Formulation of a liquid medium with wheat bran for the production of laccase by Trametes versicolor in an air-lift bioreactor
The aim of this work was to formulate a liquid medium with wheat bran for the production of Trametes versicolor laccase in an air-lift bioreactor. Univariated experiments were carried out in order to optimize the contents of CuSO4 (0-2 mM), glucose (0-50 g/L), peptone (0-25 g/L) and fermentation par...
| Autor principal: | Souza, João Vicente Braga de |
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| Outros Autores: | Silva, Érica M., Cavallazzi, José Renato Pereira, Sobrinho, Agostinho da S. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Journal of Food, Agriculture and Environment
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18372 |
| Resumo: |
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The aim of this work was to formulate a liquid medium with wheat bran for the production of Trametes versicolor laccase in an air-lift bioreactor. Univariated experiments were carried out in order to optimize the contents of CuSO4 (0-2 mM), glucose (0-50 g/L), peptone (0-25 g/L) and fermentation parameters as pH (3-7), inoculum (40-360 mycelium plugs/L) and aeration (0-1 v/v/m). After the optimization an optimized experiment was accomplished (pH 3, inoculum of 40 mycelium plugs/L, CuSO4 concentration of 1.2 mM, 10 g/L of peptone and the aeration of 0.5 v/v/m) in an airlift reactor. The highest activity (32,000 UI/L) was reached in the 10th experimental day. |