Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil
Isozyme electrophoresis studies were conduced on Anopheles oswaldoi sensu lato from three states in Amazonian (Brazil), including Acre, where this taxon has been incriminated as a potential human malaria vector. The 13 enzymes analyzed yielded a total of 20 loci. Of these, 10 were monomorphic in the...
| Autor principal: | Scarpassa, Vera Margarete |
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| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Journal of Medical Entomology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18843 |
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oai:repositorio:1-18843 |
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oai:repositorio:1-18843 Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil Scarpassa, Vera Margarete Isoenzyme Allele Animals Anopheles Brasil Disease Carrier Disease Transmission Enzymology Gene Frequency Polymorphism, Genetic Genetics Heterozygote Human Malaria Alleles Animal Anopheles Brasil Gene Frequency Heterozygote Humans Insect Vectors Isoenzymes Malaria Polymorphism, Genetic Anopheles Konderi Anopheles Oswaldoi Culicidae Diptera Oswaldoi Rondonia Isozyme electrophoresis studies were conduced on Anopheles oswaldoi sensu lato from three states in Amazonian (Brazil), including Acre, where this taxon has been incriminated as a potential human malaria vector. The 13 enzymes analyzed yielded a total of 20 loci. Of these, 10 were monomorphic in the three samples. Diagnostic loci were not found. The measures of genetic variability showed mean number of alleles per locus between 1.5 and 2.0, percentage of polymorphic loci of 40%, and mean heterozygosities from 0.031 to 0.062. The inbreeding coefficient FIS had a moderate mean value (0.109), which resulted from heterozygote genotype deficiencies and the presence of rare alleles in the homozygote state. Mean FST value (0.0502) and genetic distance values (Nei's genetic similarity values of 0.994-0.997; Roger's genetic similarity values of 0.963-0.968) were very low among the three samples of An. oswaldoi s. l. Preliminary results of identifications of male genitalia indicated that An. oswaldoi s. s. Peryassú and Anopheles konderi Galvão & Damasceno of the An. oswaldoi complex coexist sympatrically in Sena Madureira, Acre, and São Miguel, Rondônia, whereas in the sample from Coari, Amazonas, only An. konderi was identified. The isozyme results do not suggest differences between these species, particularly in areas of sympatry. © 2005 Entomological Society of America. 2020-06-15T22:03:23Z 2020-06-15T22:03:23Z 2005 Artigo https://repositorio.inpa.gov.br/handle/1/18843 en Volume 42, Número 3, Pags. 319-326 Restrito Journal of Medical Entomology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Isoenzyme Allele Animals Anopheles Brasil Disease Carrier Disease Transmission Enzymology Gene Frequency Polymorphism, Genetic Genetics Heterozygote Human Malaria Alleles Animal Anopheles Brasil Gene Frequency Heterozygote Humans Insect Vectors Isoenzymes Malaria Polymorphism, Genetic Anopheles Konderi Anopheles Oswaldoi Culicidae Diptera Oswaldoi Rondonia |
| spellingShingle |
Isoenzyme Allele Animals Anopheles Brasil Disease Carrier Disease Transmission Enzymology Gene Frequency Polymorphism, Genetic Genetics Heterozygote Human Malaria Alleles Animal Anopheles Brasil Gene Frequency Heterozygote Humans Insect Vectors Isoenzymes Malaria Polymorphism, Genetic Anopheles Konderi Anopheles Oswaldoi Culicidae Diptera Oswaldoi Rondonia Scarpassa, Vera Margarete Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| topic_facet |
Isoenzyme Allele Animals Anopheles Brasil Disease Carrier Disease Transmission Enzymology Gene Frequency Polymorphism, Genetic Genetics Heterozygote Human Malaria Alleles Animal Anopheles Brasil Gene Frequency Heterozygote Humans Insect Vectors Isoenzymes Malaria Polymorphism, Genetic Anopheles Konderi Anopheles Oswaldoi Culicidae Diptera Oswaldoi Rondonia |
| description |
Isozyme electrophoresis studies were conduced on Anopheles oswaldoi sensu lato from three states in Amazonian (Brazil), including Acre, where this taxon has been incriminated as a potential human malaria vector. The 13 enzymes analyzed yielded a total of 20 loci. Of these, 10 were monomorphic in the three samples. Diagnostic loci were not found. The measures of genetic variability showed mean number of alleles per locus between 1.5 and 2.0, percentage of polymorphic loci of 40%, and mean heterozygosities from 0.031 to 0.062. The inbreeding coefficient FIS had a moderate mean value (0.109), which resulted from heterozygote genotype deficiencies and the presence of rare alleles in the homozygote state. Mean FST value (0.0502) and genetic distance values (Nei's genetic similarity values of 0.994-0.997; Roger's genetic similarity values of 0.963-0.968) were very low among the three samples of An. oswaldoi s. l. Preliminary results of identifications of male genitalia indicated that An. oswaldoi s. s. Peryassú and Anopheles konderi Galvão & Damasceno of the An. oswaldoi complex coexist sympatrically in Sena Madureira, Acre, and São Miguel, Rondônia, whereas in the sample from Coari, Amazonas, only An. konderi was identified. The isozyme results do not suggest differences between these species, particularly in areas of sympatry. © 2005 Entomological Society of America. |
| format |
Artigo |
| author |
Scarpassa, Vera Margarete |
| title |
Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| title_short |
Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| title_full |
Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| title_fullStr |
Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| title_full_unstemmed |
Isozyme similarity in Anopheles oswaldoi sensu lato (Diptera: Culicidae) from the Amazon Region, Brazil |
| title_sort |
isozyme similarity in anopheles oswaldoi sensu lato (diptera: culicidae) from the amazon region, brazil |
| publisher |
Journal of Medical Entomology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18843 |
| _version_ |
1787142113543061504 |
| score |
11.755432 |