Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences
Species identification of anopheline mosquitoes (Diptera: Culicidae) can be problematic because many of them belong to complexes of morphologically similar species, often with contrasted ecology, behaviour and vectorial importance. The application of DNA-based diagnostics has proved to be useful for...
| Autor principal: | Marrelli, Mauro Toledo |
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| Outros Autores: | Flöeter-Winter, Lucile Maria F., Malafronte, Rosely dos Santos, Tadei, Wanderli Pedro, Lourenço-De-Oliveira, Ricardo, Flores-Mendoza, Carmen, Marinotti, Osvaldo |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Medical and Veterinary Entomology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18840 |
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oai:repositorio:1-18840 |
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oai:repositorio:1-18840 Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences Marrelli, Mauro Toledo Flöeter-Winter, Lucile Maria F. Malafronte, Rosely dos Santos Tadei, Wanderli Pedro Lourenço-De-Oliveira, Ricardo Flores-Mendoza, Carmen Marinotti, Osvaldo Dna, Ribosomal Spacer Disease Vector Animals Anopheles Classification Disease Carrier Disease Transmission Genetics Malaria Molecular Genetics Nucleotide Sequence Phylogeny Sequence Alignment Sequence Homology Animal Anopheles Base Sequence Dna, Ribosomal Spacer Insect Vectors Malaria Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Nucleic Acid Albitarsis Culicidae Diptera Nyssorhynchus Oswaldoi Species identification of anopheline mosquitoes (Diptera: Culicidae) can be problematic because many of them belong to complexes of morphologically similar species, often with contrasted ecology, behaviour and vectorial importance. The application of DNA-based diagnostics has proved to be useful for distinguishing between such species. We determined ribosomal DNA sequences of the second internal transcribed spacer (ITS2) from samples of 16 species of Anopheles captured in the Amazon Basin, Brazil. Length of the ITS2 varied from 323 to 410 base pairs, with GC content ranging from 50.7% to 66.5% and sequence identity from 25% to 99% between species. Maximum-likelihood PAUP analysis separated two distinct groups of species conforming with the recognized subgenera Anopheles (represented by eiseni, mattogrossensis, mediopunctatus and peryassui) and Nyssorhynchus (represented by 12 spp.). For the latter group, the neighbour-joining tree generated from rDNA sequence ITS2 relationships is compatible with the morphological taxonomic key established for these Amazonian species: albitarsis, aquasalis, benarrochi, braziliensis, darlingi, deaneorum, dunhami, evansae, nuneztovari, oswaldoi, rangeli and triannulatus. These ITS2 sequence data proved to be a useful tool for species identification and, potentially, to solve taxonomic problems. © 2005 The Royal Entomological Society. 2020-06-15T22:03:22Z 2020-06-15T22:03:22Z 2005 Artigo https://repositorio.inpa.gov.br/handle/1/18840 10.1111/j.0269-283X.2005.00558.x en Volume 19, Número 2, Pags. 208-218 Restrito Medical and Veterinary Entomology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Dna, Ribosomal Spacer Disease Vector Animals Anopheles Classification Disease Carrier Disease Transmission Genetics Malaria Molecular Genetics Nucleotide Sequence Phylogeny Sequence Alignment Sequence Homology Animal Anopheles Base Sequence Dna, Ribosomal Spacer Insect Vectors Malaria Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Nucleic Acid Albitarsis Culicidae Diptera Nyssorhynchus Oswaldoi |
| spellingShingle |
Dna, Ribosomal Spacer Disease Vector Animals Anopheles Classification Disease Carrier Disease Transmission Genetics Malaria Molecular Genetics Nucleotide Sequence Phylogeny Sequence Alignment Sequence Homology Animal Anopheles Base Sequence Dna, Ribosomal Spacer Insect Vectors Malaria Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Nucleic Acid Albitarsis Culicidae Diptera Nyssorhynchus Oswaldoi Marrelli, Mauro Toledo Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| topic_facet |
Dna, Ribosomal Spacer Disease Vector Animals Anopheles Classification Disease Carrier Disease Transmission Genetics Malaria Molecular Genetics Nucleotide Sequence Phylogeny Sequence Alignment Sequence Homology Animal Anopheles Base Sequence Dna, Ribosomal Spacer Insect Vectors Malaria Molecular Sequence Data Phylogeny Sequence Alignment Sequence Homology, Nucleic Acid Albitarsis Culicidae Diptera Nyssorhynchus Oswaldoi |
| description |
Species identification of anopheline mosquitoes (Diptera: Culicidae) can be problematic because many of them belong to complexes of morphologically similar species, often with contrasted ecology, behaviour and vectorial importance. The application of DNA-based diagnostics has proved to be useful for distinguishing between such species. We determined ribosomal DNA sequences of the second internal transcribed spacer (ITS2) from samples of 16 species of Anopheles captured in the Amazon Basin, Brazil. Length of the ITS2 varied from 323 to 410 base pairs, with GC content ranging from 50.7% to 66.5% and sequence identity from 25% to 99% between species. Maximum-likelihood PAUP analysis separated two distinct groups of species conforming with the recognized subgenera Anopheles (represented by eiseni, mattogrossensis, mediopunctatus and peryassui) and Nyssorhynchus (represented by 12 spp.). For the latter group, the neighbour-joining tree generated from rDNA sequence ITS2 relationships is compatible with the morphological taxonomic key established for these Amazonian species: albitarsis, aquasalis, benarrochi, braziliensis, darlingi, deaneorum, dunhami, evansae, nuneztovari, oswaldoi, rangeli and triannulatus. These ITS2 sequence data proved to be a useful tool for species identification and, potentially, to solve taxonomic problems. © 2005 The Royal Entomological Society. |
| format |
Artigo |
| author |
Marrelli, Mauro Toledo |
| author2 |
Flöeter-Winter, Lucile Maria F. Malafronte, Rosely dos Santos Tadei, Wanderli Pedro Lourenço-De-Oliveira, Ricardo Flores-Mendoza, Carmen Marinotti, Osvaldo |
| author2Str |
Flöeter-Winter, Lucile Maria F. Malafronte, Rosely dos Santos Tadei, Wanderli Pedro Lourenço-De-Oliveira, Ricardo Flores-Mendoza, Carmen Marinotti, Osvaldo |
| title |
Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| title_short |
Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| title_full |
Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| title_fullStr |
Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| title_full_unstemmed |
Amazonian malaria vector anopheline relationships interpreted from ITS2 rDNA sequences |
| title_sort |
amazonian malaria vector anopheline relationships interpreted from its2 rdna sequences |
| publisher |
Medical and Veterinary Entomology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18840 |
| _version_ |
1787141787568046080 |
| score |
11.755432 |