Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon
Lutzomyia (Nyssomyia) anduzei has been recognized as a secondary vector of Leishmania guyanensis in the Brazilian Amazon region. Since L. anduzei is anthropophilic, co-exists in areas of high leishmaniasis transmission and has been found infected with L. guyanensis, the understanding of the vector p...
| Autor principal: | Scarpassa, Vera Margarete |
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| Outros Autores: | Figueiredo, Adrya da Silva, Alencar, Ronildo Baiatone |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Infection, Genetics and Evolution
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17485 |
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oai:repositorio:1-17485 |
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oai:repositorio:1-17485 Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon Scarpassa, Vera Margarete Figueiredo, Adrya da Silva Alencar, Ronildo Baiatone Microorganism Protein Dna, Mitochondrial Brasil Coi Gene Controlled Study Cytb Gene Gene Gene Flow Gene Sequence Genetic Variability Habitat Fragmentation Haplotype Leishmania Lutzomyia Anduzei Nonhuman Phylogeography Population Dispersal Population Structure Priority Journal Psychodidae Species Habitat Vector Control Animals Classification Diptera Disease Carrier Gene Frequency Genetics Geography Leishmania Guyanensis Mitochondrial Gene Parasitology Genetics, Population Diptera Leishmania Guyanensis Nyssomyia Phlebotominae Psychodidae Animal Brasil Diptera Gene Frequency Genes, Mitochondrial Genetic Variation Genetics, Population Geography Haplotypes Insect Vectors Leishmania Guyanensis Lutzomyia (Nyssomyia) anduzei has been recognized as a secondary vector of Leishmania guyanensis in the Brazilian Amazon region. Since L. anduzei is anthropophilic, co-exists in areas of high leishmaniasis transmission and has been found infected with L. guyanensis, the understanding of the vector population structure and of the process responsible for it is paramount to the vector management and control efforts. In this study we analyzed 74 and 67 sequences of the COI and Cytb loci, respectively, from mitochondrial DNA, aiming to estimate the intra-population genetic variability and population structure in six L. anduzei samples from the Brazilian Amazon region. For COI, we found 58 haplotypes, low to high (FST=0.0310-0.4128) and significant (P=0.0033) genetic structure, and reduced gene flow among populations. The haplotype network yielded many reticulations that likely resulted from hypervariability in the locus. For Cytb, we observed 27 haplotypes, low to moderate (FST=0.0077-0.1954) and nonsignificant (P>0.05) genetic structure for the majority of comparisons and extensive gene flow among populations, in line with the haplotypes network data. AMOVA analysis indicated that most of the variation occurred within populations (83.41%, 90.94%); nevertheless, there were significant differences (ΦST=0.0906-0.1659; P=0.00098; P=0.00000) among them for both loci. The Mantel test showed that the genetic structure is not associated to an isolation-by-distance (IBD) model in either of both loci. These data suggest that L. anduzei is genetically very diverse. The genetic structure lacking IBD may be due to adaptation to local habitats and the low dispersal capacity of the sandflies, and both could lead to population fragmentation and geographic isolation. These findings have important implications for epidemiology, surveillance and vector control and may be a first step in understanding the evolutionary history of this species. © 2015 Elsevier B.V. 2020-06-15T21:48:03Z 2020-06-15T21:48:03Z 2015 Artigo https://repositorio.inpa.gov.br/handle/1/17485 10.1016/j.meegid.2015.02.007 en Volume 31, Pags. 312-320 Restrito Infection, Genetics and Evolution |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Microorganism Protein Dna, Mitochondrial Brasil Coi Gene Controlled Study Cytb Gene Gene Gene Flow Gene Sequence Genetic Variability Habitat Fragmentation Haplotype Leishmania Lutzomyia Anduzei Nonhuman Phylogeography Population Dispersal Population Structure Priority Journal Psychodidae Species Habitat Vector Control Animals Classification Diptera Disease Carrier Gene Frequency Genetics Geography Leishmania Guyanensis Mitochondrial Gene Parasitology Genetics, Population Diptera Leishmania Guyanensis Nyssomyia Phlebotominae Psychodidae Animal Brasil Diptera Gene Frequency Genes, Mitochondrial Genetic Variation Genetics, Population Geography Haplotypes Insect Vectors Leishmania Guyanensis |
| spellingShingle |
Microorganism Protein Dna, Mitochondrial Brasil Coi Gene Controlled Study Cytb Gene Gene Gene Flow Gene Sequence Genetic Variability Habitat Fragmentation Haplotype Leishmania Lutzomyia Anduzei Nonhuman Phylogeography Population Dispersal Population Structure Priority Journal Psychodidae Species Habitat Vector Control Animals Classification Diptera Disease Carrier Gene Frequency Genetics Geography Leishmania Guyanensis Mitochondrial Gene Parasitology Genetics, Population Diptera Leishmania Guyanensis Nyssomyia Phlebotominae Psychodidae Animal Brasil Diptera Gene Frequency Genes, Mitochondrial Genetic Variation Genetics, Population Geography Haplotypes Insect Vectors Leishmania Guyanensis Scarpassa, Vera Margarete Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| topic_facet |
Microorganism Protein Dna, Mitochondrial Brasil Coi Gene Controlled Study Cytb Gene Gene Gene Flow Gene Sequence Genetic Variability Habitat Fragmentation Haplotype Leishmania Lutzomyia Anduzei Nonhuman Phylogeography Population Dispersal Population Structure Priority Journal Psychodidae Species Habitat Vector Control Animals Classification Diptera Disease Carrier Gene Frequency Genetics Geography Leishmania Guyanensis Mitochondrial Gene Parasitology Genetics, Population Diptera Leishmania Guyanensis Nyssomyia Phlebotominae Psychodidae Animal Brasil Diptera Gene Frequency Genes, Mitochondrial Genetic Variation Genetics, Population Geography Haplotypes Insect Vectors Leishmania Guyanensis |
| description |
Lutzomyia (Nyssomyia) anduzei has been recognized as a secondary vector of Leishmania guyanensis in the Brazilian Amazon region. Since L. anduzei is anthropophilic, co-exists in areas of high leishmaniasis transmission and has been found infected with L. guyanensis, the understanding of the vector population structure and of the process responsible for it is paramount to the vector management and control efforts. In this study we analyzed 74 and 67 sequences of the COI and Cytb loci, respectively, from mitochondrial DNA, aiming to estimate the intra-population genetic variability and population structure in six L. anduzei samples from the Brazilian Amazon region. For COI, we found 58 haplotypes, low to high (FST=0.0310-0.4128) and significant (P=0.0033) genetic structure, and reduced gene flow among populations. The haplotype network yielded many reticulations that likely resulted from hypervariability in the locus. For Cytb, we observed 27 haplotypes, low to moderate (FST=0.0077-0.1954) and nonsignificant (P>0.05) genetic structure for the majority of comparisons and extensive gene flow among populations, in line with the haplotypes network data. AMOVA analysis indicated that most of the variation occurred within populations (83.41%, 90.94%); nevertheless, there were significant differences (ΦST=0.0906-0.1659; P=0.00098; P=0.00000) among them for both loci. The Mantel test showed that the genetic structure is not associated to an isolation-by-distance (IBD) model in either of both loci. These data suggest that L. anduzei is genetically very diverse. The genetic structure lacking IBD may be due to adaptation to local habitats and the low dispersal capacity of the sandflies, and both could lead to population fragmentation and geographic isolation. These findings have important implications for epidemiology, surveillance and vector control and may be a first step in understanding the evolutionary history of this species. © 2015 Elsevier B.V. |
| format |
Artigo |
| author |
Scarpassa, Vera Margarete |
| author2 |
Figueiredo, Adrya da Silva Alencar, Ronildo Baiatone |
| author2Str |
Figueiredo, Adrya da Silva Alencar, Ronildo Baiatone |
| title |
Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| title_short |
Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| title_full |
Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| title_fullStr |
Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| title_full_unstemmed |
Genetic diversity and population structure in the Leishmania guyanensis vector Lutzomyia anduzei (Diptera, Psychodidae) from the Brazilian Amazon |
| title_sort |
genetic diversity and population structure in the leishmania guyanensis vector lutzomyia anduzei (diptera, psychodidae) from the brazilian amazon |
| publisher |
Infection, Genetics and Evolution |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17485 |
| _version_ |
1787142428241690624 |
| score |
11.755432 |