Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia
The effects of graded hypoxia on the physiological and biochemical responses were examined in two closely related species of cichlids of the Amazon: Astronotus crassipinnis and Symphysodon aequifasciatus. Ten fish of each species were exposed to graded hypoxia for 8 h in seven oxygen concentrations...
| Autor principal: | Chippari-Gomes, Adriana Regina |
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| Outros Autores: | Gomes, Levy de Carvalho, Lopes, Nilva Pereira, Val, Adalberto Luis, Almeida-Val, Vera Maria Fonseca |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18862 |
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oai:repositorio:1-18862 |
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oai:repositorio:1-18862 Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia Chippari-Gomes, Adriana Regina Gomes, Levy de Carvalho Lopes, Nilva Pereira Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca Citrate Synthase Glycogen Lactate Dehydrogenase Malate Dehydrogenase Oxygen Pyruvate Kinase Anaerobic Metabolism Animals Experiment Animals Tissue Anoxia Biochemistry Brasil Cichlid Comparative Study Concentration (parameters) Controlled Study Down Regulation Enzyme Activity Exposure Glucose Blood Level Glycogen Analysis Heart Muscle Hypoxia Lactate Blood Level Liver Muscle Tissue Nonhuman Oxygen Consumption Physiology Priority Journal Muscle, Skeletal Tissue Level Animalsia Astronotus Crassipinnis Cichlidae Symphysodon Aequifasciatus The effects of graded hypoxia on the physiological and biochemical responses were examined in two closely related species of cichlids of the Amazon: Astronotus crassipinnis and Symphysodon aequifasciatus. Ten fish of each species were exposed to graded hypoxia for 8 h in seven oxygen concentrations (5.92, 3.15, 1.54, 0.79, 0.60, 0.34, and 0.06 mg O2 L-1), with the aim to evaluate hypoxia tolerance and metabolic adjustments, where plasma glucose and lactate levels, hepatic and muscle glycogen contents, and maximum enzyme activities (PK, LDH, MDH and CS) in skeletal and cardiac muscles were measured. Another experimental set was done to quantify oxygen consumption (MO2) and opercular movements in two oxygen concentrations. Hypoxia tolerance differed between the two species. Astronotus crassipinnis was able to tolerate anoxia for 178 min while S. aequifasciatus was able to withstand 222 min exposure in deep hypoxia (0.75 mg O2 L-1). Suppressed MO2 was observed during exposure to 0.34 (A. crassipinnis) and 0.79 mg O2 L-1 (S. aequifasciatus), while opercular movements increased in both species exposed to hypoxia. Higher levels of muscle and liver glycogen and larger hypoxia-induced increases in plasma glucose and lactate were observed in A. crassipinnis, which showed a higher degree of hypoxia tolerance. Changes in enzyme levels were tissue-specific and differed between species suggesting differential abilities in down-regulating oxidative pathways and increasing anaerobic metabolism. Based on the present data, we conclude that these animals are good anaerobes and highly adapted to their environment, which is allowed by their abilities to regulate metabolic pathways and adjust their enzyme levels. © 2005 Elsevier Inc. All rights reserved. 2020-06-15T22:03:32Z 2020-06-15T22:03:32Z 2005 Artigo https://repositorio.inpa.gov.br/handle/1/18862 10.1016/j.cbpc.2005.04.006 en Volume 141, Número 3, Pags. 347-355 Restrito Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Citrate Synthase Glycogen Lactate Dehydrogenase Malate Dehydrogenase Oxygen Pyruvate Kinase Anaerobic Metabolism Animals Experiment Animals Tissue Anoxia Biochemistry Brasil Cichlid Comparative Study Concentration (parameters) Controlled Study Down Regulation Enzyme Activity Exposure Glucose Blood Level Glycogen Analysis Heart Muscle Hypoxia Lactate Blood Level Liver Muscle Tissue Nonhuman Oxygen Consumption Physiology Priority Journal Muscle, Skeletal Tissue Level Animalsia Astronotus Crassipinnis Cichlidae Symphysodon Aequifasciatus |
| spellingShingle |
Citrate Synthase Glycogen Lactate Dehydrogenase Malate Dehydrogenase Oxygen Pyruvate Kinase Anaerobic Metabolism Animals Experiment Animals Tissue Anoxia Biochemistry Brasil Cichlid Comparative Study Concentration (parameters) Controlled Study Down Regulation Enzyme Activity Exposure Glucose Blood Level Glycogen Analysis Heart Muscle Hypoxia Lactate Blood Level Liver Muscle Tissue Nonhuman Oxygen Consumption Physiology Priority Journal Muscle, Skeletal Tissue Level Animalsia Astronotus Crassipinnis Cichlidae Symphysodon Aequifasciatus Chippari-Gomes, Adriana Regina Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| topic_facet |
Citrate Synthase Glycogen Lactate Dehydrogenase Malate Dehydrogenase Oxygen Pyruvate Kinase Anaerobic Metabolism Animals Experiment Animals Tissue Anoxia Biochemistry Brasil Cichlid Comparative Study Concentration (parameters) Controlled Study Down Regulation Enzyme Activity Exposure Glucose Blood Level Glycogen Analysis Heart Muscle Hypoxia Lactate Blood Level Liver Muscle Tissue Nonhuman Oxygen Consumption Physiology Priority Journal Muscle, Skeletal Tissue Level Animalsia Astronotus Crassipinnis Cichlidae Symphysodon Aequifasciatus |
| description |
The effects of graded hypoxia on the physiological and biochemical responses were examined in two closely related species of cichlids of the Amazon: Astronotus crassipinnis and Symphysodon aequifasciatus. Ten fish of each species were exposed to graded hypoxia for 8 h in seven oxygen concentrations (5.92, 3.15, 1.54, 0.79, 0.60, 0.34, and 0.06 mg O2 L-1), with the aim to evaluate hypoxia tolerance and metabolic adjustments, where plasma glucose and lactate levels, hepatic and muscle glycogen contents, and maximum enzyme activities (PK, LDH, MDH and CS) in skeletal and cardiac muscles were measured. Another experimental set was done to quantify oxygen consumption (MO2) and opercular movements in two oxygen concentrations. Hypoxia tolerance differed between the two species. Astronotus crassipinnis was able to tolerate anoxia for 178 min while S. aequifasciatus was able to withstand 222 min exposure in deep hypoxia (0.75 mg O2 L-1). Suppressed MO2 was observed during exposure to 0.34 (A. crassipinnis) and 0.79 mg O2 L-1 (S. aequifasciatus), while opercular movements increased in both species exposed to hypoxia. Higher levels of muscle and liver glycogen and larger hypoxia-induced increases in plasma glucose and lactate were observed in A. crassipinnis, which showed a higher degree of hypoxia tolerance. Changes in enzyme levels were tissue-specific and differed between species suggesting differential abilities in down-regulating oxidative pathways and increasing anaerobic metabolism. Based on the present data, we conclude that these animals are good anaerobes and highly adapted to their environment, which is allowed by their abilities to regulate metabolic pathways and adjust their enzyme levels. © 2005 Elsevier Inc. All rights reserved. |
| format |
Artigo |
| author |
Chippari-Gomes, Adriana Regina |
| author2 |
Gomes, Levy de Carvalho Lopes, Nilva Pereira Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca |
| author2Str |
Gomes, Levy de Carvalho Lopes, Nilva Pereira Val, Adalberto Luis Almeida-Val, Vera Maria Fonseca |
| title |
Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| title_short |
Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| title_full |
Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| title_fullStr |
Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| title_full_unstemmed |
Metabolic adjustments in two Amazonian cichlids exposed to hypoxia and anoxia |
| title_sort |
metabolic adjustments in two amazonian cichlids exposed to hypoxia and anoxia |
| publisher |
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/18862 |
| _version_ |
1787141495360323584 |
| score |
11.653393 |