Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds o...
| Autor principal: | Calvi, Geangelo Petene |
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| Outros Autores: | Aud, Fabiana Ferraz, Ferraz, Isolde Dorothea Kossmann, Pritchard, Hugh W., Kranner, Ilse |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Plant Biology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17204 |
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oai:repositorio:1-17204 |
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dspace |
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oai:repositorio:1-17204 Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination Calvi, Geangelo Petene Aud, Fabiana Ferraz Ferraz, Isolde Dorothea Kossmann Pritchard, Hugh W. Kranner, Ilse Antioxidant Desiccation Dye Fruit Germination Growth Rate Morphology Oxidative Stress Seed Dormancy Seed Set Seedling Amazonia Antioxidant Biological Marker Glutathione Desiccation Eugenia Germination Metabolism Physiology Seed Plant Seedling Antioxidants Biomarkers Desiccation Eugenia Germination Glutathione Seedlings Seeds The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (γ-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG /2 GSH) was less negative than −215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG /2 GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (EC y SS /2 CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG /2 GSH values between −180 and −160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG /2 GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. © 2016 German Botanical Society and The Royal Botanical Society of the Netherlands 2020-06-15T21:39:57Z 2020-06-15T21:39:57Z 2017 Artigo https://repositorio.inpa.gov.br/handle/1/17204 10.1111/plb.12466 en Volume 19, Número 1, Pags. 6-13 Restrito Plant Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Antioxidant Desiccation Dye Fruit Germination Growth Rate Morphology Oxidative Stress Seed Dormancy Seed Set Seedling Amazonia Antioxidant Biological Marker Glutathione Desiccation Eugenia Germination Metabolism Physiology Seed Plant Seedling Antioxidants Biomarkers Desiccation Eugenia Germination Glutathione Seedlings Seeds |
| spellingShingle |
Antioxidant Desiccation Dye Fruit Germination Growth Rate Morphology Oxidative Stress Seed Dormancy Seed Set Seedling Amazonia Antioxidant Biological Marker Glutathione Desiccation Eugenia Germination Metabolism Physiology Seed Plant Seedling Antioxidants Biomarkers Desiccation Eugenia Germination Glutathione Seedlings Seeds Calvi, Geangelo Petene Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| topic_facet |
Antioxidant Desiccation Dye Fruit Germination Growth Rate Morphology Oxidative Stress Seed Dormancy Seed Set Seedling Amazonia Antioxidant Biological Marker Glutathione Desiccation Eugenia Germination Metabolism Physiology Seed Plant Seedling Antioxidants Biomarkers Desiccation Eugenia Germination Glutathione Seedlings Seeds |
| description |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (γ-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG /2 GSH) was less negative than −215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG /2 GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (EC y SS /2 CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG /2 GSH values between −180 and −160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG /2 GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. © 2016 German Botanical Society and The Royal Botanical Society of the Netherlands |
| format |
Artigo |
| author |
Calvi, Geangelo Petene |
| author2 |
Aud, Fabiana Ferraz Ferraz, Isolde Dorothea Kossmann Pritchard, Hugh W. Kranner, Ilse |
| author2Str |
Aud, Fabiana Ferraz Ferraz, Isolde Dorothea Kossmann Pritchard, Hugh W. Kranner, Ilse |
| title |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| title_short |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| title_full |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| title_fullStr |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| title_full_unstemmed |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination |
| title_sort |
analyses of several seed viability markers in individual recalcitrant seeds of eugenia stipitata mcvaugh with totipotent germination |
| publisher |
Plant Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17204 |
| _version_ |
1787141143385866240 |
| score |
11.755432 |