Artigo

Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features

1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, sugges...

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Autor principal: Farias, Izeni P.
Outros Autores: Almeida-Val, Vera Maria Fonseca
Grau: Artigo
Idioma: English
Publicado em: Comparative Biochemistry and Physiology -- Part B: Biochemistry and 2020
Assuntos:
Acesso em linha: https://repositorio.inpa.gov.br/handle/1/19526
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spelling oai:repositorio:1-19526 Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features Farias, Izeni P. Almeida-Val, Vera Maria Fonseca Isoenzyme Malate Dehydrogenase Animals Tissue Evolution Fish Gene Duplication Gene Locus Nonhuman Priority Journal Protein Electrophoresis Species Difference Animalsia Astronotus Ocellatus Cichla Cichlidae Geophagus Mesonauta Insignis Pisces 1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, suggesting the existence of three gene loci (sMDH-A*, sMDH-B1* and sMDH-B2*). 2. 2. Klebe's serial dilutions, thermostability tests and tissue specificity performed on the sMDH of studied species indicated no divergence between B1* and B2* loci products, suggesting that these genes probably undergo the same regulatory gene action and that the duplication event occurred recently, after A* and B* divergence. 3. 3. The appearance of the same characteristics in all specimens, and the chromosomic picture of the family, suggest the occurrence of an event of duplication "in tandem" in the ancestors of Amazon cichlids. © 1992. 2020-06-15T22:09:33Z 2020-06-15T22:09:33Z 1992 Artigo https://repositorio.inpa.gov.br/handle/1/19526 10.1016/0305-0491(92)90219-H en Volume 103, Número 4, Pags. 939-943 Restrito Comparative Biochemistry and Physiology -- Part B: Biochemistry and
institution Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional
collection INPA-RI
language English
topic Isoenzyme
Malate Dehydrogenase
Animals Tissue
Evolution
Fish
Gene Duplication
Gene Locus
Nonhuman
Priority Journal
Protein Electrophoresis
Species Difference
Animalsia
Astronotus Ocellatus
Cichla
Cichlidae
Geophagus
Mesonauta Insignis
Pisces
spellingShingle Isoenzyme
Malate Dehydrogenase
Animals Tissue
Evolution
Fish
Gene Duplication
Gene Locus
Nonhuman
Priority Journal
Protein Electrophoresis
Species Difference
Animalsia
Astronotus Ocellatus
Cichla
Cichlidae
Geophagus
Mesonauta Insignis
Pisces
Farias, Izeni P.
Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
topic_facet Isoenzyme
Malate Dehydrogenase
Animals Tissue
Evolution
Fish
Gene Duplication
Gene Locus
Nonhuman
Priority Journal
Protein Electrophoresis
Species Difference
Animalsia
Astronotus Ocellatus
Cichla
Cichlidae
Geophagus
Mesonauta Insignis
Pisces
description 1. 1. The sMDH isozyme system was studied in five species of cichlid fishes found in the Amazon hydrographic basin (Astronotus ocellatus, Cichla monoculus, Geophagus cff harreri, Cichlassoma severum and Mesonauta insignis). All studied specimens presented a six-banded electrophoretic pattern, suggesting the existence of three gene loci (sMDH-A*, sMDH-B1* and sMDH-B2*). 2. 2. Klebe's serial dilutions, thermostability tests and tissue specificity performed on the sMDH of studied species indicated no divergence between B1* and B2* loci products, suggesting that these genes probably undergo the same regulatory gene action and that the duplication event occurred recently, after A* and B* divergence. 3. 3. The appearance of the same characteristics in all specimens, and the chromosomic picture of the family, suggest the occurrence of an event of duplication "in tandem" in the ancestors of Amazon cichlids. © 1992.
format Artigo
author Farias, Izeni P.
author2 Almeida-Val, Vera Maria Fonseca
author2Str Almeida-Val, Vera Maria Fonseca
title Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
title_short Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
title_full Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
title_fullStr Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
title_full_unstemmed Malate dehydrogenase (sMDH) in Amazon cichlid fishes: evolutionary features
title_sort malate dehydrogenase (smdh) in amazon cichlid fishes: evolutionary features
publisher Comparative Biochemistry and Physiology -- Part B: Biochemistry and
publishDate 2020
url https://repositorio.inpa.gov.br/handle/1/19526
_version_ 1787145289656696832
score 11.755432