Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data
In this study, we have analysed enzyme polymorphism among a group of protozoan parasites of the genus Endotrypanum (Kinetoplastida: Tryganosomatidae). Seventeen stocks of Endotrypanum spp. isolated from sloths (Choloepus didactylus and C. juruanus) in the Amazon Region of Brazil were analysed by enz...
| Autor principal: | Franco, A. M. |
|---|---|
| Outros Autores: | Momen, Hooman, Naiff, Roberto Daibes, Moreira, Celia F.S., Deane, Maria Paumgartten, Grimaldi Júnior, Gabriel F. |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Parasitology
2020
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| Assuntos: | |
| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19384 |
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oai:repositorio:1-19384 |
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oai:repositorio:1-19384 Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data Franco, A. M. Momen, Hooman Naiff, Roberto Daibes Moreira, Celia F.S. Deane, Maria Paumgartten Grimaldi Júnior, Gabriel F. Isoenzyme Unclassified Drug Animals Experiment Brasil Controlled Study Electrophoresis Enzyme Analysis Flagellate Polymorphism, Genetic Nonhuman Priority Journal Zymodeme Animal Brasil Cluster Analysis Electrophoresis, Agar Gel Gene Frequency Isoenzymes Phenotype Phylogeny Polymorphism, Genetic Sloths Software Trypanosomatina Animalsia Choloepus Didactylus Edentata Endotrypanum Endotrypanum Monterogeii Endotrypanum Schaudinni Kinetoplastida Mastigophora (flagellates) Protozoa Rondonia In this study, we have analysed enzyme polymorphism among a group of protozoan parasites of the genus Endotrypanum (Kinetoplastida: Tryganosomatidae). Seventeen stocks of Endotrypanum spp. isolated from sloths (Choloepus didactylus and C. juruanus) in the Amazon Region of Brazil were analysed by enzyme electrophoresis, and their electromorphic profiles were compared with reference strains reported previously. The 16 enzymic loci were analysed, and the strains were classified into zymodemes, each representing parasites with unique enzyme profiles. Each zymodeme was considered as an elementary taxon, and using numerical analyses (cladistic, agglomerative hierarchical and ordination techniques) the genus was shown to be monophyletic and the 12 zymodemes characterized could be divided into 3 groups (A, B, C). The heterogeneous population (which may represent a complex of parasite species or strains variants) showed, however, no correlation with the origin (i.e, host species involved or geographic area of isolation) of Endotrypanum stocks. Eight isolates of Endotrypanum sp. from Rondonia State (Brazil) and a parasite strain from Panama were clustered together into a zymodeme, which was phenetically closely related to the E. monterogeii from Costa Rica. The data indicate that E. schaudinni is a species complex. 2020-06-15T22:08:03Z 2020-06-15T22:08:03Z 1996 Artigo https://repositorio.inpa.gov.br/handle/1/19384 en Volume 113, Número 1, Pags. 39-48 Restrito Parasitology |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Isoenzyme Unclassified Drug Animals Experiment Brasil Controlled Study Electrophoresis Enzyme Analysis Flagellate Polymorphism, Genetic Nonhuman Priority Journal Zymodeme Animal Brasil Cluster Analysis Electrophoresis, Agar Gel Gene Frequency Isoenzymes Phenotype Phylogeny Polymorphism, Genetic Sloths Software Trypanosomatina Animalsia Choloepus Didactylus Edentata Endotrypanum Endotrypanum Monterogeii Endotrypanum Schaudinni Kinetoplastida Mastigophora (flagellates) Protozoa Rondonia |
| spellingShingle |
Isoenzyme Unclassified Drug Animals Experiment Brasil Controlled Study Electrophoresis Enzyme Analysis Flagellate Polymorphism, Genetic Nonhuman Priority Journal Zymodeme Animal Brasil Cluster Analysis Electrophoresis, Agar Gel Gene Frequency Isoenzymes Phenotype Phylogeny Polymorphism, Genetic Sloths Software Trypanosomatina Animalsia Choloepus Didactylus Edentata Endotrypanum Endotrypanum Monterogeii Endotrypanum Schaudinni Kinetoplastida Mastigophora (flagellates) Protozoa Rondonia Franco, A. M. Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| topic_facet |
Isoenzyme Unclassified Drug Animals Experiment Brasil Controlled Study Electrophoresis Enzyme Analysis Flagellate Polymorphism, Genetic Nonhuman Priority Journal Zymodeme Animal Brasil Cluster Analysis Electrophoresis, Agar Gel Gene Frequency Isoenzymes Phenotype Phylogeny Polymorphism, Genetic Sloths Software Trypanosomatina Animalsia Choloepus Didactylus Edentata Endotrypanum Endotrypanum Monterogeii Endotrypanum Schaudinni Kinetoplastida Mastigophora (flagellates) Protozoa Rondonia |
| description |
In this study, we have analysed enzyme polymorphism among a group of protozoan parasites of the genus Endotrypanum (Kinetoplastida: Tryganosomatidae). Seventeen stocks of Endotrypanum spp. isolated from sloths (Choloepus didactylus and C. juruanus) in the Amazon Region of Brazil were analysed by enzyme electrophoresis, and their electromorphic profiles were compared with reference strains reported previously. The 16 enzymic loci were analysed, and the strains were classified into zymodemes, each representing parasites with unique enzyme profiles. Each zymodeme was considered as an elementary taxon, and using numerical analyses (cladistic, agglomerative hierarchical and ordination techniques) the genus was shown to be monophyletic and the 12 zymodemes characterized could be divided into 3 groups (A, B, C). The heterogeneous population (which may represent a complex of parasite species or strains variants) showed, however, no correlation with the origin (i.e, host species involved or geographic area of isolation) of Endotrypanum stocks. Eight isolates of Endotrypanum sp. from Rondonia State (Brazil) and a parasite strain from Panama were clustered together into a zymodeme, which was phenetically closely related to the E. monterogeii from Costa Rica. The data indicate that E. schaudinni is a species complex. |
| format |
Artigo |
| author |
Franco, A. M. |
| author2 |
Momen, Hooman Naiff, Roberto Daibes Moreira, Celia F.S. Deane, Maria Paumgartten Grimaldi Júnior, Gabriel F. |
| author2Str |
Momen, Hooman Naiff, Roberto Daibes Moreira, Celia F.S. Deane, Maria Paumgartten Grimaldi Júnior, Gabriel F. |
| title |
Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| title_short |
Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| title_full |
Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| title_fullStr |
Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| title_full_unstemmed |
Enzyme polymorphism in Endotrypanum and numerical analysis of isoenzyme data |
| title_sort |
enzyme polymorphism in endotrypanum and numerical analysis of isoenzyme data |
| publisher |
Parasitology |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19384 |
| _version_ |
1787144733932388352 |
| score |
11.755432 |