Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species
Mahogany (Swietenia macrophylla King [Meliaceae]) is the most valuable hardwood species in the neotropics. Its conservation status has been the subject of increasing concern due to overexploitation and habitat destruction. In this work we report the development and characterization of 10 highly vari...
| Autor principal: | Lemes, Maristerra R. |
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| Outros Autores: | Brondani, Rosana Pereira Vianello, Grattapaglia, Dario |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Journal of Heredity
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19055 |
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oai:repositorio:1-19055 |
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oai:repositorio:1-19055 Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species Lemes, Maristerra R. Brondani, Rosana Pereira Vianello Grattapaglia, Dario Conservation Genetics Gene Flow Gene Locus Gene Sequence Genetic Analysis Genotype Heterozygosity Inheritance Mating System Microsatellite Marker Cloning, Molecular Neotropics Nonhuman Paternity Plant Genetics Pollination Polymerase Chain Reaction Genetics, Population Priority Journal Probability Species Tree Dna Fluorescent Dyes Genetic Markers Meliaceae Microsatellite Repeats Sequence Analysis, Dna Embryophyta Meliaceae Swietenia Swietenia Macrophylla Mahogany (Swietenia macrophylla King [Meliaceae]) is the most valuable hardwood species in the neotropics. Its conservation status has been the subject of increasing concern due to overexploitation and habitat destruction. In this work we report the development and characterization of 10 highly variable microsatellite loci for S. macrophylla. Twenty-nine percent of the 126 sequenced mahogany clones yielded useful microsatellite loci. Three highthroughput genotyping systems were developed based on polymerase chain reaction (PCR) multiplexing of these mahogany loci. We identified a total of 158 alleles in 121 adult individuals of S. macrophylla, with an average of 15.8 alleles (range 11-25) per locus. All loci showed Mendelian inheritance in open-pollinated half-sib families. The mean expected heterozygosity was 0.84 and the mean observed heterozygosity was 0.73. The combined probability of identity-the probability that two individuals selected at random from a population would have identica genotypes-was 7.0 × 10-15, and combined probability of paternity exclusion was 0.999998 overall loci. These microsatellite loci permit precise estimates of parameters such as gene flow, mating system, and paternity, thus providing important insights into the population genetics and conservation of S. macrophylla. 2020-06-15T22:04:59Z 2020-06-15T22:04:59Z 2002 Artigo https://repositorio.inpa.gov.br/handle/1/19055 en Volume 93, Número 4, Pags. 287-291 Restrito Journal of Heredity |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Conservation Genetics Gene Flow Gene Locus Gene Sequence Genetic Analysis Genotype Heterozygosity Inheritance Mating System Microsatellite Marker Cloning, Molecular Neotropics Nonhuman Paternity Plant Genetics Pollination Polymerase Chain Reaction Genetics, Population Priority Journal Probability Species Tree Dna Fluorescent Dyes Genetic Markers Meliaceae Microsatellite Repeats Sequence Analysis, Dna Embryophyta Meliaceae Swietenia Swietenia Macrophylla |
| spellingShingle |
Conservation Genetics Gene Flow Gene Locus Gene Sequence Genetic Analysis Genotype Heterozygosity Inheritance Mating System Microsatellite Marker Cloning, Molecular Neotropics Nonhuman Paternity Plant Genetics Pollination Polymerase Chain Reaction Genetics, Population Priority Journal Probability Species Tree Dna Fluorescent Dyes Genetic Markers Meliaceae Microsatellite Repeats Sequence Analysis, Dna Embryophyta Meliaceae Swietenia Swietenia Macrophylla Lemes, Maristerra R. Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| topic_facet |
Conservation Genetics Gene Flow Gene Locus Gene Sequence Genetic Analysis Genotype Heterozygosity Inheritance Mating System Microsatellite Marker Cloning, Molecular Neotropics Nonhuman Paternity Plant Genetics Pollination Polymerase Chain Reaction Genetics, Population Priority Journal Probability Species Tree Dna Fluorescent Dyes Genetic Markers Meliaceae Microsatellite Repeats Sequence Analysis, Dna Embryophyta Meliaceae Swietenia Swietenia Macrophylla |
| description |
Mahogany (Swietenia macrophylla King [Meliaceae]) is the most valuable hardwood species in the neotropics. Its conservation status has been the subject of increasing concern due to overexploitation and habitat destruction. In this work we report the development and characterization of 10 highly variable microsatellite loci for S. macrophylla. Twenty-nine percent of the 126 sequenced mahogany clones yielded useful microsatellite loci. Three highthroughput genotyping systems were developed based on polymerase chain reaction (PCR) multiplexing of these mahogany loci. We identified a total of 158 alleles in 121 adult individuals of S. macrophylla, with an average of 15.8 alleles (range 11-25) per locus. All loci showed Mendelian inheritance in open-pollinated half-sib families. The mean expected heterozygosity was 0.84 and the mean observed heterozygosity was 0.73. The combined probability of identity-the probability that two individuals selected at random from a population would have identica genotypes-was 7.0 × 10-15, and combined probability of paternity exclusion was 0.999998 overall loci. These microsatellite loci permit precise estimates of parameters such as gene flow, mating system, and paternity, thus providing important insights into the population genetics and conservation of S. macrophylla. |
| format |
Artigo |
| author |
Lemes, Maristerra R. |
| author2 |
Brondani, Rosana Pereira Vianello Grattapaglia, Dario |
| author2Str |
Brondani, Rosana Pereira Vianello Grattapaglia, Dario |
| title |
Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| title_short |
Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| title_full |
Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| title_fullStr |
Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| title_full_unstemmed |
Multiplexed systems of microsatellite markers for genetic analysis of mahogany, Swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| title_sort |
multiplexed systems of microsatellite markers for genetic analysis of mahogany, swietenia macrophylla king (meliaceae), a threatened neotropical timber species |
| publisher |
Journal of Heredity |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19055 |
| _version_ |
1787141789595992064 |
| score |
11.755432 |