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Artigo
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, sugges...
Autor principal: | Farias, Izeni P. |
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Outros Autores: | Almeida-Val, Vera Maria Fonseca |
Grau: | Artigo |
Idioma: | English |
Publicado em: |
Comparative Biochemistry and Physiology -- Part B: Biochemistry and
2020
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https://repositorio.inpa.gov.br/handle/1/19526 |
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oai:repositorio:1-19526 |
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oai:repositorio:1-19526 Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features Farias, Izeni P. Almeida-Val, Vera Maria Fonseca Isoenzyme Malate Dehydrogenase Animals Tissue Evolution Fish Gene Duplication Gene Locus Nonhuman Priority Journal Protein Electrophoresis Species Difference Animalsia Astronotus Ocellatus Cichla Cichlidae Geophagus Mesonauta Insignis Pisces 1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, suggesting the existence of three gene loci (sMDH-A*, sMDH-B1* and sMDH-B2*). 2. 2. Klebe's serial dilutions, thermostability tests and tissue specificity performed on the sMDH of studied species indicated no divergence between B1* and B2* loci products, suggesting that these genes probably undergo the same regulatory gene action and that the duplication event occurred recently, after A* and B* divergence. 3. 3. The appearance of the same characteristics in all specimens, and the chromosomic picture of the family, suggest the occurrence of an event of duplication "in tandem" in the ancestors of Amazon cichlids. © 1992. 2020-06-15T22:09:33Z 2020-06-15T22:09:33Z 1992 Artigo https://repositorio.inpa.gov.br/handle/1/19526 10.1016/0305-0491(92)90219-H en Volume 103, Número 4, Pags. 939-943 Restrito Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
collection |
INPA-RI |
language |
English |
topic |
Isoenzyme Malate Dehydrogenase Animals Tissue Evolution Fish Gene Duplication Gene Locus Nonhuman Priority Journal Protein Electrophoresis Species Difference Animalsia Astronotus Ocellatus Cichla Cichlidae Geophagus Mesonauta Insignis Pisces |
spellingShingle |
Isoenzyme Malate Dehydrogenase Animals Tissue Evolution Fish Gene Duplication Gene Locus Nonhuman Priority Journal Protein Electrophoresis Species Difference Animalsia Astronotus Ocellatus Cichla Cichlidae Geophagus Mesonauta Insignis Pisces Farias, Izeni P. Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
topic_facet |
Isoenzyme Malate Dehydrogenase Animals Tissue Evolution Fish Gene Duplication Gene Locus Nonhuman Priority Journal Protein Electrophoresis Species Difference Animalsia Astronotus Ocellatus Cichla Cichlidae Geophagus Mesonauta Insignis Pisces |
description |
1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, suggesting the existence of three gene loci (sMDH-A*, sMDH-B1* and sMDH-B2*). 2. 2. Klebe's serial dilutions, thermostability tests and tissue specificity performed on the sMDH of studied species indicated no divergence between B1* and B2* loci products, suggesting that these genes probably undergo the same regulatory gene action and that the duplication event occurred recently, after A* and B* divergence. 3. 3. The appearance of the same characteristics in all specimens, and the chromosomic picture of the family, suggest the occurrence of an event of duplication "in tandem" in the ancestors of Amazon cichlids. © 1992. |
format |
Artigo |
author |
Farias, Izeni P. |
author2 |
Almeida-Val, Vera Maria Fonseca |
author2Str |
Almeida-Val, Vera Maria Fonseca |
title |
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
title_short |
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
title_full |
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
title_fullStr |
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
title_full_unstemmed |
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features |
title_sort |
malate dehydrogenase (smdh) in amazon cichlid fishes: evolutionary features |
publisher |
Comparative Biochemistry and Physiology -- Part B: Biochemistry and |
publishDate |
2020 |
url |
https://repositorio.inpa.gov.br/handle/1/19526 |
_version_ |
1787145289656696832 |
score |
11.755432 |