Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model

Silva, Francijara Araújo da; Schneider, Carlos Henrique; Feldberg, Eliana; Baccaro, Fabricio Beggiato; Carvalho, Natália Dayane Moura; Gross, Maria Claudia

Artigo

Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model

The Amazon has abundant rivers, streams, and floodplains in both polluted and nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences in both mitotic chromosomes and erythrocyte micronuclei of tamoatás from polluted and nonpolluted en...

ver descrição completa

Autor principal:	Silva, Francijara Araújo da
Outros Autores:	Schneider, Carlos Henrique, Feldberg, Eliana, Baccaro, Fabricio Beggiato, Carvalho, Natália Dayane Moura, Gross, Maria Claudia
Grau:	Artigo
Idioma:	English
Publicado em:	Zebrafish 2020
Assuntos:	Ribosome Dna Rna 18s Rna 5s Animals Catfish Chromosomal Mapping Ecosystem Epigenesis, Genetic Genetics Genomics Mitosis River Animal Catfishes Chromosome Mapping Ribosomal Dna Ecosystem Epigenesis, Genetic Genomics Mitosis Rivers Rna, Ribosomal, 18s Rna, Ribosomal, 5s
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/17313

id	oai:repositorio:1-17313
recordtype	dspace
spelling	oai:repositorio:1-17313 Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model Silva, Francijara Araújo da Schneider, Carlos Henrique Feldberg, Eliana Baccaro, Fabricio Beggiato Carvalho, Natália Dayane Moura Gross, Maria Claudia Ribosome Dna Rna 18s Rna 5s Animals Catfish Chromosomal Mapping Ecosystem Epigenesis, Genetic Genetics Genomics Mitosis River Animal Catfishes Chromosome Mapping Ribosomal Dna Ecosystem Epigenesis, Genetic Genomics Mitosis Rivers Rna, Ribosomal, 18s Rna, Ribosomal, 5s The Amazon has abundant rivers, streams, and floodplains in both polluted and nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences in both mitotic chromosomes and erythrocyte micronuclei of tamoatás from polluted and nonpolluted environments and to assess the possible genotoxic effects of these environments. Individuals were collected in Manaus, Amazonas (AM), and submitted to classical and molecular cytogenetic techniques, as well as to a blood micronucleus test. Diploid number equal to 60 chromosomes are present in all individuals, with 18S ribosomal DNA sites present in one chromosome pair and no interstitial telomeric sites on chromosomes. The micronucleus test showed no significant differences in pairwise comparisons between environments or collection sites, but the Rex3 retroelement was dispersed on the chromosomes of individuals from unpolluted environments and compartmentalized in individuals from polluted environments. Divergent numbers of 5S rDNA sites are present in individuals from unpolluted and polluted environments. The mapping of repetitive sequences revealed that micronuclei have different compositions both intra- and interindividually that suggests different regions are lost in the formation of micronuclei, and no single fragile region undergoes breaks, although repetitive DNA elements are involved in this process. © Copyright 2016, Mary Ann Liebert, Inc. 2016. 2020-06-15T21:41:08Z 2020-06-15T21:41:08Z 2016 Artigo https://repositorio.inpa.gov.br/handle/1/17313 10.1089/zeb.2015.1237 en Volume 13, Número 3, Pags. 197-208 Restrito Zebrafish
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Ribosome Dna Rna 18s Rna 5s Animals Catfish Chromosomal Mapping Ecosystem Epigenesis, Genetic Genetics Genomics Mitosis River Animal Catfishes Chromosome Mapping Ribosomal Dna Ecosystem Epigenesis, Genetic Genomics Mitosis Rivers Rna, Ribosomal, 18s Rna, Ribosomal, 5s
spellingShingle	Ribosome Dna Rna 18s Rna 5s Animals Catfish Chromosomal Mapping Ecosystem Epigenesis, Genetic Genetics Genomics Mitosis River Animal Catfishes Chromosome Mapping Ribosomal Dna Ecosystem Epigenesis, Genetic Genomics Mitosis Rivers Rna, Ribosomal, 18s Rna, Ribosomal, 5s Silva, Francijara Araújo da Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
topic_facet	Ribosome Dna Rna 18s Rna 5s Animals Catfish Chromosomal Mapping Ecosystem Epigenesis, Genetic Genetics Genomics Mitosis River Animal Catfishes Chromosome Mapping Ribosomal Dna Ecosystem Epigenesis, Genetic Genomics Mitosis Rivers Rna, Ribosomal, 18s Rna, Ribosomal, 5s
description	The Amazon has abundant rivers, streams, and floodplains in both polluted and nonpolluted environments, which show great adaptability. Thus, the goal of this study was to map repetitive DNA sequences in both mitotic chromosomes and erythrocyte micronuclei of tamoatás from polluted and nonpolluted environments and to assess the possible genotoxic effects of these environments. Individuals were collected in Manaus, Amazonas (AM), and submitted to classical and molecular cytogenetic techniques, as well as to a blood micronucleus test. Diploid number equal to 60 chromosomes are present in all individuals, with 18S ribosomal DNA sites present in one chromosome pair and no interstitial telomeric sites on chromosomes. The micronucleus test showed no significant differences in pairwise comparisons between environments or collection sites, but the Rex3 retroelement was dispersed on the chromosomes of individuals from unpolluted environments and compartmentalized in individuals from polluted environments. Divergent numbers of 5S rDNA sites are present in individuals from unpolluted and polluted environments. The mapping of repetitive sequences revealed that micronuclei have different compositions both intra- and interindividually that suggests different regions are lost in the formation of micronuclei, and no single fragile region undergoes breaks, although repetitive DNA elements are involved in this process. © Copyright 2016, Mary Ann Liebert, Inc. 2016.
format	Artigo
author	Silva, Francijara Araújo da
author2	Schneider, Carlos Henrique Feldberg, Eliana Baccaro, Fabricio Beggiato Carvalho, Natália Dayane Moura Gross, Maria Claudia
author2Str	Schneider, Carlos Henrique Feldberg, Eliana Baccaro, Fabricio Beggiato Carvalho, Natália Dayane Moura Gross, Maria Claudia
title	Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
title_short	Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
title_full	Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
title_fullStr	Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
title_full_unstemmed	Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model
title_sort	genomic organization under different environmental conditions: hoplosternum littorale as a model
publisher	Zebrafish
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/17313
_version_	1787145424339992576
score	11.653393

Genomic Organization under Different Environmental Conditions: Hoplosternum Littorale as a Model

Registros relacionados