Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus
The possible origins and differentiation of a ZZ/ZW sex chromosome system in Semaprochilodus taeniurus, the only species of the family Prochilodontidae known to possess heteromorphic sex chromosomes, were examined by conventional (C-banding) and molecular (cross-species hybridization of W-specific W...
| Autor principal: | Terêncio, Maria Leandra |
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| Outros Autores: | Schneider, Carlos Henrique, Gross, Maria Claudia, Nogaroto, Viviane, Almeida, Mara Cristina de, Artoni, Roberto Ferreira, Vicari, Marcelo Ricardo, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetica
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/17960 |
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oai:repositorio:1-17960 |
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oai:repositorio:1-17960 Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus Terêncio, Maria Leandra Schneider, Carlos Henrique Gross, Maria Claudia Nogaroto, Viviane Almeida, Mara Cristina de Artoni, Roberto Ferreira Vicari, Marcelo Ricardo Feldberg, Eliana Dna Animals Characiformes Chromosome Painting Dna Sequence Female In Situ Hybridization, Fluorescence Genetics Genomics Heterochromatin Image Processing Isolation And Purification Male Methodology Cloning, Molecular Nucleotide Repeat Sex Chromosome Telomere Animal Characiformes Chromosome Painting Cloning, Molecular Dna Female Genomics Heterochromatin Image Processing, Computer-assisted In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Sequence Analysis, Dna Sex Chromosomes Telomere Prochilodontidae Prochilodus Lineatus Semaprochilodus Taeniurus The possible origins and differentiation of a ZZ/ZW sex chromosome system in Semaprochilodus taeniurus, the only species of the family Prochilodontidae known to possess heteromorphic sex chromosomes, were examined by conventional (C-banding) and molecular (cross-species hybridization of W-specific WCP, Fluorescence in situ hybridization (FISH) with telomere (TTAGGG)n, and Rex1 probes) cytogenetic protocols. Several segments obtained by W-specific probe were cloned, and the sequences localized on the W chromosome were identified by DNA sequencing and search of nucleotide collections of the NCBI and GIRI using BLAST and CENSOR, respectively. Blocks of constitutive heterochromatin in chromosomes of S. taeniurus were observed in the centromere of all autosomal chromosomes and in the terminal, interstitial, and pericentromeric regions of the W chromosome, which did not demonstrate interstitial telomeric sites with FISH of the telomere probe. The Rex1 probe displayed a compartmentalized distribution pattern in some chromosomes and showed signs of invasion of the pericentromeric region in the W chromosome. Chromosomal painting with the W-specific WCP of S. taeniurus onto its own chromosomes showed complete staining of the W chromosome, centromeric sites, and the ends of the Z chromosome, as well as other autosomes. However, cross-species painting using this WCP on chromosomes of S. insignis, Prochilodus lineatus, and P. nigricans did not reveal a proto-W element, but instead demonstrated scattered positive signals of repetitive DNAs. Identification of the W-specific repetitive sequences showed high similarity to microsatellites and transposable elements. Classes of repetitive DNA identified in the W chromosome suggested that the genetic degeneration of this chromosome in S. taeniurus occurred through accumulation of these repetitive DNAs. © 2013 Springer Science+Business Media Dordrecht. 2020-06-15T21:50:28Z 2020-06-15T21:50:28Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/17960 10.1007/s10709-013-9699-4 en Volume 140, Número 10-12, Pags. 505-512 Restrito Genetica |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna Animals Characiformes Chromosome Painting Dna Sequence Female In Situ Hybridization, Fluorescence Genetics Genomics Heterochromatin Image Processing Isolation And Purification Male Methodology Cloning, Molecular Nucleotide Repeat Sex Chromosome Telomere Animal Characiformes Chromosome Painting Cloning, Molecular Dna Female Genomics Heterochromatin Image Processing, Computer-assisted In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Sequence Analysis, Dna Sex Chromosomes Telomere Prochilodontidae Prochilodus Lineatus Semaprochilodus Taeniurus |
| spellingShingle |
Dna Animals Characiformes Chromosome Painting Dna Sequence Female In Situ Hybridization, Fluorescence Genetics Genomics Heterochromatin Image Processing Isolation And Purification Male Methodology Cloning, Molecular Nucleotide Repeat Sex Chromosome Telomere Animal Characiformes Chromosome Painting Cloning, Molecular Dna Female Genomics Heterochromatin Image Processing, Computer-assisted In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Sequence Analysis, Dna Sex Chromosomes Telomere Prochilodontidae Prochilodus Lineatus Semaprochilodus Taeniurus Terêncio, Maria Leandra Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| topic_facet |
Dna Animals Characiformes Chromosome Painting Dna Sequence Female In Situ Hybridization, Fluorescence Genetics Genomics Heterochromatin Image Processing Isolation And Purification Male Methodology Cloning, Molecular Nucleotide Repeat Sex Chromosome Telomere Animal Characiformes Chromosome Painting Cloning, Molecular Dna Female Genomics Heterochromatin Image Processing, Computer-assisted In Situ Hybridization, Fluorescence Male Repetitive Sequences, Nucleic Acid Sequence Analysis, Dna Sex Chromosomes Telomere Prochilodontidae Prochilodus Lineatus Semaprochilodus Taeniurus |
| description |
The possible origins and differentiation of a ZZ/ZW sex chromosome system in Semaprochilodus taeniurus, the only species of the family Prochilodontidae known to possess heteromorphic sex chromosomes, were examined by conventional (C-banding) and molecular (cross-species hybridization of W-specific WCP, Fluorescence in situ hybridization (FISH) with telomere (TTAGGG)n, and Rex1 probes) cytogenetic protocols. Several segments obtained by W-specific probe were cloned, and the sequences localized on the W chromosome were identified by DNA sequencing and search of nucleotide collections of the NCBI and GIRI using BLAST and CENSOR, respectively. Blocks of constitutive heterochromatin in chromosomes of S. taeniurus were observed in the centromere of all autosomal chromosomes and in the terminal, interstitial, and pericentromeric regions of the W chromosome, which did not demonstrate interstitial telomeric sites with FISH of the telomere probe. The Rex1 probe displayed a compartmentalized distribution pattern in some chromosomes and showed signs of invasion of the pericentromeric region in the W chromosome. Chromosomal painting with the W-specific WCP of S. taeniurus onto its own chromosomes showed complete staining of the W chromosome, centromeric sites, and the ends of the Z chromosome, as well as other autosomes. However, cross-species painting using this WCP on chromosomes of S. insignis, Prochilodus lineatus, and P. nigricans did not reveal a proto-W element, but instead demonstrated scattered positive signals of repetitive DNAs. Identification of the W-specific repetitive sequences showed high similarity to microsatellites and transposable elements. Classes of repetitive DNA identified in the W chromosome suggested that the genetic degeneration of this chromosome in S. taeniurus occurred through accumulation of these repetitive DNAs. © 2013 Springer Science+Business Media Dordrecht. |
| format |
Artigo |
| author |
Terêncio, Maria Leandra |
| author2 |
Schneider, Carlos Henrique Gross, Maria Claudia Nogaroto, Viviane Almeida, Mara Cristina de Artoni, Roberto Ferreira Vicari, Marcelo Ricardo Feldberg, Eliana |
| author2Str |
Schneider, Carlos Henrique Gross, Maria Claudia Nogaroto, Viviane Almeida, Mara Cristina de Artoni, Roberto Ferreira Vicari, Marcelo Ricardo Feldberg, Eliana |
| title |
Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| title_short |
Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| title_full |
Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| title_fullStr |
Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| title_full_unstemmed |
Repetitive sequences associated with differentiation of W chromosome in Semaprochilodus taeniurus |
| title_sort |
repetitive sequences associated with differentiation of w chromosome in semaprochilodus taeniurus |
| publisher |
Genetica |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/17960 |
| _version_ |
1787143360716210176 |
| score |
11.755432 |