Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)

Brauner, Colin John; Wang, Tobias; Val, Adalberto Luis; Jensen, Frank Bo

Artigo

Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)

Some teleost fishes exhibit a non-linear release of H+ with haemoglobin oxygen saturation (SO2) in whole blood that may be related to the Root effect and a low apparent cooperativity in oxygen binding. To further investigate this correlation, the relationship between red cell pH (pHi) and SO2 was ev...

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Autor principal:	Brauner, Colin John
Outros Autores:	Wang, Tobias, Val, Adalberto Luis, Jensen, Frank Bo
Grau:	Artigo
Idioma:	English
Publicado em:	Fish Physiology and Biochemistry 2020
Assuntos:	Carbon Dioxide Hemoglobin Proton Acid Base Balance Acidity Animals Cell Animals Experiment Blood Oxygenation Bohr Effect Carbon Dioxide Blood Level Carp Cell Ph Cell Suspension Constants And Coefficients Controlled Study Erythrocyte Evaluation Gas Transport Hematological Parameters In Vivo Study Nonhuman Nonlinear System Oxygen Saturation Oxygen Tension Proton Transport Species Difference Teleost Animalsia Colossoma Macropomum Colossoma Marcopomum Cyprinus Carpio Pisces Teleostei
Acesso em linha:	https://repositorio.inpa.gov.br/handle/1/19104

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recordtype	dspace
spelling	oai:repositorio:1-19104 Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum) Brauner, Colin John Wang, Tobias Val, Adalberto Luis Jensen, Frank Bo Carbon Dioxide Hemoglobin Proton Acid Base Balance Acidity Animals Cell Animals Experiment Blood Oxygenation Bohr Effect Carbon Dioxide Blood Level Carp Cell Ph Cell Suspension Constants And Coefficients Controlled Study Erythrocyte Evaluation Gas Transport Hematological Parameters In Vivo Study Nonhuman Nonlinear System Oxygen Saturation Oxygen Tension Proton Transport Species Difference Teleost Animalsia Colossoma Macropomum Colossoma Marcopomum Cyprinus Carpio Pisces Teleostei Some teleost fishes exhibit a non-linear release of H+ with haemoglobin oxygen saturation (SO2) in whole blood that may be related to the Root effect and a low apparent cooperativity in oxygen binding. To further investigate this correlation, the relationship between red cell pH (pHi) and SO2 was evaluated in two teleost fishes, the carp (Cyprinus carpio) and tambaqui (Colossoma macropomum). Carp possess a relatively small Root effect within the physiological pH range, while that in tambaqui is relatively large. It was therefore hypothesized that both species would possess a non-linear release of H+ with SO2 under in vivo conditions, but that the degree of non-linearity would be less pronounced in carp than in tambaqui. Red cell suspensions of tambaqui showed a marked non-linear relationship between pHi and SO2 at in vivo pH values (pHe = 7.66), where the majority of Bohr protons were released between 50 and 100% SO2. In whole blood of carp, the relationship between pHi and SO2 was almost linear at normal resting extracellular pH values (pHe = 8.10) where Hills n50 (cooperativity) was 1.3. Under acidic conditions (pHe = 7.11), n50 decreased to 0.9 and the release of H+ with SO2 became slightly non-linear. The reduction in pH in carp blood was associated with the onset of the Root effect, and the oxygen tension at half saturation (P50) increased from 3.8 to 38.2 mmHg. The relationship between total CO2 and SO2 in carp whole blood changed from being almost linear at pHe 8.1 to being non-linear at pHe 7.1, consistent with the relationship between pHi and SO2. Thus, possession of a Root effect could be a prerequisite for a non-linear release of Bohr protons with oxygenation, but the expression in whole blood may depend upon a species-specific cooperativity threshold. The non-linear release of Bohr protons with oxygenation may be a general phenomenon in teleosts with important implications for gas transport and acid-base homeostasis. 2020-06-15T22:05:24Z 2020-06-15T22:05:24Z 2001 Artigo https://repositorio.inpa.gov.br/handle/1/19104 10.1023/A:1011944407938 en Volume 24, Número 2, Pags. 97-104 Restrito Fish Physiology and Biochemistry
institution	Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection	INPA-RI
language	English
topic	Carbon Dioxide Hemoglobin Proton Acid Base Balance Acidity Animals Cell Animals Experiment Blood Oxygenation Bohr Effect Carbon Dioxide Blood Level Carp Cell Ph Cell Suspension Constants And Coefficients Controlled Study Erythrocyte Evaluation Gas Transport Hematological Parameters In Vivo Study Nonhuman Nonlinear System Oxygen Saturation Oxygen Tension Proton Transport Species Difference Teleost Animalsia Colossoma Macropomum Colossoma Marcopomum Cyprinus Carpio Pisces Teleostei
spellingShingle	Carbon Dioxide Hemoglobin Proton Acid Base Balance Acidity Animals Cell Animals Experiment Blood Oxygenation Bohr Effect Carbon Dioxide Blood Level Carp Cell Ph Cell Suspension Constants And Coefficients Controlled Study Erythrocyte Evaluation Gas Transport Hematological Parameters In Vivo Study Nonhuman Nonlinear System Oxygen Saturation Oxygen Tension Proton Transport Species Difference Teleost Animalsia Colossoma Macropomum Colossoma Marcopomum Cyprinus Carpio Pisces Teleostei Brauner, Colin John Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
topic_facet	Carbon Dioxide Hemoglobin Proton Acid Base Balance Acidity Animals Cell Animals Experiment Blood Oxygenation Bohr Effect Carbon Dioxide Blood Level Carp Cell Ph Cell Suspension Constants And Coefficients Controlled Study Erythrocyte Evaluation Gas Transport Hematological Parameters In Vivo Study Nonhuman Nonlinear System Oxygen Saturation Oxygen Tension Proton Transport Species Difference Teleost Animalsia Colossoma Macropomum Colossoma Marcopomum Cyprinus Carpio Pisces Teleostei
description	Some teleost fishes exhibit a non-linear release of H+ with haemoglobin oxygen saturation (SO2) in whole blood that may be related to the Root effect and a low apparent cooperativity in oxygen binding. To further investigate this correlation, the relationship between red cell pH (pHi) and SO2 was evaluated in two teleost fishes, the carp (Cyprinus carpio) and tambaqui (Colossoma macropomum). Carp possess a relatively small Root effect within the physiological pH range, while that in tambaqui is relatively large. It was therefore hypothesized that both species would possess a non-linear release of H+ with SO2 under in vivo conditions, but that the degree of non-linearity would be less pronounced in carp than in tambaqui. Red cell suspensions of tambaqui showed a marked non-linear relationship between pHi and SO2 at in vivo pH values (pHe = 7.66), where the majority of Bohr protons were released between 50 and 100% SO2. In whole blood of carp, the relationship between pHi and SO2 was almost linear at normal resting extracellular pH values (pHe = 8.10) where Hills n50 (cooperativity) was 1.3. Under acidic conditions (pHe = 7.11), n50 decreased to 0.9 and the release of H+ with SO2 became slightly non-linear. The reduction in pH in carp blood was associated with the onset of the Root effect, and the oxygen tension at half saturation (P50) increased from 3.8 to 38.2 mmHg. The relationship between total CO2 and SO2 in carp whole blood changed from being almost linear at pHe 8.1 to being non-linear at pHe 7.1, consistent with the relationship between pHi and SO2. Thus, possession of a Root effect could be a prerequisite for a non-linear release of Bohr protons with oxygenation, but the expression in whole blood may depend upon a species-specific cooperativity threshold. The non-linear release of Bohr protons with oxygenation may be a general phenomenon in teleosts with important implications for gas transport and acid-base homeostasis.
format	Artigo
author	Brauner, Colin John
author2	Wang, Tobias Val, Adalberto Luis Jensen, Frank Bo
author2Str	Wang, Tobias Val, Adalberto Luis Jensen, Frank Bo
title	Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
title_short	Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
title_full	Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
title_fullStr	Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
title_full_unstemmed	Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)
title_sort	non-linear release of bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (cyprinus carpio) and tambaqui (colossoma macropomum)
publisher	Fish Physiology and Biochemistry
publishDate	2020
url	https://repositorio.inpa.gov.br/handle/1/19104
_version_	1787141157706268672
score	11.755432

Non-linear release of Bohr protons with haemoglobin-oxygenation in the blood of two teleost fishes; carp (Cyprinus carpio) and tambaqui (Colossoma macropomum)

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