Artigo

LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)

1. 1. The patterns of distribution of LDH isozymes in five tissues from 12 Serrasalmidae species have been studied through two-fold serial dilution (Klebe's test) as well as the pyruvate inhibition of LDH enzyme in skeletal and heart muscles (low/high ratios). 2. 2. The species' electrophoretic patt...

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Autor principal: Almeida-Val, Vera Maria Fonseca
Outros Autores: Silva, Maria de Nazaré Paula da, Magalhães Caraciolo, Mércia Cristina de, Mesquita, Lenise Socorro Benarrós de, Farias, Izeni P., Val, Adalberto Luis
Grau: Artigo
Idioma: English
Publicado em: Comparative Biochemistry and Physiology -- Part B: Biochemistry and 2020
Assuntos:
Isoenzyme
Lactate Dehydrogenase
Animals Tissue
Enzyme Localization
Fish
Nonhuman
Priority Journal
Tissue Distribution
Animalsia
Ostariophysi
Pisces
Serrasalmidae
Teleostei
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/19525
id oai:repositorio:1-19525
recordtype dspace
spelling oai:repositorio:1-19525 LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi) Almeida-Val, Vera Maria Fonseca Silva, Maria de Nazaré Paula da Magalhães Caraciolo, Mércia Cristina de Mesquita, Lenise Socorro Benarrós de Farias, Izeni P. Val, Adalberto Luis Isoenzyme Lactate Dehydrogenase Animals Tissue Enzyme Localization Fish Nonhuman Priority Journal Tissue Distribution Animalsia Ostariophysi Pisces Serrasalmidae Teleostei 1. 1. The patterns of distribution of LDH isozymes in five tissues from 12 Serrasalmidae species have been studied through two-fold serial dilution (Klebe's test) as well as the pyruvate inhibition of LDH enzyme in skeletal and heart muscles (low/high ratios). 2. 2. The species' electrophoretic patterns differ by orthologous A4 isozyme mobilities since the orthologous B4 isozymes present similar electrophoretic migration. These differences between Ldh-A and Ldh-B products reflect three-, four-, and five-banded patterns. Thus, different LDH isozyme numbers formed from A and B subunits should not be used as an evolutionary or phylogenetic characteristic from different taxonomic groups. 3. 3. Out of 66 pairs of species only five pairs showed significant differences in the distribution patterns in all five analyzed tissues, while no pair of species showed the same distribution in these tissues. This variation was explained as differential regulation of structural genes among tissues and/or species. 4. 4. Functional properties showed significant between the LDHs from heart and skeletal muscles, and are consistent with a preference for aerobic metabolism. We suppose that by selecting B-like subunits these fishes are able to maintain good control of aerobic/anaerobic transitions, maintaining predominantly oxidative metabolism even in hypoxic waters, with which they have to cope. © 1992. 2020-06-15T22:09:33Z 2020-06-15T22:09:33Z 1992 Artigo https://repositorio.inpa.gov.br/handle/1/19525 10.1016/0305-0491(92)90422-N en Volume 103, Número 1, Pags. 119-125 Restrito Comparative Biochemistry and Physiology -- Part B: Biochemistry and
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
topic Isoenzyme
Lactate Dehydrogenase
Animals Tissue
Enzyme Localization
Fish
Nonhuman
Priority Journal
Tissue Distribution
Animalsia
Ostariophysi
Pisces
Serrasalmidae
Teleostei
spellingShingle Isoenzyme
Lactate Dehydrogenase
Animals Tissue
Enzyme Localization
Fish
Nonhuman
Priority Journal
Tissue Distribution
Animalsia
Ostariophysi
Pisces
Serrasalmidae
Teleostei
Almeida-Val, Vera Maria Fonseca
LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
topic_facet Isoenzyme
Lactate Dehydrogenase
Animals Tissue
Enzyme Localization
Fish
Nonhuman
Priority Journal
Tissue Distribution
Animalsia
Ostariophysi
Pisces
Serrasalmidae
Teleostei
description 1. 1. The patterns of distribution of LDH isozymes in five tissues from 12 Serrasalmidae species have been studied through two-fold serial dilution (Klebe's test) as well as the pyruvate inhibition of LDH enzyme in skeletal and heart muscles (low/high ratios). 2. 2. The species' electrophoretic patterns differ by orthologous A4 isozyme mobilities since the orthologous B4 isozymes present similar electrophoretic migration. These differences between Ldh-A and Ldh-B products reflect three-, four-, and five-banded patterns. Thus, different LDH isozyme numbers formed from A and B subunits should not be used as an evolutionary or phylogenetic characteristic from different taxonomic groups. 3. 3. Out of 66 pairs of species only five pairs showed significant differences in the distribution patterns in all five analyzed tissues, while no pair of species showed the same distribution in these tissues. This variation was explained as differential regulation of structural genes among tissues and/or species. 4. 4. Functional properties showed significant between the LDHs from heart and skeletal muscles, and are consistent with a preference for aerobic metabolism. We suppose that by selecting B-like subunits these fishes are able to maintain good control of aerobic/anaerobic transitions, maintaining predominantly oxidative metabolism even in hypoxic waters, with which they have to cope. © 1992.
format Artigo
author Almeida-Val, Vera Maria Fonseca
author2 Silva, Maria de Nazaré Paula da
Magalhães Caraciolo, Mércia Cristina de
Mesquita, Lenise Socorro Benarrós de
Farias, Izeni P.
Val, Adalberto Luis
author2Str Silva, Maria de Nazaré Paula da
Magalhães Caraciolo, Mércia Cristina de
Mesquita, Lenise Socorro Benarrós de
Farias, Izeni P.
Val, Adalberto Luis
title LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
title_short LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
title_full LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
title_fullStr LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
title_full_unstemmed LDH isozymes in amazon fish-III. Distribution patterns and functional properties in Serrasalmidae (Teleostei: Ostariophysi)
title_sort ldh isozymes in amazon fish-iii. distribution patterns and functional properties in serrasalmidae (teleostei: ostariophysi)
publisher Comparative Biochemistry and Physiology -- Part B: Biochemistry and
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/19525
_version_ 1787141499481227264
score 11.675608

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