Development of a portable leaf photosynthesis and volatile organic compounds emission system
Understanding how plant carbon metabolism responds to environmental variables such as light is central to understanding ecosystem carbon cycling and the production of food, biofuels, and biomaterials. Here, we couple a portable leaf photosynthesis system to an autosampler for volatile organic compou...
| Autor principal: | Jardine, Kolby J. |
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| Outros Autores: | Zorzanelli, Raquel F., Gimenez, Bruno Oliva, Robles, Emily, Oliveira Piva, Luani Rosa de |
| Grau: | Artigo |
| Idioma: | English |
| Publicado em: |
MethodsX
2020
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| Acesso em linha: |
https://repositorio.inpa.gov.br/handle/1/23315 |
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oai:repositorio:1-23315 |
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oai:repositorio:1-23315 Development of a portable leaf photosynthesis and volatile organic compounds emission system Jardine, Kolby J. Zorzanelli, Raquel F. Gimenez, Bruno Oliva Robles, Emily Oliveira Piva, Luani Rosa de Carbon Dioxide Monoterpene Volatile Organic Compound Water automation Banana Carbon Cycling Carbon Metabolism desorption Environmental Temperature Light Intensity Mass Fragmentography Photosynthesis Plant Leaf Plant Physiology Priority Journal Tropical Rain Forest Understanding how plant carbon metabolism responds to environmental variables such as light is central to understanding ecosystem carbon cycling and the production of food, biofuels, and biomaterials. Here, we couple a portable leaf photosynthesis system to an autosampler for volatile organic compounds (VOCs) to enable field observations of net photosynthesis simultaneously with emissions of VOCs as a function of light. Following sample collection, VOCs are analyzed using automated thermal desorption-gas chromatograph-mass spectrometry (TD-GC–MS). An example is presented from a banana plant in the central Amazon with a focus on the response of photosynthesis and the emissions of eight individual monoterpenes to light intensity. Our observations reveal that banana leaf emissions represent a 1.1 +/- 0.1% loss of photosynthesis by carbon. Monoterpene emissions from banana are dominated by trans-β-ocimene, which accounts for up to 57% of total monoterpene emissions at high light. We conclude that the developed system is ideal for the identification and quantification of VOC emissions from leaves in parallel with CO2 and water fluxes.The system therefore permits the analysis of biological and environmental sensitivities of carbon metabolism in leaves in remote field locations, resulting in the emission of hydrocarbons to the atmosphere. • A field-portable system is developed for the identification and quantification of VOCs from leaves in parallel with leaf physiological measurements including photosynthesis and transpiration. • The system will enable the characterization of carbon and energy allocation to the biosynthesis and emission of VOCs linked with photosynthesis (e.g. isoprene and monoterpenes) and their biological and environmental sensitivities (e.g. light, temperature, CO2). • Allow the development of more accurate mechanistic global VOC emission models linked with photosynthesis, improving our ability to predict how forests will respond to climate change. It is our hope that the presented system will contribute with critical data towards these goals across Earth's diverse tropical forests. © 2020 2020-07-03T22:30:26Z 2020-07-03T22:30:26Z 2020 Artigo https://repositorio.inpa.gov.br/handle/1/23315 10.1016/j.mex.2020.100880 en Volume 7 Attribution-NonCommercial-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nc-nd/3.0/br/ MethodsX |
| institution |
Instituto Nacional de Pesquisas da Amazônia - Repositório Institucional |
| collection |
INPA-RI |
| language |
English |
| topic |
Carbon Dioxide Monoterpene Volatile Organic Compound Water automation Banana Carbon Cycling Carbon Metabolism desorption Environmental Temperature Light Intensity Mass Fragmentography Photosynthesis Plant Leaf Plant Physiology Priority Journal Tropical Rain Forest |
| spellingShingle |
Carbon Dioxide Monoterpene Volatile Organic Compound Water automation Banana Carbon Cycling Carbon Metabolism desorption Environmental Temperature Light Intensity Mass Fragmentography Photosynthesis Plant Leaf Plant Physiology Priority Journal Tropical Rain Forest Jardine, Kolby J. Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| topic_facet |
Carbon Dioxide Monoterpene Volatile Organic Compound Water automation Banana Carbon Cycling Carbon Metabolism desorption Environmental Temperature Light Intensity Mass Fragmentography Photosynthesis Plant Leaf Plant Physiology Priority Journal Tropical Rain Forest |
| description |
Understanding how plant carbon metabolism responds to environmental variables such as light is central to understanding ecosystem carbon cycling and the production of food, biofuels, and biomaterials. Here, we couple a portable leaf photosynthesis system to an autosampler for volatile organic compounds (VOCs) to enable field observations of net photosynthesis simultaneously with emissions of VOCs as a function of light. Following sample collection, VOCs are analyzed using automated thermal desorption-gas chromatograph-mass spectrometry (TD-GC–MS). An example is presented from a banana plant in the central Amazon with a focus on the response of photosynthesis and the emissions of eight individual monoterpenes to light intensity. Our observations reveal that banana leaf emissions represent a 1.1 +/- 0.1% loss of photosynthesis by carbon. Monoterpene emissions from banana are dominated by trans-β-ocimene, which accounts for up to 57% of total monoterpene emissions at high light. We conclude that the developed system is ideal for the identification and quantification of VOC emissions from leaves in parallel with CO2 and water fluxes.The system therefore permits the analysis of biological and environmental sensitivities of carbon metabolism in leaves in remote field locations, resulting in the emission of hydrocarbons to the atmosphere. • A field-portable system is developed for the identification and quantification of VOCs from leaves in parallel with leaf physiological measurements including photosynthesis and transpiration. • The system will enable the characterization of carbon and energy allocation to the biosynthesis and emission of VOCs linked with photosynthesis (e.g. isoprene and monoterpenes) and their biological and environmental sensitivities (e.g. light, temperature, CO2). • Allow the development of more accurate mechanistic global VOC emission models linked with photosynthesis, improving our ability to predict how forests will respond to climate change. It is our hope that the presented system will contribute with critical data towards these goals across Earth's diverse tropical forests. © 2020 |
| format |
Artigo |
| author |
Jardine, Kolby J. |
| author2 |
Zorzanelli, Raquel F. Gimenez, Bruno Oliva Robles, Emily Oliveira Piva, Luani Rosa de |
| author2Str |
Zorzanelli, Raquel F. Gimenez, Bruno Oliva Robles, Emily Oliveira Piva, Luani Rosa de |
| title |
Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| title_short |
Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| title_full |
Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| title_fullStr |
Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| title_full_unstemmed |
Development of a portable leaf photosynthesis and volatile organic compounds emission system |
| title_sort |
development of a portable leaf photosynthesis and volatile organic compounds emission system |
| publisher |
MethodsX |
| publishDate |
2020 |
| url |
https://repositorio.inpa.gov.br/handle/1/23315 |
| _version_ |
1787141182526062592 |
| score |
11.755432 |