Seed protein variation among pepper (Capsicum sp.) genotypes revealed by Maldi-TOF Analysis
A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic simil...
| Autor principal: | Pessoa-Filho, Marco Aurélio Caldas de Pinho |
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| Outros Autores: | Bloch, Carlos Junior, Silva Filho, Danilo Fernandes da, Sobreira Galdino, Alexsandro, Cunha, Rodrigo Maranguape Silva da, Oliveira Alves, Maria Aparecida, Grangeiro, Thalles Barbosa |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Protein and Peptide Letters
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/18936 |
| Resumo: |
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A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccard's coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases. |