An RNA-based approach to sequence the mitogenome of Hypoptopoma incognitum (Siluriformes: Loricariidae)
Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the c...
| Autor principal: | Moreira, Daniel Andrade |
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| Outros Autores: | Magalhães, Maithê Gaspar Pontes, Andrade, Paula C.C. de, Furtado, Carolina, Val, Adalberto Luis, Parente, Thiago E.M. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Mitochondrial DNA
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17267 |
| Resumo: |
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Hypoptopoma incognitum is a fish of the fifth most species-rich family of vertebrates and abundant in rivers from the Brazilian Amazon. Only two species of Loricariidae fish have their complete mitogenomes sequence deposited in the Genbank. An innovative RNA-based approach was used to assemble the complete mitogenome of H. incognitum with an average coverage depth of 5292×. The typical vertebrate mitochondrial features were found; 22 tRNA genes, two rRNA genes, 13 protein-coding genes, and a non-coding control region. Moreover, the use of this approach allowed the measurement of mtRNA expression levels, the punctuation pattern of editing, and the detection of heteroplasmies. © 2015 Taylor & Francis. |