Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis
Classical and molecular cytogenetic (18S rDNA, telomeric sequence, and LINE-1 retrotransposon probes) studies were carried out to contribute to an understanding of the organization of repeated DNA elements in the Amazon River dolphin (boto, Inia geoffrensis). Twenty-seven specimens were examined, ea...
| Autor principal: | Bonifácio, Heidi L. |
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| Outros Autores: | Silva, Vera Maria Ferreira da, Martin, Anthony Richard, Feldberg, Eliana |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Genetica
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18001 |
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oai:repositorio:1-18001 |
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dspace |
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oai:repositorio:1-18001 Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis Bonifácio, Heidi L. Silva, Vera Maria Ferreira da Martin, Anthony Richard Feldberg, Eliana Ribosome Dna Animals Chromosome Nor Cytogenetics Dolphin Euchromatin Genetics Karyotyping Metabolism River Animal Cytogenetics Ribosomal Dna Dolphins Euchromatin Karyotyping Nucleolus Organizer Region Rivers Cetacea Inia Geoffrensis Classical and molecular cytogenetic (18S rDNA, telomeric sequence, and LINE-1 retrotransposon probes) studies were carried out to contribute to an understanding of the organization of repeated DNA elements in the Amazon River dolphin (boto, Inia geoffrensis). Twenty-seven specimens were examined, each presenting 2n = 44 chromosomes, the karyotype formula 12m + 14sm + 6st + 10t + XX/XY, and fundamental number (FN) = 74. C-positive heterochromatin was observed in terminal and interstitial positions, with the occurrence of polymorphism. Interstitial telomeric sequences were not observed. The nucleolar organizer region (NOR) was located at a single site on a smallest autosomal pair. LINE-1 was preferentially distributed in the euchromatin regions, with the greatest accumulation on the X chromosome. Although the karyotype structure in cetaceans is considered to be conserved, the boto karyotype demonstrated significant variations in its formula, heterochromatin distribution, and the location of the NOR compared to other cetacean species. These results contribute to knowledge of the chromosome organization in boto and to a better understanding of karyoevolution in cetaceans. © 2012 Springer Science+Business Media B.V. 2020-06-15T21:50:49Z 2020-06-15T21:50:49Z 2012 Artigo https://repositorio.inpa.gov.br/handle/1/18001 10.1007/s10709-012-9680-7 en Volume 140, Número 7-9, Pags. 307-315 Restrito Genetica |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Ribosome Dna Animals Chromosome Nor Cytogenetics Dolphin Euchromatin Genetics Karyotyping Metabolism River Animal Cytogenetics Ribosomal Dna Dolphins Euchromatin Karyotyping Nucleolus Organizer Region Rivers Cetacea Inia Geoffrensis |
| spellingShingle |
Ribosome Dna Animals Chromosome Nor Cytogenetics Dolphin Euchromatin Genetics Karyotyping Metabolism River Animal Cytogenetics Ribosomal Dna Dolphins Euchromatin Karyotyping Nucleolus Organizer Region Rivers Cetacea Inia Geoffrensis Bonifácio, Heidi L. Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| topic_facet |
Ribosome Dna Animals Chromosome Nor Cytogenetics Dolphin Euchromatin Genetics Karyotyping Metabolism River Animal Cytogenetics Ribosomal Dna Dolphins Euchromatin Karyotyping Nucleolus Organizer Region Rivers Cetacea Inia Geoffrensis |
| description |
Classical and molecular cytogenetic (18S rDNA, telomeric sequence, and LINE-1 retrotransposon probes) studies were carried out to contribute to an understanding of the organization of repeated DNA elements in the Amazon River dolphin (boto, Inia geoffrensis). Twenty-seven specimens were examined, each presenting 2n = 44 chromosomes, the karyotype formula 12m + 14sm + 6st + 10t + XX/XY, and fundamental number (FN) = 74. C-positive heterochromatin was observed in terminal and interstitial positions, with the occurrence of polymorphism. Interstitial telomeric sequences were not observed. The nucleolar organizer region (NOR) was located at a single site on a smallest autosomal pair. LINE-1 was preferentially distributed in the euchromatin regions, with the greatest accumulation on the X chromosome. Although the karyotype structure in cetaceans is considered to be conserved, the boto karyotype demonstrated significant variations in its formula, heterochromatin distribution, and the location of the NOR compared to other cetacean species. These results contribute to knowledge of the chromosome organization in boto and to a better understanding of karyoevolution in cetaceans. © 2012 Springer Science+Business Media B.V. |
| format |
Artigo |
| author |
Bonifácio, Heidi L. |
| author2 |
Silva, Vera Maria Ferreira da Martin, Anthony Richard Feldberg, Eliana |
| author2Str |
Silva, Vera Maria Ferreira da Martin, Anthony Richard Feldberg, Eliana |
| title |
Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| title_short |
Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| title_full |
Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| title_fullStr |
Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| title_full_unstemmed |
Molecular cytogenetic characterization of the Amazon River dolphin Inia geoffrensis |
| title_sort |
molecular cytogenetic characterization of the amazon river dolphin inia geoffrensis |
| publisher |
Genetica |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18001 |
| _version_ |
1787143933929717760 |
| score |
11.674684 |