Polymorphic microsatellite DNA markers for the Amazonian catfish Pseudoplatystoma punctifer (Siluriformes: Pimelodidae)
The large catfish Pseudoplatystoma punctifer has a high commercial value in the Amazon. Fifteen polymorphic microsatellite loci were isolated and characterized in 36-39 individuals of this species sampled at the Purus river (Amazon Basin). The number of alleles per locus ranged from 2 to 18 (average...
| Autor principal: | Saulo-Machado, Antonio C. |
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| Outros Autores: | Formiga, Kyara M., Ortiz, M. F., Sousa, Adna Cristina Barbosa, Alves-Gomes, José Antônio, Batista, Jacqueline da Silva |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Conservation Genetics Resources
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18204 |
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The large catfish Pseudoplatystoma punctifer has a high commercial value in the Amazon. Fifteen polymorphic microsatellite loci were isolated and characterized in 36-39 individuals of this species sampled at the Purus river (Amazon Basin). The number of alleles per locus ranged from 2 to 18 (average 7.4), the observed heterozygosity (H O) from 0.025 to 0.972 and the expected heterozygosity (H E) from 0.025 to 0.931. Two loci showed a Hardy-Weinberg Equilibrium deviation. No linkage disequilibrium was found among the studied loci. Fourteen microsatellite loci were cross-amplified successfully in six other Pimelodidae species. Therefore, these loci may be valuable for other studies of population genetic structure as well as for catfish conservation in the Amazon basin. © Springer Science+Business Media B.V. 2010. |