The aim of these studies was to evaluate the seminal parameters and the electrophoretic
profile of the seminal plasma of males tambaqui, induced with pituitary carp extract (0.5 mg /
kg) and correlate with possible seminal quality markers. The improvement of already existing
biotechniques of reproduction for tambaqui, as well as the development of methodologies
with these studies, will be able to improve the programs of genetic improvement and
reproductive efficiency of the commercial plants. In addition, they will serve to assist
programs of conservation and recovery of endangered species. Seminal collections took place
at Fazenda São Paulo, aquaculture located in the county of Brejinho de Nazaré, Tocantins.
This study was approved by the animal use ethics committee of the Federal University of
Tocantins (protocol number 23101.001692 / 2015-65). Among the evaluated parameters, the
mean seminal volume was 3.31 mL / 4.18 ± 0.46 kg of body weight and prevalence of milky
appearance (62.5%). The mean motility was 78.75% and the mean vigor on the 3.5 scale. The
mean spermatic concentration was 12.68 x 109
spermatozoa / mL, with minor defects were on
average 5.50% and major defects in 2.56% of spermatozoa, with an average of 84.50%
spermatozoa being intact through the eosin-nigrosine test. The sperm viability test was carried
out by the application of a new methodology, without the need to heat the dye solution and
the semen at 37 ° C and without the preheating of the microscopic slides. With the
electrophoretic profile of the seminal plasma, a total of 26 protein bands with molecular
weights ranging from 9.87 to 221.93 kDa were detected, with a predominance of proteins with
low molecular weights. The presence of low molecular weight proteins is an indication of
seminal quality and fertility, and serves as a seminal quality parameter for use in
cryopreservation in germplasm banks of this species.
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