Biochemical adjustments to hypoxia by Amazon cichlids.
The isozyme distribution of cichlid lactate dehydrogenase (LDH) is related to species environmental preferences. Cichlasoma amazonarum occurs in different environments and presents LDH tissue distribution patterns that correlate with oxygen tension at the capture location. Cichlasoma amazonarum was...
| Autor principal: | Almeida-Val, Vera Maria Fonseca |
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| Outros Autores: | Farias, Izeni P., Silva, M. N.P., Duncan, Wallice Luiz Paxiuba, Val, Adalberto Luis |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.]
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/19432 |
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oai:repositorio:1-19432 |
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dspace |
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oai:repositorio:1-19432 Biochemical adjustments to hypoxia by Amazon cichlids. Almeida-Val, Vera Maria Fonseca Farias, Izeni P. Silva, M. N.P. Duncan, Wallice Luiz Paxiuba Val, Adalberto Luis Isoenzyme Lactate Dehydrogenase Analysis Of Variance Animals Anoxia Brain Electrophoresis Enzymology Heart Muscle Liver Metabolism Perch Review Muscle, Skeletal Analysis Of Variance Animal Anoxia Brain Electrophoresis Isoenzymes L-lactate Dehydrogenase Liver Muscle, Skeletal Myocardium Perches The isozyme distribution of cichlid lactate dehydrogenase (LDH) is related to species environmental preferences. Cichlasoma amazonarum occurs in different environments and presents LDH tissue distribution patterns that correlate with oxygen tension at the capture location. Cichlasoma amazonarum was exposed to long-term severe hypoxia (51 days at 36.4 +/- 5.9 mmHg), tissue LDH isozyme distribution was analyzed by electrophoresis and enzyme activities were measured by monitoring the oxidation of NADH as pyruvate was reduced to lactate. The exposure of Cichlasoma amazonarum to long-term severe hypoxia resulted in changes in the tissue distribution of LDH isozymes. The major changes in response to hypoxia occurred in heart, liver and brain: isozyme A4 was activated in heart and brain, whereas isozyme B4 was activated in liver. The most significant quantitative change occurred in brain LDH of hypoxia-exposed animals which adopted muscle type kinetics, reflecting a new LDH isozyme distribution. LDH activity was significantly reduced (P < 0.05) in animals exposed to hypoxia (N = 8), suggesting an overall LDH suppression. Pyruvate inhibition decreased in all hypoxia-exposed tissues. Thus, the ability of Cichlasoma amazonarum to regulate LDH tissue expression according to oxygen availability allows the animal to survive chronic hypoxic environments. This phenotypic plasticity may occur in other hypoxia-tolerant fish species. 2020-06-15T22:08:36Z 2020-06-15T22:08:36Z 1995 Artigo https://repositorio.inpa.gov.br/handle/1/19432 en Volume 28, Número 11-12, Pags. 1257-1263 Restrito Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Isoenzyme Lactate Dehydrogenase Analysis Of Variance Animals Anoxia Brain Electrophoresis Enzymology Heart Muscle Liver Metabolism Perch Review Muscle, Skeletal Analysis Of Variance Animal Anoxia Brain Electrophoresis Isoenzymes L-lactate Dehydrogenase Liver Muscle, Skeletal Myocardium Perches |
| spellingShingle |
Isoenzyme Lactate Dehydrogenase Analysis Of Variance Animals Anoxia Brain Electrophoresis Enzymology Heart Muscle Liver Metabolism Perch Review Muscle, Skeletal Analysis Of Variance Animal Anoxia Brain Electrophoresis Isoenzymes L-lactate Dehydrogenase Liver Muscle, Skeletal Myocardium Perches Almeida-Val, Vera Maria Fonseca Biochemical adjustments to hypoxia by Amazon cichlids. |
| topic_facet |
Isoenzyme Lactate Dehydrogenase Analysis Of Variance Animals Anoxia Brain Electrophoresis Enzymology Heart Muscle Liver Metabolism Perch Review Muscle, Skeletal Analysis Of Variance Animal Anoxia Brain Electrophoresis Isoenzymes L-lactate Dehydrogenase Liver Muscle, Skeletal Myocardium Perches |
| description |
The isozyme distribution of cichlid lactate dehydrogenase (LDH) is related to species environmental preferences. Cichlasoma amazonarum occurs in different environments and presents LDH tissue distribution patterns that correlate with oxygen tension at the capture location. Cichlasoma amazonarum was exposed to long-term severe hypoxia (51 days at 36.4 +/- 5.9 mmHg), tissue LDH isozyme distribution was analyzed by electrophoresis and enzyme activities were measured by monitoring the oxidation of NADH as pyruvate was reduced to lactate. The exposure of Cichlasoma amazonarum to long-term severe hypoxia resulted in changes in the tissue distribution of LDH isozymes. The major changes in response to hypoxia occurred in heart, liver and brain: isozyme A4 was activated in heart and brain, whereas isozyme B4 was activated in liver. The most significant quantitative change occurred in brain LDH of hypoxia-exposed animals which adopted muscle type kinetics, reflecting a new LDH isozyme distribution. LDH activity was significantly reduced (P < 0.05) in animals exposed to hypoxia (N = 8), suggesting an overall LDH suppression. Pyruvate inhibition decreased in all hypoxia-exposed tissues. Thus, the ability of Cichlasoma amazonarum to regulate LDH tissue expression according to oxygen availability allows the animal to survive chronic hypoxic environments. This phenotypic plasticity may occur in other hypoxia-tolerant fish species. |
| format |
Artigo |
| author |
Almeida-Val, Vera Maria Fonseca |
| author2 |
Farias, Izeni P. Silva, M. N.P. Duncan, Wallice Luiz Paxiuba Val, Adalberto Luis |
| author2Str |
Farias, Izeni P. Silva, M. N.P. Duncan, Wallice Luiz Paxiuba Val, Adalberto Luis |
| title |
Biochemical adjustments to hypoxia by Amazon cichlids. |
| title_short |
Biochemical adjustments to hypoxia by Amazon cichlids. |
| title_full |
Biochemical adjustments to hypoxia by Amazon cichlids. |
| title_fullStr |
Biochemical adjustments to hypoxia by Amazon cichlids. |
| title_full_unstemmed |
Biochemical adjustments to hypoxia by Amazon cichlids. |
| title_sort |
biochemical adjustments to hypoxia by amazon cichlids. |
| publisher |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas / Sociedade Brasileira de Biofísica ... [et al.] |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/19432 |
| _version_ |
1787144148770357248 |
| score |
11.675608 |