Dissertação

Eficiência de Bacillus subtilis no biocontrole de fitopatógenos e promotor de crescimento vegetal

The rhizobacteria promote plant growth (RBCP) colonize roots of plants and induce an increase in plant crecimento by different mechanisms. The rizobacteria Bacillus subtilis is able to act as a control agent of several cultivated plant diseases, as well as plant growth promoter. Thus, this study aim...

ver descrição completa

Autor principal: Braga Júnior, Gaspar Moreira
Grau: Dissertação
Idioma: pt_BR
Publicado em: Universidade Federal do Tocantins 2017
Assuntos:
Acesso em linha: http://hdl.handle.net/11612/386
Resumo:
The rhizobacteria promote plant growth (RBCP) colonize roots of plants and induce an increase in plant crecimento by different mechanisms. The rizobacteria Bacillus subtilis is able to act as a control agent of several cultivated plant diseases, as well as plant growth promoter. Thus, this study aimed to the four chapters presented, to evaluate the mycelial growth of pathogens such as the ability of isolates of B. subtilis to inhibit these pathogens; evaluate the effect of isolates of B. subtilis inoculated in soil fertilized with rock phosphate and without fertilization, as its ability to provide P, the growth of soybean and cowpea at home conditions of vegetation; evaluate the efficiency of B. subtilis inoculation as plant growth promoter in corn in a greenhouse; and evaluate the efficiency of inoculation of B. subtilis the agronomic performance of soybeans under field conditions in two locations. They tested seven isolates of B. subtilis pathogens Fusarium subglutinans, Curvularia lunata and Bipolaris spp., assessing the biological control capacity in vitro. In the evaluation of growth inhibition ability of the pathogens using four methods isolates of B. subilis UFTBs 03, UFTBs 05, UFTBs 06, UFTBs 07 were effective. Isolated UFTBs 01, UFTBs 03, UFTBs 04, UFTBs 05, UFTBs 06 and 07 UFTBs were able to inhibit the mycelial growth of the pathogens tested by thermostable metabolites, antibiose being its main mechanism of action. As the efficiency of inoculation of B. subtilis in soybean and cowpea with natural phosphate fertilizer and no fertilization in a greenhouse, using seven isolates and MIX, it was observed that most of the treatments which received inoculation of B. subtilis provided the growth of soybean and cowpea, also most of the isolates tested provided a higher P content available in soil and shoots of plants, both in soil supplemented with rock phosphate as well as in soil fertilization in both cultures. In greenhouse testing inoculant B. subitilis, composed of three strains (UFTBs 01, UFTBs 02, UFTBs 03) in corn, showed that maize seed inoculated with B. subtilis resulted in plants with higher biomass accumulation in stadium initial growth. In the field trial, where he was tested inoculant B. subtilis, composed of three strains (UFTBs 01, UFTBs 02, UFTBs 03) in soybean at two sites in the harvest 2013/1014, inoculation with B. subtilis gave rise biomass and soybean yield in the two regions where they were evaluated. The results of this study demonstrated the efficacy of B. subtilis native isolated from the Tocantins soil, the biological control of plant pathogens, as well as the capacity and bean growth promoters cowpea, corn and soybeans, and increased the soybean yield.