Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway
Ligands for peroxisome proliferator-activated receptor γ (PPAR-γ), such as 15-deoxy-Δ12,14-prostaglandin J 2 (15d-PGJ2) have been implicated as a new class of anti-inflammatory compounds with possible clinical applications. Based on this concept, this investigation was designed to determine the effe...
| Autor principal: | Napimoga, Marcelo Henrique |
|---|---|
| Outros Autores: | Manfredo Vieira, Silvio, Dal-Secco, Daniela, Freitas, Andressa de, Souto, Fabrício Oliveira, Mestriner, Fabíola Leslie Antunes C., Alves-Filho, J. C., Grespan, Renata, Kawai, Toshihisa, Ferreira, Seérgio Henrique, Cunha, Fernando Queiroz |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Journal of Immunology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18606 |
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oai:repositorio:1-18606 |
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oai:repositorio:1-18606 Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway Napimoga, Marcelo Henrique Manfredo Vieira, Silvio Dal-Secco, Daniela Freitas, Andressa de Souto, Fabrício Oliveira Mestriner, Fabíola Leslie Antunes C. Alves-Filho, J. C. Grespan, Renata Kawai, Toshihisa Ferreira, Seérgio Henrique Cunha, Fernando Queiroz 15 Deoxy Delta12,14 Prostaglandin J2 Carrageenan Chemokine Cytokine F Actin Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Ligand Nitric Oxide Nitric Oxide Synthase Inhibitor Peroxisome Proliferator Activated Receptor Gamma 15 Deoxy Delta12,14 Prostaglandin J2 15 Deoxy Delta12,14 Prostaglandin J2 Actin Carrageenan Drug Derivative Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Nitric Oxide Peroxisome Proliferator Activated Receptor Gamma Prostaglandin D2 Unclassified Drug Animals Cell Animals Experiment Animals Model Animals Tissue Controlled Study Cytokine Production Disease Model Genetic Susceptibility Immunomodulation Immunostimulation Inflammation Leukocyte Adherence Leukocyte Migration Inhibition Leukocyte Rolling Male Mesentery Mesentery Blood Flow Mesentery Blood Vessel Mouse Neutrophil Nonhuman Peritoneal Cavity Peritonitis Priority Journal Protein Expression Up-regulation Wild Type Animals Capillary Cell Adhesion Drug Antagonism Drug Effect Genetics Immunology Leukocyte Rolling Metabolism Microcirculation Mouse Mutant Vascularization Actins Animal Capillaries Carrageenan Cell Adhesion Intercellular Adhesion Molecule-1 Leukocyte Rolling Ligands Male Mesentery Mice Mice, Knockout Microcirculation Neutrophils Nitric Oxide Nitric Oxide Synthase Type Iii Ppar Gamma Prostaglandin D2 Ligands for peroxisome proliferator-activated receptor γ (PPAR-γ), such as 15-deoxy-Δ12,14-prostaglandin J 2 (15d-PGJ2) have been implicated as a new class of anti-inflammatory compounds with possible clinical applications. Based on this concept, this investigation was designed to determine the effect of 15d-PGJ 2-mediated activation of PPAR-γ ligand on neutrophil migration after an inflammatory stimulus and clarify the underlying molecular mechanisms using a mouse model of peritonitis. Our results demonstrated that 15d-PGJ 2 administration decreases leukocyte rolling and adhesion to the inflammated mesenteric tissues by a mechanism dependent on NO. Specifically, pharmacological inhibitors of NO synthase remarkably abrogated the 15d-PGJ 2-mediated suppression of neutrophil migration to the inflammatory site. Moreover, inducible NOS-/- mice were not susceptible to 15d-PGJ2-mediated suppression of neutrophil migration to the inflammatory sites when compared with their wild type. In addition, 15d-PGJ 2-mediated suppression of neutrophil migration appeared to be independent of the production of cytokines and chemokines, since their production were not significantly affected in the carrageenan-injected peritoneal cavities. Finally, upregulation of carrageenan-triggered ICAM-1 expression in the mesenteric microcirculation vessels was abrogated by pretreatment of wild-type mice with 15d-PGJ2, whereas 15d-PGJ 2 inhibited F-actin rearrangement process in neutrophils. Taken together these findings demonstrated that 15d-PGJ2 suppresses inflammation-initiated neutrophil migration in a mechanism dependent on NO production in mesenteric tissues. Copyright © 2007 by The American Association of Immunologists, Inc. 2020-06-15T22:02:15Z 2020-06-15T22:02:15Z 2008 Artigo https://repositorio.inpa.gov.br/handle/1/18606 en Volume 180, Número 1, Pags. 609-617 Restrito Journal of Immunology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
15 Deoxy Delta12,14 Prostaglandin J2 Carrageenan Chemokine Cytokine F Actin Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Ligand Nitric Oxide Nitric Oxide Synthase Inhibitor Peroxisome Proliferator Activated Receptor Gamma 15 Deoxy Delta12,14 Prostaglandin J2 15 Deoxy Delta12,14 Prostaglandin J2 Actin Carrageenan Drug Derivative Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Nitric Oxide Peroxisome Proliferator Activated Receptor Gamma Prostaglandin D2 Unclassified Drug Animals Cell Animals Experiment Animals Model Animals Tissue Controlled Study Cytokine Production Disease Model Genetic Susceptibility Immunomodulation Immunostimulation Inflammation Leukocyte Adherence Leukocyte Migration Inhibition Leukocyte Rolling Male Mesentery Mesentery Blood Flow Mesentery Blood Vessel Mouse Neutrophil Nonhuman Peritoneal Cavity Peritonitis Priority Journal Protein Expression Up-regulation Wild Type Animals Capillary Cell Adhesion Drug Antagonism Drug Effect Genetics Immunology Leukocyte Rolling Metabolism Microcirculation Mouse Mutant Vascularization Actins Animal Capillaries Carrageenan Cell Adhesion Intercellular Adhesion Molecule-1 Leukocyte Rolling Ligands Male Mesentery Mice Mice, Knockout Microcirculation Neutrophils Nitric Oxide Nitric Oxide Synthase Type Iii Ppar Gamma Prostaglandin D2 |
| spellingShingle |
15 Deoxy Delta12,14 Prostaglandin J2 Carrageenan Chemokine Cytokine F Actin Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Ligand Nitric Oxide Nitric Oxide Synthase Inhibitor Peroxisome Proliferator Activated Receptor Gamma 15 Deoxy Delta12,14 Prostaglandin J2 15 Deoxy Delta12,14 Prostaglandin J2 Actin Carrageenan Drug Derivative Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Nitric Oxide Peroxisome Proliferator Activated Receptor Gamma Prostaglandin D2 Unclassified Drug Animals Cell Animals Experiment Animals Model Animals Tissue Controlled Study Cytokine Production Disease Model Genetic Susceptibility Immunomodulation Immunostimulation Inflammation Leukocyte Adherence Leukocyte Migration Inhibition Leukocyte Rolling Male Mesentery Mesentery Blood Flow Mesentery Blood Vessel Mouse Neutrophil Nonhuman Peritoneal Cavity Peritonitis Priority Journal Protein Expression Up-regulation Wild Type Animals Capillary Cell Adhesion Drug Antagonism Drug Effect Genetics Immunology Leukocyte Rolling Metabolism Microcirculation Mouse Mutant Vascularization Actins Animal Capillaries Carrageenan Cell Adhesion Intercellular Adhesion Molecule-1 Leukocyte Rolling Ligands Male Mesentery Mice Mice, Knockout Microcirculation Neutrophils Nitric Oxide Nitric Oxide Synthase Type Iii Ppar Gamma Prostaglandin D2 Napimoga, Marcelo Henrique Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| topic_facet |
15 Deoxy Delta12,14 Prostaglandin J2 Carrageenan Chemokine Cytokine F Actin Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Ligand Nitric Oxide Nitric Oxide Synthase Inhibitor Peroxisome Proliferator Activated Receptor Gamma 15 Deoxy Delta12,14 Prostaglandin J2 15 Deoxy Delta12,14 Prostaglandin J2 Actin Carrageenan Drug Derivative Inducible Nitric Oxide Synthase Intercellular Adhesion Molecule-1 Nitric Oxide Peroxisome Proliferator Activated Receptor Gamma Prostaglandin D2 Unclassified Drug Animals Cell Animals Experiment Animals Model Animals Tissue Controlled Study Cytokine Production Disease Model Genetic Susceptibility Immunomodulation Immunostimulation Inflammation Leukocyte Adherence Leukocyte Migration Inhibition Leukocyte Rolling Male Mesentery Mesentery Blood Flow Mesentery Blood Vessel Mouse Neutrophil Nonhuman Peritoneal Cavity Peritonitis Priority Journal Protein Expression Up-regulation Wild Type Animals Capillary Cell Adhesion Drug Antagonism Drug Effect Genetics Immunology Leukocyte Rolling Metabolism Microcirculation Mouse Mutant Vascularization Actins Animal Capillaries Carrageenan Cell Adhesion Intercellular Adhesion Molecule-1 Leukocyte Rolling Ligands Male Mesentery Mice Mice, Knockout Microcirculation Neutrophils Nitric Oxide Nitric Oxide Synthase Type Iii Ppar Gamma Prostaglandin D2 |
| description |
Ligands for peroxisome proliferator-activated receptor γ (PPAR-γ), such as 15-deoxy-Δ12,14-prostaglandin J 2 (15d-PGJ2) have been implicated as a new class of anti-inflammatory compounds with possible clinical applications. Based on this concept, this investigation was designed to determine the effect of 15d-PGJ 2-mediated activation of PPAR-γ ligand on neutrophil migration after an inflammatory stimulus and clarify the underlying molecular mechanisms using a mouse model of peritonitis. Our results demonstrated that 15d-PGJ 2 administration decreases leukocyte rolling and adhesion to the inflammated mesenteric tissues by a mechanism dependent on NO. Specifically, pharmacological inhibitors of NO synthase remarkably abrogated the 15d-PGJ 2-mediated suppression of neutrophil migration to the inflammatory site. Moreover, inducible NOS-/- mice were not susceptible to 15d-PGJ2-mediated suppression of neutrophil migration to the inflammatory sites when compared with their wild type. In addition, 15d-PGJ 2-mediated suppression of neutrophil migration appeared to be independent of the production of cytokines and chemokines, since their production were not significantly affected in the carrageenan-injected peritoneal cavities. Finally, upregulation of carrageenan-triggered ICAM-1 expression in the mesenteric microcirculation vessels was abrogated by pretreatment of wild-type mice with 15d-PGJ2, whereas 15d-PGJ 2 inhibited F-actin rearrangement process in neutrophils. Taken together these findings demonstrated that 15d-PGJ2 suppresses inflammation-initiated neutrophil migration in a mechanism dependent on NO production in mesenteric tissues. Copyright © 2007 by The American Association of Immunologists, Inc. |
| format |
Artigo |
| author |
Napimoga, Marcelo Henrique |
| author2 |
Manfredo Vieira, Silvio Dal-Secco, Daniela Freitas, Andressa de Souto, Fabrício Oliveira Mestriner, Fabíola Leslie Antunes C. Alves-Filho, J. C. Grespan, Renata Kawai, Toshihisa Ferreira, Seérgio Henrique Cunha, Fernando Queiroz |
| author2Str |
Manfredo Vieira, Silvio Dal-Secco, Daniela Freitas, Andressa de Souto, Fabrício Oliveira Mestriner, Fabíola Leslie Antunes C. Alves-Filho, J. C. Grespan, Renata Kawai, Toshihisa Ferreira, Seérgio Henrique Cunha, Fernando Queiroz |
| title |
Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| title_short |
Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| title_full |
Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| title_fullStr |
Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| title_full_unstemmed |
Peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin J2, reduces neutrophil migration via a nitric oxide pathway |
| title_sort |
peroxisome proliferator-activated receptor-γ ligand, 15-deoxy-Δ12,14-prostaglandin j2, reduces neutrophil migration via a nitric oxide pathway |
| publisher |
Journal of Immunology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18606 |
| _version_ |
1787141937450450944 |
| score |
11.653393 |