Artigo

Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production

Enokipodins A, B, C, and D are α-cuparene-type sesquiterpenoids antimicrobial metabolites produced in the stationary stage of Flammulina velutipes mycelia development in malt extract broth. This study assessed the influence of nutritional and environmental factors on F. velutipes mycelia culture for...

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Autor principal: Melo, Marcelo Rodrigues de
Outros Autores: Paccola-Meirelles, Luzia Doretto, Faria, Terezinha de Jesus, Ishikawa, Noemia Kazue
Grau: Artigo
Idioma: English
Publicado em: Mycoscience 2020
Assuntos:
Basidiomycota
Flammulina Velutipes
Solanum Tuberosum
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/18475
id oai:repositorio:1-18475
recordtype dspace
spelling oai:repositorio:1-18475 Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production Melo, Marcelo Rodrigues de Paccola-Meirelles, Luzia Doretto Faria, Terezinha de Jesus Ishikawa, Noemia Kazue Basidiomycota Flammulina Velutipes Solanum Tuberosum Enokipodins A, B, C, and D are α-cuparene-type sesquiterpenoids antimicrobial metabolites produced in the stationary stage of Flammulina velutipes mycelia development in malt extract broth. This study assessed the influence of nutritional and environmental factors on F. velutipes mycelia culture for the production of these metabolites. The mycelia growth and antimicrobial activity were assessed by determining dry matter and the diffusion in agar method, respectively. The best F. velutipes mycelia growth was observed in dextrose potato broth, and greater antimicrobial metabolite production occurred in complete Pontecorvo's culture medium. Environmental modifications, such as a rise in temperature from 25° to 37°C on the 15th day of F. velutipes mycelia culture in malt extract and peptone broth, also optimized antimicrobial metabolite production. The metabolites produced in these treatments were correlated with the enokipodins A and B in thin-layer chromatography (TLC) and the antifungal activity test by TLC bioautography. This study showed that there was no correlation between biomass production and antimicrobial metabolite production, but there may be a correlation between culture medium composition and enokipodins biosynthesis. © 2009 The Mycological Society of Japan and Springer. 2020-06-15T21:55:32Z 2020-06-15T21:55:32Z 2009 Artigo https://repositorio.inpa.gov.br/handle/1/18475 10.1007/s10267-008-0447-z en Volume 50, Número 1, Pags. 78-81 Restrito Mycoscience
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
topic Basidiomycota
Flammulina Velutipes
Solanum Tuberosum
spellingShingle Basidiomycota
Flammulina Velutipes
Solanum Tuberosum
Melo, Marcelo Rodrigues de
Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
topic_facet Basidiomycota
Flammulina Velutipes
Solanum Tuberosum
description Enokipodins A, B, C, and D are α-cuparene-type sesquiterpenoids antimicrobial metabolites produced in the stationary stage of Flammulina velutipes mycelia development in malt extract broth. This study assessed the influence of nutritional and environmental factors on F. velutipes mycelia culture for the production of these metabolites. The mycelia growth and antimicrobial activity were assessed by determining dry matter and the diffusion in agar method, respectively. The best F. velutipes mycelia growth was observed in dextrose potato broth, and greater antimicrobial metabolite production occurred in complete Pontecorvo's culture medium. Environmental modifications, such as a rise in temperature from 25° to 37°C on the 15th day of F. velutipes mycelia culture in malt extract and peptone broth, also optimized antimicrobial metabolite production. The metabolites produced in these treatments were correlated with the enokipodins A and B in thin-layer chromatography (TLC) and the antifungal activity test by TLC bioautography. This study showed that there was no correlation between biomass production and antimicrobial metabolite production, but there may be a correlation between culture medium composition and enokipodins biosynthesis. © 2009 The Mycological Society of Japan and Springer.
format Artigo
author Melo, Marcelo Rodrigues de
author2 Paccola-Meirelles, Luzia Doretto
Faria, Terezinha de Jesus
Ishikawa, Noemia Kazue
author2Str Paccola-Meirelles, Luzia Doretto
Faria, Terezinha de Jesus
Ishikawa, Noemia Kazue
title Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
title_short Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
title_full Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
title_fullStr Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
title_full_unstemmed Influence of Flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
title_sort influence of flammulina velutipes mycelia culture conditions on antimicrobial metabolite production
publisher Mycoscience
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/18475
_version_ 1787144139362533376
score 11.675608

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