Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation
Filamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The iso...
| Autor principal: | Souza, Hilton Marcelo de Lima |
|---|---|
| Outros Autores: | Sette, Lara Duräes, Mota, Adolfo José da, Nascimento Neto, Joaquim Ferreira do, Rodrigues, A., Oliveira, T?ssio Brito de, Oliveira, Fernando Mendes de, Oliveira, Luiz Antonio de, Barroso, Hiléia dos Santos, Zanotto, Sandra Patricia |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Water, Air, and Soil Pollution
2020
|
| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17224 |
| id |
oai:repositorio:1-17224 |
|---|---|
| recordtype |
dspace |
| spelling |
oai:repositorio:1-17224 Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation Souza, Hilton Marcelo de Lima Sette, Lara Duräes Mota, Adolfo José da Nascimento Neto, Joaquim Ferreira do Rodrigues, A. Oliveira, T?ssio Brito de Oliveira, Fernando Mendes de Oliveira, Luiz Antonio de Barroso, Hiléia dos Santos Zanotto, Sandra Patricia Aromatic Compounds Aromatic Hydrocarbons Biodegradability Dyes Enzymes Fungi Gas Chromatography Hydrocarbons Mass Spectrometry Polycyclic Aromatic Hydrocarbons Pyrene Benzo(a)pyrene Biodegradability Tests Carcinogenic Properties Gas Chromatography Coupled To Mass Spectrometries (gc Ms) High Molecular Weight Ligninolytic Enzymes Polycyclic Aromatic Hydrocarbon (pah) Taxonomic Identifications Biodegradation 2,6 Dichlorophenolindophenol Benzo(a)pyrene Gallic Acid Laccase Manganese Peroxidase Biodegradation Enzyme Activity Fungus Genetic Resources Pah Sediment Pollution Biodegradation Brasil Controlled Study Enzyme Activity Filamentous Fungus Mass Fragmentography Megasporoporia Nonhuman Oxidation Reduction State Sediment Sordariales Water Contamination Amazonia Brasil Fungi Megasporoporia Sordariales Filamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization. © 2016, Springer International Publishing Switzerland. 2020-06-15T21:40:07Z 2020-06-15T21:40:07Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/17224 10.1007/s11270-016-3101-y en Volume 227, Número 12 Restrito Water, Air, and Soil Pollution |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Aromatic Compounds Aromatic Hydrocarbons Biodegradability Dyes Enzymes Fungi Gas Chromatography Hydrocarbons Mass Spectrometry Polycyclic Aromatic Hydrocarbons Pyrene Benzo(a)pyrene Biodegradability Tests Carcinogenic Properties Gas Chromatography Coupled To Mass Spectrometries (gc Ms) High Molecular Weight Ligninolytic Enzymes Polycyclic Aromatic Hydrocarbon (pah) Taxonomic Identifications Biodegradation 2,6 Dichlorophenolindophenol Benzo(a)pyrene Gallic Acid Laccase Manganese Peroxidase Biodegradation Enzyme Activity Fungus Genetic Resources Pah Sediment Pollution Biodegradation Brasil Controlled Study Enzyme Activity Filamentous Fungus Mass Fragmentography Megasporoporia Nonhuman Oxidation Reduction State Sediment Sordariales Water Contamination Amazonia Brasil Fungi Megasporoporia Sordariales |
| spellingShingle |
Aromatic Compounds Aromatic Hydrocarbons Biodegradability Dyes Enzymes Fungi Gas Chromatography Hydrocarbons Mass Spectrometry Polycyclic Aromatic Hydrocarbons Pyrene Benzo(a)pyrene Biodegradability Tests Carcinogenic Properties Gas Chromatography Coupled To Mass Spectrometries (gc Ms) High Molecular Weight Ligninolytic Enzymes Polycyclic Aromatic Hydrocarbon (pah) Taxonomic Identifications Biodegradation 2,6 Dichlorophenolindophenol Benzo(a)pyrene Gallic Acid Laccase Manganese Peroxidase Biodegradation Enzyme Activity Fungus Genetic Resources Pah Sediment Pollution Biodegradation Brasil Controlled Study Enzyme Activity Filamentous Fungus Mass Fragmentography Megasporoporia Nonhuman Oxidation Reduction State Sediment Sordariales Water Contamination Amazonia Brasil Fungi Megasporoporia Sordariales Souza, Hilton Marcelo de Lima Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| topic_facet |
Aromatic Compounds Aromatic Hydrocarbons Biodegradability Dyes Enzymes Fungi Gas Chromatography Hydrocarbons Mass Spectrometry Polycyclic Aromatic Hydrocarbons Pyrene Benzo(a)pyrene Biodegradability Tests Carcinogenic Properties Gas Chromatography Coupled To Mass Spectrometries (gc Ms) High Molecular Weight Ligninolytic Enzymes Polycyclic Aromatic Hydrocarbon (pah) Taxonomic Identifications Biodegradation 2,6 Dichlorophenolindophenol Benzo(a)pyrene Gallic Acid Laccase Manganese Peroxidase Biodegradation Enzyme Activity Fungus Genetic Resources Pah Sediment Pollution Biodegradation Brasil Controlled Study Enzyme Activity Filamentous Fungus Mass Fragmentography Megasporoporia Nonhuman Oxidation Reduction State Sediment Sordariales Water Contamination Amazonia Brasil Fungi Megasporoporia Sordariales |
| description |
Filamentous fungi were isolated from contaminated sediment samples in the Amazon region of Brazil to select species with potential for benzo(a)pyrene (BaP) degradation, a polycyclic aromatic hydrocarbon (PAH) with high molecular weight and known for its mutagenic and carcinogenic properties. The isolates were submitted to biodegradability test using 2.6-dichlorophenol indophenol (DCPIP), gallic acid reaction, and evaluation of BaP influence in the enzymatic (ligninolytic) activity. The selected fungi were submitted to the taxonomic identification and used in biodegradation assays, which were carried out using gas chromatography coupled to mass spectrometry (GC-MS). A qualitative analysis of the presence of BaP metabolites that have recently been reported in literature was also performed by GC-MS. A total of 146 fungal isolates were recovered. Among them, 63.7 % were positive for the redox indicator DCPIP. From these isolates, 22.6 % showed positive responses to the gallic acid reaction. In enzymatic tests, the fungi Megasporoporia sp. S47 and unidentified Sordariales S69 presented highest activities of laccase and manganese peroxidase in the presence of BaP. Additionally, the white-rot fungus Megasporoporia sp. S47 showed better performance in BaP degradation (54 %). Therefore, Megasporoporia sp. S47, obtained from an environment with considerable PAH contamination, was selected as a promising genetic resource for application in new studies related to enzyme production and characterization and BaP degradation optimization. © 2016, Springer International Publishing Switzerland. |
| format |
Artigo |
| author |
Souza, Hilton Marcelo de Lima |
| author2 |
Sette, Lara Duräes Mota, Adolfo José da Nascimento Neto, Joaquim Ferreira do Rodrigues, A. Oliveira, T?ssio Brito de Oliveira, Fernando Mendes de Oliveira, Luiz Antonio de Barroso, Hiléia dos Santos Zanotto, Sandra Patricia |
| author2Str |
Sette, Lara Duräes Mota, Adolfo José da Nascimento Neto, Joaquim Ferreira do Rodrigues, A. Oliveira, T?ssio Brito de Oliveira, Fernando Mendes de Oliveira, Luiz Antonio de Barroso, Hiléia dos Santos Zanotto, Sandra Patricia |
| title |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| title_short |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| title_full |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| title_fullStr |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| title_full_unstemmed |
Filamentous Fungi Isolates of Contaminated Sediment in the Amazon Region with the Potential for Benzo(a)pyrene Degradation |
| title_sort |
filamentous fungi isolates of contaminated sediment in the amazon region with the potential for benzo(a)pyrene degradation |
| publisher |
Water, Air, and Soil Pollution |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17224 |
| _version_ |
1787141776566386688 |
| score |
11.755432 |