Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol
Due to search for alternative biomasses, that are low cost and high in cellulose concentration, for the production of 2nd generation ethanol - or lignocellulolytic - this study was carried out with the waste from the green line of slaughterhouse cattle, which is produced on a large scale in Brazil...
| Autor principal: | Siqueira, Lara Neiva de |
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| Grau: | Dissertação |
| Idioma: | pt_BR |
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Universidade Federal do Tocantins
2020
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http://hdl.handle.net/11612/1964 |
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ir-11612-1964 |
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ir-11612-19642020-04-02T06:01:10Z Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol Siqueira, Lara Neiva de Guarda , Emerson Adriano Biomassa; Produção de etanol; Frigorífico; Hidrólise enzimática; Biomass; Ethanol production; Slaughterhouse; Enzymatic hydrolysis CNPQ::CIENCIAS AGRARIAS Due to search for alternative biomasses, that are low cost and high in cellulose concentration, for the production of 2nd generation ethanol - or lignocellulolytic - this study was carried out with the waste from the green line of slaughterhouse cattle, which is produced on a large scale in Brazil and in the State of Tocantins. The biggest challenge faced by companies of cattle slaughter is the large volume of waste that is generated, for instance, in the green line it includes cattle manure, vomiting, stomach contents, intestinal contents, residues of fat purification tank and others and these residues are mostly taken to incinerators or composting. This study, which was conducted at the Universidade Federal do Tocantins, in Palmas city – Tocantins state, aimed to determine the chemical composition of solid biomass from the filtration of all the liquid in the green line and the liquid biomass to evaluate its use in the lignocellulolytic ethanol production. The observed results have shown that the treated solid biomass waste had 57% cellulose, the solid biomass waste without pretreatment and the suspended solids had 30% and 27%, respectively. This demonstrates that the values obtained in laboratory tests were very close to what appears in literature for other residual biomass that were used as a source of cellulose for the production of 2nd generation ethanol. All the biomass were pretreated by autoclaving for 20 minutes at 121 ° C, but only the solid sample underwent a chemical pretreatment before the hydrolysis step. Also, the commercial cellulase enzyme: Cellic CTEC2 was used to carry the enzymatic hydrolysis. The amount of sugars in the sample was determined by HPLC (high-performance liquid chromatography) which has shown a hydrolysis yield of 0,22% for the liquid sample (Li), 2,67% for the solid sample without pretreatment (SI), 69,83% for the solid sample with pretreatment (SII) and 2,4% for the one with suspended solids (Ss). The concentration of ethanol for Li and Ss biomasses was not susceptible to detection by HPLC and the yield of ethanol to SII was 56%, which shows that it has the potential for bioethanol production. Devido à busca por biomassas alternativas, de baixo custo e com elevada concentração de celulose, para produção de etanol de 2ª geração – ou lignocelulolítico – realizou-se este estudo com o resíduo proveniente da linha verde de abatedouros de bovinos, resíduo produzido em larga escala no Brasil e no Estado do Tocantins. O maior desafio enfrentado pelas empresas do ramo do abate de bovinos é o grande volume de resíduos gerados. Os resíduos da linha verde compreendem esterco de currais, vômitos, conteúdo estomacais, conteúdo intestinal, resíduos do tanque de purificação de gorduras e outros. Tais resíduos são expedidos, geralmente, para incineradores ou para compostagem. O estudo foi conduzido na Universidade Federal do Tocantins, Palmas – TO, com objetivo de determinar a composição química da biomassa sólida, proveniente da filtragem de todo líquido da linha verde, bem como da biomassa líquida, para avaliar seu uso na produção de etanol lignocelulolítico. Os resultados observados mostraram que a biomassa residual sólida tratada apresentou 57% de celulose e as biomassas, sólida sem pré-tratamento e sólidos suspensos apresentaram 30% e 27%, respectivamente. Isto demonstra que os valores obtidos nas análises laboratoriais ficaram bem próximos do apresentado na literatura para outras biomassas residuais utilizadas como fonte de celulose na produção do etanol de 2° geração. Todas as biomassas foram pré-tratadas em autoclave por 20 minutos a 121° C, sendo que apenas a amostra sólida passou por um pré-tratamento químico antes do início da etapa de hidrólise. Foi realizada a hidrólise enzimática utilizando-se enzima comercial celulase Cellic CTEC2. A quantidade de açúcares na amostra foi determinada por CLAE obtendo-se rendimentos na hidrólise de 0,22% para a amostra Líquida (Li), 2,67% para a amostra Sólida sem Prétratamento (SI), 69,83% para a amostra Sólida com pré-tratamento (SII) e 2,40% para a amostra sólidos suspensos (Ss). A concentração de etanol para as biomassas Li e Ss não foi passível de detecção por CLAE e o rendimento de etanol para SII foi de 56%, demonstrando que esta biomassa possui potencial para produção de bioetanol. 2020-04-01T19:25:36Z 2020-04-01T19:25:36Z 2016-04-04 Dissertação SIQUEIRA, Lara Neiva de. Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol . 2016.75f. Dissertação (Mestrado em Agroenergia) – Universidade Federal do Tocantins, Programa de Pós-Graduação em Agroenergia, Palmas, 2016. http://hdl.handle.net/11612/1964 pt_BR Open Access application/pdf application/pdf Universidade Federal do Tocantins BR Programa de Pós-Graduação em Agroenergia - PPGA Palmas |
| institution |
Repositório Institucional - Universidade Federal do Tocantins - UFT |
| collection |
RepositorioUFT |
| language |
pt_BR |
| topic |
Biomassa; Produção de etanol; Frigorífico; Hidrólise enzimática; Biomass; Ethanol production; Slaughterhouse; Enzymatic hydrolysis CNPQ::CIENCIAS AGRARIAS |
| spellingShingle |
Biomassa; Produção de etanol; Frigorífico; Hidrólise enzimática; Biomass; Ethanol production; Slaughterhouse; Enzymatic hydrolysis CNPQ::CIENCIAS AGRARIAS Siqueira, Lara Neiva de Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| topic_facet |
Biomassa; Produção de etanol; Frigorífico; Hidrólise enzimática; Biomass; Ethanol production; Slaughterhouse; Enzymatic hydrolysis CNPQ::CIENCIAS AGRARIAS |
| description |
Due to search for alternative biomasses, that are low cost and high in cellulose concentration, for the production of 2nd generation ethanol - or lignocellulolytic - this study was carried out with the waste from the green line of slaughterhouse cattle, which is produced on a large scale in Brazil and in the State of Tocantins. The biggest challenge faced by companies of cattle slaughter is the large volume of waste that is generated, for instance, in the green line it includes cattle manure, vomiting, stomach contents, intestinal contents, residues of fat purification tank and others and these residues are mostly taken to incinerators or composting. This study, which was conducted at the Universidade Federal do Tocantins, in Palmas city – Tocantins state, aimed to determine the chemical composition of solid biomass from the filtration of all the liquid in the green line and the liquid biomass to evaluate its use in the lignocellulolytic ethanol production. The observed results have shown that the treated solid biomass waste had 57% cellulose, the solid biomass waste without pretreatment and the suspended solids had 30% and 27%, respectively. This demonstrates that the values obtained in laboratory tests were very close to what appears in literature for other residual biomass that were used as a source of cellulose for the production of 2nd generation ethanol. All the biomass were pretreated by autoclaving for 20 minutes at 121 ° C, but only the solid sample underwent a chemical pretreatment before the hydrolysis step. Also, the commercial cellulase enzyme: Cellic CTEC2 was used to carry the enzymatic hydrolysis. The amount of sugars in the sample was determined by HPLC (high-performance liquid chromatography) which has shown a hydrolysis yield of 0,22% for the liquid sample (Li), 2,67% for the solid sample without pretreatment (SI), 69,83% for the solid sample with pretreatment (SII) and 2,4% for the one with suspended solids (Ss). The concentration of ethanol for Li and Ss biomasses was not susceptible to detection by HPLC and the yield of ethanol to SII was 56%, which shows that it has the potential for bioethanol production. |
| author_additional |
Guarda , Emerson Adriano |
| author_additionalStr |
Guarda , Emerson Adriano |
| format |
Dissertação |
| author |
Siqueira, Lara Neiva de |
| title |
Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| title_short |
Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| title_full |
Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| title_fullStr |
Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| title_full_unstemmed |
Aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| title_sort |
aproveitamento de biomassa residual lignocelulolítica da indústria de abate de bovinos para obtenção de bioetanol |
| publisher |
Universidade Federal do Tocantins |
| publishDate |
2020 |
| url |
http://hdl.handle.net/11612/1964 |
| _version_ |
1787134531567878144 |
| score |
11.755432 |