No co-expression of LDH-C in amazon cichlids
As commonly found among other advanced telcosts, lactate dehydrogenase (LDH, EC. 1.1.1.27) from cichlid fish exhibits the eye-specific LDH-C4 isozyme. In the last few yeats, some authors have described the co-expression of a distinct liver-specific form in addition to the eye-specific LDH isozymes i...
| Autor principal: | Farias, Izeni P. |
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| Outros Autores: | Paula-Silva, Maria Nazaré N., Almeida-Val, Vera Maria Fonseca |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19350 |
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oai:repositorio:1-19350 |
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oai:repositorio:1-19350 No co-expression of LDH-C in amazon cichlids Farias, Izeni P. Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca Alcohol Dehydrogenase Enzyme Inhibitor Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Animals Tissue Enzyme Activity Eye Fish Gel Electrophoresis Liver Nonhuman Priority Journal Protein Expression Animalsia Cichlidae Gadiformes Nassa Pisces As commonly found among other advanced telcosts, lactate dehydrogenase (LDH, EC. 1.1.1.27) from cichlid fish exhibits the eye-specific LDH-C4 isozyme. In the last few yeats, some authors have described the co-expression of a distinct liver-specific form in addition to the eye-specific LDH isozymes in cichlid fish. Because liver-specific isozymes have been described before in Gadiformes as the product of the same LDH-C*locus, such. co- expression of these isozymes was explained as the result of a fourth locus for LDH. Our studies on Amazon cichlid fishes revealed that the specific isozyme present in liver is the result of activity of another enzyme: alcohol dehydrogenase (ADH, EC. 1.I.1.1.). Several tests with ADH specific staining and the use of ADH inhibitors confirmed that the isozyme described as LDH in liver of Acaron/a nassa is actually the result of ADH activity. It is common during the staining procedure for LDH and other dehydrogenases such as malate dehydrogenase (EC. 1.1.1.37) for the ADH isozyme to also appear. Considering these results, we suggest that LDH occurs as the product of three loci (LDH- A* LDH-B* and LDH-C*) in these fish. 2020-06-15T22:07:42Z 2020-06-15T22:07:42Z 1997 Artigo https://repositorio.inpa.gov.br/handle/1/19350 10.1016/S0305-0491(96)00324-0 en Volume 117, Número 2, Pags. 315-319 Restrito Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Alcohol Dehydrogenase Enzyme Inhibitor Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Animals Tissue Enzyme Activity Eye Fish Gel Electrophoresis Liver Nonhuman Priority Journal Protein Expression Animalsia Cichlidae Gadiformes Nassa Pisces |
| spellingShingle |
Alcohol Dehydrogenase Enzyme Inhibitor Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Animals Tissue Enzyme Activity Eye Fish Gel Electrophoresis Liver Nonhuman Priority Journal Protein Expression Animalsia Cichlidae Gadiformes Nassa Pisces Farias, Izeni P. No co-expression of LDH-C in amazon cichlids |
| topic_facet |
Alcohol Dehydrogenase Enzyme Inhibitor Isoenzyme Lactate Dehydrogenase Malate Dehydrogenase Animals Tissue Enzyme Activity Eye Fish Gel Electrophoresis Liver Nonhuman Priority Journal Protein Expression Animalsia Cichlidae Gadiformes Nassa Pisces |
| description |
As commonly found among other advanced telcosts, lactate dehydrogenase (LDH, EC. 1.1.1.27) from cichlid fish exhibits the eye-specific LDH-C4 isozyme. In the last few yeats, some authors have described the co-expression of a distinct liver-specific form in addition to the eye-specific LDH isozymes in cichlid fish. Because liver-specific isozymes have been described before in Gadiformes as the product of the same LDH-C*locus, such. co- expression of these isozymes was explained as the result of a fourth locus for LDH. Our studies on Amazon cichlid fishes revealed that the specific isozyme present in liver is the result of activity of another enzyme: alcohol dehydrogenase (ADH, EC. 1.I.1.1.). Several tests with ADH specific staining and the use of ADH inhibitors confirmed that the isozyme described as LDH in liver of Acaron/a nassa is actually the result of ADH activity. It is common during the staining procedure for LDH and other dehydrogenases such as malate dehydrogenase (EC. 1.1.1.37) for the ADH isozyme to also appear. Considering these results, we suggest that LDH occurs as the product of three loci (LDH- A* LDH-B* and LDH-C*) in these fish. |
| format |
Artigo |
| author |
Farias, Izeni P. |
| author2 |
Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca |
| author2Str |
Paula-Silva, Maria Nazaré N. Almeida-Val, Vera Maria Fonseca |
| title |
No co-expression of LDH-C in amazon cichlids |
| title_short |
No co-expression of LDH-C in amazon cichlids |
| title_full |
No co-expression of LDH-C in amazon cichlids |
| title_fullStr |
No co-expression of LDH-C in amazon cichlids |
| title_full_unstemmed |
No co-expression of LDH-C in amazon cichlids |
| title_sort |
no co-expression of ldh-c in amazon cichlids |
| publisher |
Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19350 |
| _version_ |
1787144361084977152 |
| score |
11.653393 |