares-Dias, Marcos |
| title |
Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| title_short |
Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| title_full |
Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| title_fullStr |
Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| title_full_unstemmed |
Antiparasitic activity, histopathology and physiology of Colossoma macropomum (tambaqui) exposed to the essential oil of Lippia sidoides (Verbenaceae) |
| title_sort |
antiparasitic activity, histopathology and physiology of colossoma macropomum (tambaqui) exposed to the essential oil of lippia sidoides (verbenaceae) |
| publisher |
Veterinary Parasitology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17173 |
| _version_ |
1787142762156523520 |
| score |
11.755432 |