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spelling oai:repositorio:1-18656 Cytotoxic fractions from the leaves of Tachia grandiflora Pohlit, Adrian Martin Tigre, Ryuga Frota Coelho Cavalcanti, Bruno Moraes, Manœl Odorico de Costa-Lotufo, Leticia Veras Moraes, Maria Elisabete Amaral de Santos, Elba Vieira Mustafa dos Morais, Sabrina Kelly Reis Nunomura, Sergio Massayoshi Pessoa, Cláudia Ó. Alcohol Butanol Chloroform Doxorubicin Hexane Methanol Plant Extract Silica Gel Tachia Grandiflora Extract Unclassified Drug Water Animals Cell Antineoplastic Activity Brasil Breast Tumor Cancer Inhibition Cell Strain Hl 60 Cell Strain Mcf 7 Colon Tumor Column Chromatography Concentration Response Confidence Interval Controlled Study Cytolysis Drug Activity Drug Cytotoxicity Drug Isolation Drug Research Drug Screening Drug Synthesis Hemolysis Human Human Cell Ic 50 In Vitro Study Leukemia Medicinal Plant Melanoma B16 Mouse Nonhuman Plant Leaf Plant Stem Tachia Grandiflora Cell Line, Tumor Gentianaceae Murinae Tachia Grandiflora This work is part of a larger screening program, which seeks to discover new antitumor plants and compounds from the Brazilian Amazon. In a prescreen of stem and leaf extracts of Tachia grandiflora Maguire & Weaver (Gentianaceae) based on the SRB method, leaf methanol and ethanol extracts showed appreciable cytotoxicity in human breast (MCF-7) and colon (HCT-8) tumor cell lines. Liquid-liquid partitioning of the leaf ethanol extract yielded hexane, chloroform, butanol, and water-methanol fractions. Only the hexane and chloroform fractions were active, inhibiting murine melanoma (B-16) and HCT-8 cells. The chloroform fraction suffered sequential column chromatography on silica gel using different eluent systems and yielded a number of very active subfractions. In all, 25 fractions and subfractions were tested, and 10 exhibited high growth inhibition of HCT-8, and two of these presented strong inhibition of murine melanoma (B-16) cells. The most active subfractions were tested against five tumor cell lines (leukemia CEM and HL-60, as well as the three used previously) using the MTT assay, and four fractions demonstrated significant cytotoxicity based on IC50. Cell lysis was discarded as a possible mechanism for in vitro cytotoxicity given that these fractions did not exhibit hemolytic activity. The greatest antiproliferative potential was found in the second (two samples) and third generation (two samples) chromatographic subfractions of the chloroform fraction (obtained from partitioning of the ethanol extract). These subfractions proved to be complex mixtures from which no pure substance could be isolated after further chromatographic separations. © 2007 Informa Healthcare. 2020-06-15T22:02:27Z 2020-06-15T22:02:27Z 2007 Artigo https://repositorio.inpa.gov.br/handle/1/18656 10.1080/13880200701215422 en Volume 45, Número 5, Pags. 429-433 Restrito Pharmaceutical Biology
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
topic Alcohol
Butanol
Chloroform
Doxorubicin
Hexane
Methanol
Plant Extract
Silica Gel
Tachia Grandiflora Extract
Unclassified Drug
Water
Animals Cell
Antineoplastic Activity
Brasil
Breast Tumor
Cancer Inhibition
Cell Strain Hl 60
Cell Strain Mcf 7
Colon Tumor
Column Chromatography
Concentration Response
Confidence Interval
Controlled Study
Cytolysis
Drug Activity
Drug Cytotoxicity
Drug Isolation
Drug Research
Drug Screening
Drug Synthesis
Hemolysis
Human
Human Cell
Ic 50
In Vitro Study
Leukemia
Medicinal Plant
Melanoma B16
Mouse
Nonhuman
Plant Leaf
Plant Stem
Tachia Grandiflora
Cell Line, Tumor
Gentianaceae
Murinae
Tachia Grandiflora
spellingShingle Alcohol
Butanol
Chloroform
Doxorubicin
Hexane
Methanol
Plant Extract
Silica Gel
Tachia Grandiflora Extract
Unclassified Drug
Water
Animals Cell
Antineoplastic Activity
Brasil
Breast Tumor
Cancer Inhibition
Cell Strain Hl 60
Cell Strain Mcf 7
Colon Tumor
Column Chromatography
Concentration Response
Confidence Interval
Controlled Study
Cytolysis
Drug Activity
Drug Cytotoxicity
Drug Isolation
Drug Research
Drug Screening
Drug Synthesis
Hemolysis
Human
Human Cell
Ic 50
In Vitro Study
Leukemia
Medicinal Plant
Melanoma B16
Mouse
Nonhuman
Plant Leaf
Plant Stem
Tachia Grandiflora
Cell Line, Tumor
Gentianaceae
Murinae
Tachia Grandiflora
Pohlit, Adrian Martin
Cytotoxic fractions from the leaves of Tachia grandiflora
topic_facet Alcohol
Butanol
Chloroform
Doxorubicin
Hexane
Methanol
Plant Extract
Silica Gel
Tachia Grandiflora Extract
Unclassified Drug
Water
Animals Cell
Antineoplastic Activity
Brasil
Breast Tumor
Cancer Inhibition
Cell Strain Hl 60
Cell Strain Mcf 7
Colon Tumor
Column Chromatography
Concentration Response
Confidence Interval
Controlled Study
Cytolysis
Drug Activity
Drug Cytotoxicity
Drug Isolation
Drug Research
Drug Screening
Drug Synthesis
Hemolysis
Human
Human Cell
Ic 50
In Vitro Study
Leukemia
Medicinal Plant
Melanoma B16
Mouse
Nonhuman
Plant Leaf
Plant Stem
Tachia Grandiflora
Cell Line, Tumor
Gentianaceae
Murinae
Tachia Grandiflora
description This work is part of a larger screening program, which seeks to discover new antitumor plants and compounds from the Brazilian Amazon. In a prescreen of stem and leaf extracts of Tachia grandiflora Maguire & Weaver (Gentianaceae) based on the SRB method, leaf methanol and ethanol extracts showed appreciable cytotoxicity in human breast (MCF-7) and colon (HCT-8) tumor cell lines. Liquid-liquid partitioning of the leaf ethanol extract yielded hexane, chloroform, butanol, and water-methanol fractions. Only the hexane and chloroform fractions were active, inhibiting murine melanoma (B-16) and HCT-8 cells. The chloroform fraction suffered sequential column chromatography on silica gel using different eluent systems and yielded a number of very active subfractions. In all, 25 fractions and subfractions were tested, and 10 exhibited high growth inhibition of HCT-8, and two of these presented strong inhibition of murine melanoma (B-16) cells. The most active subfractions were tested against five tumor cell lines (leukemia CEM and HL-60, as well as the three used previously) using the MTT assay, and four fractions demonstrated significant cytotoxicity based on IC50. Cell lysis was discarded as a possible mechanism for in vitro cytotoxicity given that these fractions did not exhibit hemolytic activity. The greatest antiproliferative potential was found in the second (two samples) and third generation (two samples) chromatographic subfractions of the chloroform fraction (obtained from partitioning of the ethanol extract). These subfractions proved to be complex mixtures from which no pure substance could be isolated after further chromatographic separations. © 2007 Informa Healthcare.
format Artigo
author Pohlit, Adrian Martin
author2 Tigre, Ryuga Frota
Coelho Cavalcanti, Bruno
Moraes, Manœl Odorico de
Costa-Lotufo, Leticia Veras
Moraes, Maria Elisabete Amaral de
Santos, Elba Vieira Mustafa dos
Morais, Sabrina Kelly Reis
Nunomura, Sergio Massayoshi
Pessoa, Cláudia Ó.
author2Str Tigre, Ryuga Frota
Coelho Cavalcanti, Bruno
Moraes, Manœl Odorico de
Costa-Lotufo, Leticia Veras
Moraes, Maria Elisabete Amaral de
Santos, Elba Vieira Mustafa dos
Morais, Sabrina Kelly Reis
Nunomura, Sergio Massayoshi
Pessoa, Cláudia Ó.
title Cytotoxic fractions from the leaves of Tachia grandiflora
title_short Cytotoxic fractions from the leaves of Tachia grandiflora
title_full Cytotoxic fractions from the leaves of Tachia grandiflora
title_fullStr Cytotoxic fractions from the leaves of Tachia grandiflora
title_full_unstemmed Cytotoxic fractions from the leaves of Tachia grandiflora
title_sort cytotoxic fractions from the leaves of tachia grandiflora
publisher Pharmaceutical Biology
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/18656
_version_ 1787143195859091456
score 11.755432

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