Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil
The freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high n...
| Autor principal: | Silva, M. R. da |
|---|---|
| Outros Autores: | Matoso, D. A., Ludwig, Luis Augusto M., Gomes, Eder, Almeida, Mara Cristina de, Vicari, Marcelo Ricardo, Artoni, Roberto Ferreira |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Environmental Biology of Fishes
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18174 |
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oai:repositorio:1-18174 |
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oai:repositorio:1-18174 Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil Silva, M. R. da Matoso, D. A. Ludwig, Luis Augusto M. Gomes, Eder Almeida, Mara Cristina de Vicari, Marcelo Ricardo Artoni, Roberto Ferreira Captivity Chromosome Conservation Genetics Cytogenetics Genetic Marker Geographical Distribution Hybridization Molecular Analysis Monitoring System Native Species Ploidy Probe Sterility Teleost Visualization Brasil Iguacu Basin Rhamdia Rhamdia Quelen The freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high number of triploid specimens (46.8%) in the samples analyzed. The use of double fluorescent in situ hybridization (double-FISH) with 18S and 5S rDNA probes allowed highlighting the location of ribosomal sites with independent activity. This chromosome marker allowed the identification of triploid nuclei, in which three markers were visualized, in contrast to diploid fish, which only exhibited two fluorescent signals. As triploid specimens exhibit either total or partial gonad sterility, the present study demonstrates the importance of cytogenetic monitoring in restocking programs aimed at genetic conservation. © 2011 Springer Science+Business Media B.V. 2020-06-15T21:52:18Z 2020-06-15T21:52:18Z 2011 Artigo https://repositorio.inpa.gov.br/handle/1/18174 10.1007/s10641-011-9794-2 en Volume 91, Número 3, Pags. 361-366 Restrito Environmental Biology of Fishes |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Captivity Chromosome Conservation Genetics Cytogenetics Genetic Marker Geographical Distribution Hybridization Molecular Analysis Monitoring System Native Species Ploidy Probe Sterility Teleost Visualization Brasil Iguacu Basin Rhamdia Rhamdia Quelen |
| spellingShingle |
Captivity Chromosome Conservation Genetics Cytogenetics Genetic Marker Geographical Distribution Hybridization Molecular Analysis Monitoring System Native Species Ploidy Probe Sterility Teleost Visualization Brasil Iguacu Basin Rhamdia Rhamdia Quelen Silva, M. R. da Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| topic_facet |
Captivity Chromosome Conservation Genetics Cytogenetics Genetic Marker Geographical Distribution Hybridization Molecular Analysis Monitoring System Native Species Ploidy Probe Sterility Teleost Visualization Brasil Iguacu Basin Rhamdia Rhamdia Quelen |
| description |
The freshwater silver catfish from the genus Rhamdia belongs to a group of neotropical fish with wide distribution throughout Central and South America. The results obtained in the F1 generation of native specimens of this species bred in captivity using molecular cytogenetic tools revealed a high number of triploid specimens (46.8%) in the samples analyzed. The use of double fluorescent in situ hybridization (double-FISH) with 18S and 5S rDNA probes allowed highlighting the location of ribosomal sites with independent activity. This chromosome marker allowed the identification of triploid nuclei, in which three markers were visualized, in contrast to diploid fish, which only exhibited two fluorescent signals. As triploid specimens exhibit either total or partial gonad sterility, the present study demonstrates the importance of cytogenetic monitoring in restocking programs aimed at genetic conservation. © 2011 Springer Science+Business Media B.V. |
| format |
Artigo |
| author |
Silva, M. R. da |
| author2 |
Matoso, D. A. Ludwig, Luis Augusto M. Gomes, Eder Almeida, Mara Cristina de Vicari, Marcelo Ricardo Artoni, Roberto Ferreira |
| author2Str |
Matoso, D. A. Ludwig, Luis Augusto M. Gomes, Eder Almeida, Mara Cristina de Vicari, Marcelo Ricardo Artoni, Roberto Ferreira |
| title |
Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| title_short |
Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| title_full |
Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| title_fullStr |
Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| title_full_unstemmed |
Natural triploidy in Rhamdia quelen identified by cytogenetic monitoring in Iguaçu basin, southern Brazil |
| title_sort |
natural triploidy in rhamdia quelen identified by cytogenetic monitoring in iguaçu basin, southern brazil |
| publisher |
Environmental Biology of Fishes |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18174 |
| _version_ |
1787145276628140032 |
| score |
11.678145 |